Vitamin E prevents steroid-induced osteonecrosis in rabbits

Abstract

Background and purposePrevention of osteonecrosis after corticosteroid administration would be important. We examined the potential of vitamin E (α-tocopherol) to reduce the incidence of corticosteroid-induced osteonecrosis in an animal model.MethodsJapanese white rabbits were divided into 2 groups; the control group was fed a normal diet and the experimental group was fed α-tocopherol-supplemented diet in which α-tocopherol (600 mg/kg diet) was added to the normal diet. To induce osteonecrosis, high-dose methylprednisolone acetate (MPSL) (20 mg/kg body weight) was injected once into the right gluteus medius muscle of all rabbits. 4 weeks after the injection of MPSL, the presence or absence of osteonecrosis of bilateral femurs was examined histopathologically. The tocopherol/cholesterol ratios were calculated. The plasma levels of thiobarbituric acid-reactive substances (TBARS) were measured.ResultsAlpha-tocopherol-supplemented diet reduced the incidence of osteonecrosis, which developed in 14 of 20 rabbits in the control group and 5 of 21 rabbits in the experimental group (p = 0.004). The tocopherol/cholesterol ratio was higher in the experimental group than in the control group after the α-tocopherol administration. The plasma TBARS level was lower in the experimental group than in the control group at 4 weeks after the MPSL administration.InterpretationOur findings may offer a new approach for the prevention of corticosteroid-induced osteonecrosis.

Biomarkers and pharmaceutical strategies in steroid-induced osteonecrosis of the femoral head: A literature review

“…These theories include vascular occlusion or ischaemia, altered fat metabolism, fat emboli and intravascular coagulation. 1,[3][4][5][6][7][8][9] Biomarkers Lipoprotein. Patients with ONFH were found to have lower serum apolipoprotein A-IV concentrations compared with healthy volunteers, and these concentrations increased with stage of disease.…”

“…[33][34][35] Vitamin E significantly inhibited steroid-induced oxidative stress and the incidence of osteonecrosis in animal models of ONFH. 9,36 Vitamin E may suppress vascular damage by reducing oxidative stress in the blood and blood vessels, thereby maintaining antiplatelet and anticoagulant effects.…”

“…1,[3][4][5][6][7][8][9] There are several known biomarkers for ONFH [10][11][12][13][14][15][16][17][18] Multiple biomarkers are required for disease development, 10 but there is little information available regarding protein-protein interaction in this context.…”

Pengaruh Hiperglikemia dan Vitamin E pada Kadar Malonaldehida dan Enzim Antioksidan Intrasel Jaringan Pankreas Tikus

“…8 Hasil penelitian Kuribayashi dkk. 18 menyatakan bahwa pemberian vitamin E dapat mengurangi pembentukan MDA dan kerusakan pembuluh darah pada kelinci akibat stres oksidatif yang diinduksi dengan kortikosteroid.…”

Osteonecrosis of the femoral head: An update in year 2012

“…Cryogenic and thermal insults have been used to induce osteonecrosis in quadrupeds such as canines [60] and bipeds such as emus [61] . Intramuscular injection of methylprednisolone has been used to develop steroid-induced osteonecrosis in mouse [62] , rat [63] , rabbit [64,65] , pig [66] , and chicken models [18] , where the percent incidence of induced osteonecrosis is dependent on the amount of methylprednisolone injected. These animal models have been used to study the molecular mechanisms of osteonecrosis and assess the usefulness of several therapies over the last few decades.…”