Vitamin D3- Induced Histological Changes in the Corpuscles of Stannius of a Freshwater Catfish, Heteropneustes Fossilis Kept Either in Artificial Freshwater, Calcium-rich Freshwater or Calciumdeficient Freshwater

Abstract

Summary: Vitamin D3 (50I.U./100 g body wt) was injected day intraperitoneally to the fish H. fossilis maintained in artificial freshwater, calcium-rich freshwater and calcium deficient freshwater. The animals were killed on day 1, 3, 5, and 10. The serum calcium levels were estimated and CS were fixed for histological studies.Administration of vitamin D3 induced hypercalcemia in the fish kept in all the three different media.The AF-positive cells of CS of vitamin D3 treated specimens kept in artificial freshwater, calcium-rich freshwater and calcium-deficient freshwater depict hyperactivity which is expressed by their degranulation and increased nuclear volume.The AF-negative cells of CS of vitamin D3-treated fish kept in artificial freshwater have not shown any change, however, the AF-negative cells of the fish treated with vitamin D3 and maintained in calcium-rich freshwater and calciumdeficient freshwater exhibit a decrease in their nuclear volume.Corpuscles of Stannius (CS) are endocrine tissue which exist only in the kidneys of some holostei and teleostei. Functional aspects of this gland is not yet well understood but the studies of Wendelaar Bonga and

Alterations in the Corpuscles of Stannius of Euphorbia royleana Treated Catfish, Heteropneustes fossilis

“…Accumulation of AF-positive granules in CS derives support from the earlier investigators in which similar observation is noticed in response to experimentally induced hypocalcemia in fishes kept in ambient acalcic freshwater [33,35]. In mammals, accumulation of secretory granules in the calcitonin cells (responsible for the secretion of a hypocalcemic factor-CT) has also been noticed in response to hypocalcemia [36][37][38][39].…”

Three different levels of dietary vitamin D3fed to first-feeding fry of Atlantic salmon (Salmo salarL.): effect on growth, mortality, calcium content and bone formation

“…However, in most of these studies, normalization of the calcium levels occurred after 5–10 days. This stabilization was accompanied with release of the hypocalcaemic factor, stanniocalcin, from the corpuscles of Stannius in the kidney (Singh & Srivastav 1996; Srivastav et al. 1998).…”

Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D₃

“…Until recently, stanniocalcin was considered to be a fish hormone involved in the maintenance of Ca 2+ homeostasis through prevention of hypercalcemia by inhibiting Ca 2+ uptake from the gill and intestine and by stimulating phosphate reabsorption in the kidney. Fish stanniocalcin has been extensively characterized in terms of its structure, regulation of expression and secretion, and physiological actions [3, 4, 5, 9, 10, 11, 12, 13, 14, 15]. …”