Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw–induced DNA damage

Kalthur, Guruprasad; Raj, Sujith; Thiyagarajan, A.; Kumar, Sampath; Kumar, Pratap; Adiga, Satish Kumar

doi:10.1016/j.fertnstert.2010.10.005

Cited by 93 publications

(63 citation statements)

References 17 publications

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“…Decrease in motility and poor survival of frozen-thawed spermatozoa has been consistently observed in earlier studies [11,12]. Improving the motility in frozen-thawed spermatozoa and maintaining their survival till fertilization is a real challenge in assisted reproduction.…”

Section: Discussionmentioning

confidence: 64%

Supplementation of biotin to sperm preparation medium increases the motility and longevity in cryopreserved human spermatozoa

Kalthur¹,

Salian²,

Keyvanifard³

et al. 2012

J Assist Reprod Genet

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Purpose To study the effect of supplementing biotin to sperm preparation medium on the motility of frozen-thawed spermatozoa. Methods Semen samples of men attending the University infertility clinic (n0105) were cryopreserved using glycerolegg yolk-citrate buffered cryoprotective medium in liquid nitrogen. After a period of two weeks, the semen samples were thawed and the motile spermatozoa were extracted by swim-up technique using Earle's balanced salt solution (EBSS) medium supplemented with either biotin (10 nM) or pentoxifylline (1 mM). The post-wash motility was observed up to 4 h after incubation. Results Both biotin and pentoxifylline supplementation resulted in significant increase in total motility (p<0.05), progressive motility (p<0.001) and rapid progressive motility (p<0.05 v/s biotin and p<0.01 v/s pentoxifylline) compared to the control at 1 h post-incubation period. Significantly higher percentage of total (p<0.01, p<0.05 in biotin and pentoxifylline respectively), progressive (p<0.001) and rapid progressive motilities (p<0.01) were observed in these two groups even at 2 h compared to the control. In the control group at 4 h after incubation,~11% decline in total motility and 8% decline in progressive motility was observed. However, in both biotin and pentoxifylline group the motility was significantly higher than control (p<0.001). No significant difference in the motility was observed between biotin and pentoxifylline groups at any of the time intervals studied. Conclusions Biotin can enhance the sperm motility and prolong the survival of frozen-thawed semen samples which may have potential benefit in assisted reproductive technology field.

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Section: Discussionmentioning

confidence: 64%

Supplementation of biotin to sperm preparation medium increases the motility and longevity in cryopreserved human spermatozoa

Kalthur¹,

Salian²,

Keyvanifard³

et al. 2012

J Assist Reprod Genet

Self Cite

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“…Regardless of how spermatozoa deteriorate, sperm handling causes prolonged exposure of the cells to light (singlet oxygen formation) and oxygen that can create an oxidative environment and lead to detrimental peroxidative processes ( Kalthur et al 2011). Such optimisation of processing techniques has resulted in increased fertility rates in numerous species, particularly with regard to liquid stored semen.…”

Section: Stabilisation Of the Sperm Surface With Cholesterol And Othementioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

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Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

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“…Therefore, protecting the spermatozoa from cryopreservation-induced DNA damage and loss of sperm functional competence is clinically relevant in assisted reproductive technology (ART). Taking this into consideration, the most common approach followed hitherto, was to protect the sperm DNA during freeze-thaw process by using free radical scavengers along with the cryopreservation medium [12,13].…”

Section: Introductionmentioning

confidence: 99%

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Kotdawala

Kumar

Salian

et al. 2012

J Assist Reprod Genet

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Purpose To study the effect of addition of zinc to human semen sample prior to cryopreservation on post-thaw sperm quality and function. Methods Semen samples were collected from men attending university infertility clinic for semen analysis (n0109). Liquefied semen samples were cryopreserved in glycerol-egg yolkcitrate medium with or without the prior addition of zinc (100 μM) and stored in liquid nitrogen. After 10 days, the semen samples were thawed to assess the outcome. Sperm motility, DNA integrity, mitochondrial potential and the ability of spermatozoa to undergo capacitation and acrosome reaction was assessed in post-thaw samples. Results Semen samples cryopreserved after addition of zinc had a significantly higher percentage of sperm with intact DNA (p<0.001), mitochondrial function (p<0.001) and progressive motility (p<0.01) compared to the semen samples cryopreserved without zinc supplementation. Apart from this, ability to undergo capacitation and acrosome reaction in vitro was significantly higher in semen samples cryopreserved with zinc (p<0.0001 and p<0.001 respectively).Conclusions Addition of zinc to semen samples prior to cryopreservation helps in preventing the freeze-thaw-induced sperm DNA damage and loss of sperm function.

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Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw–induced DNA damage

Cited by 93 publications

References 17 publications

Supplementation of biotin to sperm preparation medium increases the motility and longevity in cryopreserved human spermatozoa

Supplementation of biotin to sperm preparation medium increases the motility and longevity in cryopreserved human spermatozoa

Sperm surface changes and physiological consequences induced by sperm handling and storage

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

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