Vitamin E Depletion Enhances Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress

Ohta, Yoshiji; Yashiro, Koji; Ohashi, Koji; Imai, Yoichiro; Kusumoto, Chiaki; Matsura, Tatsuya; Hidaka, Mayumi; Fukuzawa, Kenji

doi:10.3177/jnsv.59.79

Cited by 4 publications

(13 citation statements)

References 32 publications

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“…Exposure to 6 h of WIRS caused a significant increase in serum ascorbic acid concentration in ND-fed rats, as reported previously (9,17). A significant increase in serum ascorbic acid concentration was also found in VESD-fed rats with 6 h of WIRS.…”

Section: Discussionsupporting

confidence: 72%

“…In addition, VESDfed rats showed a more marked change in a-tocopherol concentration in the liver tissue than in the serum. Exposure to 6 h of WIRS did not affect serum a-tocopherol concentration either in ND-fed rats, as reported previously (10,17), or in VESD-fed rats. In contrast, exposure to 6 h of WIRS did not cause any change in serum a-tocopherol concentration in ND-fed rats, as reported previously (10,17), but caused further increase in liver a-tocopherol concentration in VESDfed rats.…”

Section: Discussionsupporting

confidence: 62%

“…In the present study, ND-fed rats with 6 h of WIRS showed increases in the serum levels of stress makers such as ACTH, corticosterone, and glucose, as shown in our previous reports (9,10,17,21). In rats fed the VESD for 4 wk, exposure to 6 h of WIRS also increased the serum levels of ACTH, corticosterone, and glucose.…”

Section: Discussionsupporting

confidence: 57%

“…Recently, we have reported that exposure of rats fed a normal diet and vitamin E-depleted diet for 4 wk to 6 h of WIRS reduces CoQ9red and CoQ10red levels in the liver tissue and that this vitamin E depletion enhances liver oxidative damage in rats with 6 h of WIRS through disruption of the liver non-enzymatic antioxidant defense system, although the vitamin E depletion causes increases in liver ascorbic acid and GSH levels in unstressed rats (17). However, there is no information on whether dietary vitamin E supplementation protects against WIRS-induced oxidative damage in the liver of rats.…”

Section: Effect Of Dietary Vitamin E Supplementation On Liver Oxidatimentioning

confidence: 99%

“…It has been reported that oral supplementation of vitamin E for 28 d protects against gastric mucosal damage by reducing stress response, judging from the plasma levels of stress markers such as adrenocorticotrophic hormone (ACTH), corticosterone, adrenaline, and noradrenaline in rats with 3.5 h of WIRS (18,19). It is known that exposure of rats to 6 h of WIRS increases not only the serum or plasma levels of stress markers such as ACTH, corticosterone, adrenaline, noradrenaline, and glucose levels but also serum ascorbic acid and LPO levels (9,10,17,20). It is also known that a single oral administration of vitamin E to rats with 6 h of WIRS has no effect on the serum levels of stress markers such as ACTH, corticosterone, and glucose but attenuates increased serum ascorbic acid and LPO levels (10,21).…”

Section: Effect Of Dietary Vitamin E Supplementation On Liver Oxidatimentioning

confidence: 99%

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Effect of Dietary Vitamin E Supplementation on Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress

Ohta

Yashiro

Ohashi

et al. 2015

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Water-immersion restraint stress (WIRS), which is characterized with both psychological stress and physical stress, is widely used to induce reproducible stress ulcers in experimental animals (1). Reactive oxygen species (ROS) and nitric oxide ( • NO) generation, lipid peroxidation, and neutrophil infiltration in the gastric mucosal tissue play a critical role in the pathogenesis of WIRS-induced gastric mucosal lesions (2-5). Iwai et al. (6) have reported that, in rats with 6 h of WIRS, the level of liver lipid peroxide (LPO), which is generated via ROS, increases with concomitant increases in the levels of serum LPO and liver cell damage markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities and that pre-administration of gamazumi fruit juice or (2)-epigallocatechin gallate, each of which possesses antioxidant activity, for 2 wk attenuates all these stress-induced changes. Furthermore, the same authors have reported that not only an increase in LPO level but also decreases in superoxide dismutase (SOD) activity and reduced glutathione (GSH) level occur without changes in catalase and glutathione peroxidase activities in the liver of rats with 6 h of WIRS and that preadministration of gamazumi crude extract possessing antioxidant activity to rats with 6 h of WIRS for 2 wk attenuates the increased LPO level and the decreased SOD activity and GSH level in the liver (7). We have reported that, in the liver of rats exposed to WIRS over a 6-h period, cell damage occurs before the appearance of oxidative stress with disruption of antioxidant defense systems associated with decreased ascorbic acid (vitamin C) and GSH levels and SOD activity and enhanced lipid peroxidation, enhanced • NO generation, and neutrophil infiltration in the tissue, although the vitamin E level does not change in the liver tissue (8). We have also shown that a single pre-administration of l-ascorbic acid protects against oxidative damage in the liver of rats with 6 h of WIRS by attenuating disrupted antioxidant defense systems and enhanced lipid peroxidation, enhanced• NO generation, and neutrophil infiltration in Asaminami-ku, Hiroshima 731-0153, Japan (Received October 20, 2014) Summary We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU a-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver a-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q9 (CoQ9), reduced CoQ9, reduced CoQ10, and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities incr...

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Section: Discussionsupporting

confidence: 72%

Section: Discussionsupporting

confidence: 62%

Section: Discussionsupporting

confidence: 57%

Section: Effect Of Dietary Vitamin E Supplementation On Liver Oxidatimentioning

confidence: 99%

Section: Effect Of Dietary Vitamin E Supplementation On Liver Oxidatimentioning

confidence: 99%

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Effect of Dietary Vitamin E Supplementation on Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress

Ohta

Yashiro

Ohashi

et al. 2015

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Assessment of genotoxicity associated with Behcet’s disease using sister-chromatid exchange assay: vitamin E versus mitomycin C

2014

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Behcet's disease (BD) is a multisystemic chronic inflammatory disorder that presents throughout the world with high frequency in Turkey and Middle East. BD has been shown to be associated with genotoxicity as patients with the disease have demonstrated high rates of sister chromatid exchange (SCE) and oxidative DNA damage. In this study, we examined the effect of vitamin E, which is known for its strong antioxidant activity, on the rate of SCE in cultured lymphocytes obtained from BD patients. In addition, the susceptibility of patient lymphocytes to the mutagenic agent mitomycin C (MMC) was also investigated. The results showed significant elevation in the rate of SCE in lymphocytes obtained from patients compared to those from healthy subjects (P < 0.01). Treatment with vitamin E normalized the elevated rate of SCE to a comparable level observed in the control group (P < 0.01). Finally, treatment of cultures with MMC significantly increased the rate of SCE in the lymphocytes of both patients and controls (P < 0.001). The magnitude of change in the rate of SCE induced by MMC was equivalent in both groups. This result suggests similar sensitivity of BD lymphocytes and control ones to MMC. In conclusion, genotoxicity associated with BD can be overcome by treatment with vitamin E. Lymphocytes of BD have normal sensitivity to the mutagenic agent MMC.

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Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats

Hou

Liang

et al. 2015

Int J Immunopathol Pharmacol

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Chronic stress can suppress natural killer (NK) cell activity; this may also be related to the effect of stress on the neuroendocrine-immune network. Sea buckthorn (SBT) (Hippophae rhamnoides L.) is a thorny nitrogen fixing deciduous shrub, native to both Europe and Asia. It has been used as a medicinal plant in Tibetan and Mongolian traditional medicines. SBT has multifarious medical properties, including anti-fatigue as well as immunoregulatory effects. This study reports the effects of SBT oil with regard to the cytotoxicity and quantity of NK cells in the blood of a chronic-stress rat model, in addition to its mechanisms on the neuroendocrine-immune network. These results show that SBT oil, given by gavage to rats with chronic stress, could increase the following: body weight, NK cell quantities, and cytotoxicity, as well as the expression of perforin and granzyme B. The results also show that SBT oil in rats with chronic stress could suppress cortisol, ACTH, IL-1β and TNF-α levels, in addition to increasing 5-HT and IFN-γ serum levels. This leads to suggest that SBT oil, in rats with chronic stress, can increase NK cell cytotoxicity by upregulating the expression of perforin and granzyme B, thus causing associated effects of SBT oil on the neuroendocrine-immune network.

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Vitamin E Depletion Enhances Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress

Cited by 4 publications

References 32 publications

Effect of Dietary Vitamin E Supplementation on Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress

Effect of Dietary Vitamin E Supplementation on Liver Oxidative Damage in Rats with Water-Immersion Restraint Stress

Assessment of genotoxicity associated with Behcet’s disease using sister-chromatid exchange assay: vitamin E versus mitomycin C

Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats

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