Vitamin E improves antioxidant status but not lipid metabolism in laying hens fed a aged corn-containing diet

Abstract

Objective: The objective of this study was to determine whether a dietary vitamin E (VE) supplement could alleviate any detrimental effects of aged corn on lipid metabolism and antioxidant status in laying hens.Methods: The experiment consisted of a 2×3 factorial design with two corn types (normal corn and aged corn (stored for 4 yr) and three concentrations of VE (0, 20, and 100 IU/kg). A total of 216 Lohmann laying hens (50 wk of age) were randomly allocated into six treatment diets for 12 wk. Each treatment had 6 replicates of 6 hens per replicate.Results: The results show that aged corn significantly decreased the content of low-density lipoprotein cholesterol (p<0.05), and reduced chemokine-like receptor 1 (CMKLR1) mRNA expression (p<0.05) in the liver compared to controls. Diet with VE did not alter the content of crude fat and cholesterol (p>0.05), or acetyl-CoA carboxylase, lipoprotein lipase, fatty acid synthase or CMKLR1 mRNA expression (p>0.05) in the liver among treatment groups. Aged corn significantly increased the content of malondialdehyde (MDA) (p<0.05) and decreased superoxide dismutase (SOD) activity (p<0.05) in the liver. The VE increased the content of MDA (p<0.05) but decreased glutathione peroxidase (GSH-Px) activity in serum (p<0.01) and in the ovaries (p<0.05). Adding VE at 20 and 100 IU/kg significantly increased GSH-Px activity (p<0.05) in liver and in serum (p<0.01), 100 IU/kg VE significantly increased SOD activity (p<0.05) in serum. Aged corn had no significant effects on <i>GSH-Px</i> mRNA or <i>SOD</i> mRNA expression (p<0.01) in the liver and ovaries. Addition of 100 IU/kg VE could significantly increase <i>SOD</i> mRNA expression (p<0.01) in the liver and ovary.Conclusion: Aged corn affected lipid metabolism and decreased the antioxidant function of laying hens. Dietary VE supplementation was unable to counteract the negative effects of aged corn on lipid metabolism. However, addition of 100 IU/kg VE prevented aged corninduced lipid peroxidation in the organs of laying hens.

Effects of stocking density and dietary vitamin C on performance, meat quality, intestinal permeability, and stress indicators in broiler chickens

“…Protein concentrations were also analyzed using a commercial kit (Thermo Fisher Scientific) [ 24 ]. The relative concentrations of MDA and TAC to protein concentrations were calculated and expressed as µmol/mg and U/mg protein, respectively [ 25 ].…”

The Role of Chemerin in Metabolic and Cardiovascular Disease: A Literature Review of Its Physiology and Pathology from a Nutritional Perspective

“…Vitamin C did not affect chemerin in adipocytes [ 65 ], and vitamin K absence in hepatocellular carcinoma patients did not alter chemerin [ 66 ]. Additionally, vitamin B3 increased chemerin mRNA levels in differentiated bovine preadipocytes [ 67 ], while vitamin E supplementation upregulated hepatic CMKLR1 mRNA expression [ 68 ].…”

Effects of quercetin and daidzein on egg quality, lipid metabolism, and cecal short-chain fatty acids in layers

“…Lipids are not synthesized in the ovaries of laying hens. VLDL is the main carrier of cholesterol in the serum, takes charge of transferring cholesterol from the serum to the ovary, and then is absorbed by the oocyte to form egg yolk, thus synthesizing 95% of the cholesterol in egg yolk (46,47). Therefore, the contents of TG, TC, HDL, LDL, and VLDL in the serum were used to estimate whether lipid metabolism is normal in laying hens.…”