Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3

Didriksen, Allan; Burild, Anders; Jakobsen, Jette; Fuskevåg, Ole-Martin; Jorde, Rolf

doi:10.1530/eje-14-0870

Cited by 71 publications

(56 citation statements)

References 20 publications

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“…Extraction efficiencies of the [6, 19, 19‐d 3 ]‐vitamin D3 and [6, 19, 19‐d 3 ]‐vitamin D2 were similar and averaged 58 ± 5%. These estimates for recovery and accuracy were comparable to other published results …”

Section: Methodssupporting

confidence: 88%

“…Prior studies on vitamin D storage in adipose tissue in non‐obese and obese subjects have also focused primarily on subcutaneous fat . Heaney and colleagues investigated the concentration of vitamin D2 and D3 in the subcutaneous tissue of non‐obese but overweight individuals and also found that subjects had greater levels of D3 compared with D2 in their adipose tissue .…”

Section: Discussionsupporting

confidence: 77%

“…We found a significant direct correlation between serum 25OHD and total vitamin D in both subcutaneous and omental fat and found a similar relationship in both the obese and non‐obese subjects. These data amplify similar findings from most but not all prior studies, which assessed primarily subcutaneous adipose tissue and reported associations with both 25OHD and serum vitamin D3 . Similarly, in a recent animal study, serum and adipose vitamin D were closely related …”

Section: Discussionmentioning

confidence: 99%

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Vitamin D Storage in Adipose Tissue of Obese and Normal Weight Women

Carrelli

Bucovsky

Horst³

et al. 2016

Journal of Bone and Mineral Research

156

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Although vitamin D deficiency is prevalent among obese individuals, its cause is poorly understood. Few studies have measured vitamin D concentrations in adipose of obese (OB) subjects, and none have included normal weight controls (C). The goal of this study was to investigate whether the relationship between body composition, serum 25-hydroxyvitamin D (25OHD), vitamin D in subcutaneous (SQ) and omental (OM) adipose, and total adipose stores of vitamin D differ among OB and C. Obese women undergoing bariatric surgery and normal-weight women undergoing abdominal surgery for benign gynecologic conditions were enrolled. Subjects had measurements of serum 25OHD by high-performance liquid chromatography (HPLC) and body composition by dual-energy X-ray absorptiometry (DXA). Vitamin D concentrations in SQ and OM adipose were measured by mass spectroscopy. Thirty-six women were enrolled. Serum 25OHD was similar between groups (OB 27 ± 2 versus C 26 ± 2 ng/mL; p = 0.71). Adipose vitamin D concentrations were not significantly different in either SQ (OB 34 ± 9 versus C 26 ± 12 ng/g; p = 0.63) or OM compartments (OB 51 ± 13 versus C 30 ± 18 ng/g; p = 0.37). The distribution of vitamin D between SQ and OM compartments was similar between groups. Serum 25OHD was directly related to adipose vitamin D in both groups. Total body vitamin D stores were significantly greater in OB than in C (2.3 ± 0.6 versus 0.4 ± 0.8 mg, respectively; p < 0.01).In summary, although OB had significantly greater total vitamin D stores than C, the relationship between serum 25OHD and fat vitamin D and the overall pattern of distribution of vitamin D between the OM and SQ fat compartments was similar. Our data demonstrate that obese subjects have greater adipose stores of vitamin D. They support the hypotheses that the enlarged adipose

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Section: Methodssupporting

confidence: 88%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin D Storage in Adipose Tissue of Obese and Normal Weight Women

Carrelli

Bucovsky

Horst³

et al. 2016

Journal of Bone and Mineral Research

156

View full text Add to dashboard Cite

show abstract

“…Although adipose tissue contains substantial amounts of vitamin D [34], very little is known about the storage capacity and how the storage and mobilization processes are regulated. Based on the analysis of vitamin D in fat biopsies from subjects living in northern Norway (latitude 70°) who had participated in an RCT for 3-5 years, Didriksen et al [35] recently estimated that the stores of vitamin D in subcutaneous fat tissue were equivalent to 90 daily doses of 10 lg in the placebo group and 328 daily doses of 20 lg in the group taking vitamin D (oral dose of 500 lg per week). The observations of a clear positive association between vitamin D concentration in fat and serum and a linear increase in circulating 25OHD levels as a function of adiposity volume loss indicate bioavailability of cholecalciferol in fat stores during periods of insufficiency [32,[35][36][37][38].…”

mentioning

confidence: 99%

The seasonal importance of serum 25‐hydroxyvitamin D for bone mineral density in older women

et al. 2016

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“…The hormone 1,25(OH) 2 D 3 and its metabolites are lipophilic and are stored in adipose tissue, which might explain the low serum levels of this hormone in obese individuals [10,11]. Didriksen et al [12] demonstrated that after treatment with 1,25(OH) 2 D 3 at 20,000 IU/week for 3-5 years, adipose tissue from obese individuals with impaired glucose tolerance and/ or impaired fasting glucose contains six-fold higher 1,25(OH) 2 D 3 levels than adipose tissue from a placebo group.…”

Section: Introductionmentioning

confidence: 99%

Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation

Felicidade¹,

Sartori

Coort

et al. 2018

Cell Physiol Biochem

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Background/Aims: Compared with non-obese individuals, obese individuals commonly store more vitamin D in adipose tissue. VDR expression in adipose tissue can influence adipogenesis and is therefore a target pathway deserving further study. This study aims to assess the role of 1,25(OH)₂D₃ in human preadipocyte proliferation and differentiation. Methods: RTCA, MTT, and trypan blue assays were used to assess the effects of 1,25(OH)₂D₃ on the viability, proliferation, and adipogenic differentiation of SGBS cells. Cell cycle and apoptosis analyses were performed with flow cytometry, triglycerides were quantified, and RT-qPCR was used to assess gene expression. Results: We confirmed that the SGBS cell model is suitable for studying adipogenesis and demonstrated that the differentiation protocol induces cell maturation, thereby increasing the lipid content of cells independently of treatment. 1,25(OH)₂D₃ treatment had different effects according to the cell stage, indicating different modes of action driving proliferation and differentiation. In preadipocytes, 1,25(OH)₂D₃ induced G1 growth arrest at both tested concentrations without altering CDKN1A gene expression. Treatment with 100 nM 1,25(OH)₂D₃ also decreased MTT absorbance and the lipid concentration. Moreover, increased normalized cell index values and decreased metabolic activity were not induced by proliferation or apoptosis. Exposure to 100 nM 1,25(OH)₂D₃ induced VDR, CEBPA, and CEBPB expression, even in the preadipocyte stage. During adipogenesis, 1,25(OH)₂D₃ had limited effects on processes such as VDR and PPARG gene expression, but it upregulated CEBPA expression. Conclusions: We demonstrated for the first time that 1,25(OH)₂D₃ induces changes in preadipocytes, including VDR expression and growth arrest, and increases the lipid content in adipocytes treated for 16 days. Preadipocytes are important cells in adipose tissue homeostasis, and understanding the role of 1,25(OH)₂D₃ in adipogenesis is a crucial step in ensuring adequate vitamin D supplementation, especially for obese individuals.

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Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3

Cited by 71 publications

References 20 publications

Vitamin D Storage in Adipose Tissue of Obese and Normal Weight Women

Vitamin D Storage in Adipose Tissue of Obese and Normal Weight Women

The seasonal importance of serum 25‐hydroxyvitamin D for bone mineral density in older women

Role of 1α,25-Dihydroxyvitamin D3 in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation

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