Vitamin Value Assay, Comparison of Vitamin B₆ Values of Selected Food Samples by Bioassay and Microbiological Assay

Vitamin B6 components in some meats, fish, dairy products, and commercial infant formulas

“…The total of the three components gave a true B6 value as compared to the value obtained without chromatographic separation. A previous report (Toepfer et al, 1963) showed agreement between values obtained by chromatography and by rat bioassay.…”

“…Methods have been described for using Dowex 50 ion exchange columns to separate the vitamin B6 components of hydrolyzed food extracts into individual fractions and assaying the separated components with 5. car/sbergensis (MacArthur and Lehmann, 1959;Polansky et al, 1964; Toepfer and Lehmann, 1961). Data obtained by these procedures compare well with those obtained by rat bioassay for total vitamin B6 in a few selected food samples (Toepfer et al, 1963).…”

Vitamin B6 content of feeds

“…It was of interest to examine the pyridoxamine, pyridoxal, and pyridoxine contents of the few feedstuffs for which data are available to see whether this might be related to the type of extraction required for maximum liberation of vitamin Be. A number of studies (Polansky et al, 1964;Ra'oinowitz and Snell, 1948;Toepfer et al, 1960Toepfer et al, , 1963 have revealed wide variations in the relative amounts of the three forms of vitamin B6 from one material to another. There is some indication that 0.0557V acid extraction is more effective where pyridoxamine is predominant (Brewers' dried yeast and fish meal) or where pyridoxal is the major component (meat and bone meal).…”

Development of a LC-MS/MS method using stable isotope dilution for the quantification of individual B6 vitamers in fruits, vegetables, and cereals

“…This is in accordance with reported data, where PNG (0.014-0.149 mg/100 g) accounts for the major part of B 6 in various flour samples, e.g., wheat, whole wheat, Graham, rye, oat, maize, all-purpose, and soy flour. PN (0.006-0.41 mg/ 100 g), PM (0.010-0.044 mg/100 g), and PMP (0.01-0.024 mg/100 g) were found in lower amounts [26,27,30,31,43,50,51,72].…”