Vitamins B therapy in Regeneration of Peripheral Neuropathy Associated with Lipid Profile

Abstract

Objective: To investigate the role of serum lipid profile during the regeneration of experimental crush peripheral nerve injury to rats. Besides, to investigate which type of vitamin B therapy is better to accelerate the regeneration processes.Methods: Seventy-five male albino rats male, aged between 10 to 12 weeks were equally divided into 5 treated groups: B1, B6, B12, Tri-B complex and normal saline. Rats of therapeutic groups were administered according to the grouping and lasted to 45 consecutive days, and every 5 rats were sacrificed by euthanizing at 15th, 30th and 45th days. At dissection, sciatic nerve samples are taken from the site of crushed lesion and immersed in fixed formaldehyde 10%. 05 to 7 µm thick paraffin sections were stained by H&E and blood sample for lipid profile analysis.Results: The result showed no significant differences in serum lipid profile between therapeutic groups and control was found. Histological changes were shown on 15th days as, diffuse degeneration of the myelinated fibers with a marked vacuolization of some nerve fiber, numerous atrophic disoriented fibers with a large number of mononuclear phagocytes infiltration and degenerative debris. On 45th days there is a clear normal orientation of collagen fiber with very little debris and no vacuolization of the nerve. Morphometric measurements showed the myelin sheath thickness was increased significantly on the 30th and 45th days as compared with the control group. The result showed vitamin B12 is better in acceleration to nerve regeneration B12 groups showed better regenerative rate among other groups.Conclusion: no changes in serum lipid profile on experimental animals during regeneration processes. Vitamin B12 is better for enhancing the regenerative process.

The Impact of Supplements on Recovery After Peripheral Nerve Injury: A Review of the Literature

“…The deficiency of vitamin B12 can result in neurotoxicity and contribute to the development of subacute combined degeneration, a disorder of the CNS characterized by sensory deficits, motor weakness, paresthesia, and gait ataxia [ 79 ]. In addition, within the peripheral nervous system, there is evidence suggesting that B vitamins play a role in peripheral nerve repair following insult [ 80 ]. Four studies investigated the role of vitamin B12 after PNI using rat models; two used vitamin B12 in combination with other vitamins [ 81 , 82 ], and two investigated the role of vitamin B12 alone [ 80 , 83 ].…”

“…In addition, within the peripheral nervous system, there is evidence suggesting that B vitamins play a role in peripheral nerve repair following insult [ 80 ]. Four studies investigated the role of vitamin B12 after PNI using rat models; two used vitamin B12 in combination with other vitamins [ 81 , 82 ], and two investigated the role of vitamin B12 alone [ 80 , 83 ]. Combined B-vitamin administration improved the toe-spreading reflex [ 81 ].…”

The Role of Neurotropic B Vitamins in Nerve Regeneration

“…In addition, accelerated nerve regeneration by the combination of vitamins B1, B6, and B12 was suggested from other studies [63,64] which, however, were too small to show significant results. A recently published study investigated the effect of vitamin B therapy in the regeneration of experimental crush peripheral nerve injury to rats and appeared to contradict this finding and attributed vitamin B12 alone or in combination with B1 and B6 a stronger nerve-regenerating effect than vitamin B1 or B6 alone [65]. However, while the authors specified the doses of the individually administered vitamins (B1/B6/B12 180/180/1 mg/kg/day), they did not break it down for the combination (described as a total dose of 20 mg/kg/day).…”

The Role of Dietary Nutrients in Peripheral Nerve Regeneration

“…Al-Saaeed et al evaluated the rate of peripheral nerve regeneration in response to daily intramuscularly injection of different B vitamins (B1, B6, and B12). They found an increase in the number and density of regenerated myelinated fibers in the vitamin B-treated groups, in particular after treatment with vitamin B12, which was followed by vitamin B1 and vitamin B6 treatment [69,129]. A study by Ehmedah et al demonstrated a modulatory effect on the macrophage-SC interaction during the early stages of Wallerian degeneration after nerve injury.…”

“…Cobalamin is usually consumed in the form of the co-enzyme de-oxadenosylcobalamin or methylcobalamin. Several studies have highlighted a positive effect of methylcobalamin and vitamin B12 on the myelination process by increasing the expression of myelin basic protein in SCs [147], thus accelerating the myelination process [69,129]. Furthermore, when administered together with dexamethasone (an anti-inflammatory glucocorticoid often prescribed after peripheral nerve injury) after traumatic sciatic nerve injury in rats, vitamin B12 increases the number of SCs and myelinated nerve fibers as well as the diameter of regenerating axons.…”