Vitamins E and C prevent DNA double-strand breaks in peripheral lymphocytes exposed to radiations from iodine-131

Safaei, Mehdi; Jafarpour, Seyed Masoud; Mohseni, Mehran; Salimian, Morteza; Akbari, Hossein; Karami, Fateme; Aliasgharzadeh, Akbar; Farhood, Bagher

doi:10.4103/ijnm.ijnm_89_17

Cited by 8 publications

References 23 publications

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The interplay between vitamin C and thyroid

Farasati Far,

Behnoush,

Ghondaghsaz

et al. 2023

Endocrino Diabet & Metabol

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Vitamin C is involved in the maintenance of several body functions, and its role has been shown in several organs and systems. 1 Although its main form in the body is ascorbate, it acts as a co-substrate for several enzymes and antioxidants. 2,3 Its role as an antioxidant is well known and it is well known as reactive oxygen species (ROS) scavenger in neurons. 4,5 Its concentration in body fluids and tissues depends on intestinal absorption, cellular transport and excretion. 6 The thyroid gland, as one of the main sources of controlling

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The interplay between vitamin C and thyroid

Farasati Far,

Behnoush,

Ghondaghsaz

et al. 2023

Endocrino Diabet & Metabol

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Radiation-Induced Intestinal Injury: Molecular Mechanisms and Therapeutic Status

Gao,

Zhang,

Sun

et al. 2024

DNA and Cell Biology

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An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging

Harada

Hata

Fukuyama

et al. 2022

Preprint

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Phototoxicity is an important issue in fluorescence live imaging of light-sensitive cellular processes such as mitosis, especially in high spatiotemporal resolution microscopy that often requires high-intensity illumination. Among several approaches to reduce phototoxicity, the addition of antioxidants to the imaging media has been used as a simple and effective method. However, it remains unknown what are the optimal antioxidants that could prevent phototoxicity- induced defects during mitosis in fluorescence live cell microscopy. In this study, we analyzed the impact of phototoxicity on the mitotic progression in fluorescence live imaging of human diploid cells and performed a screen to identify the most efficient antioxidative agents that reduce it. Quantitative analysis shows that high amounts of light illumination cause various mitotic defects such as prolonged mitosis and delays of chromosome alignment and centrosome separation. Among several antioxidants known to reduce cellular phototoxicity, our screen reveals that ascorbic acid significantly alleviates these phototoxic effects in mitosis. Furthermore, we demonstrate that the addition of ascorbic acid to the imaging media enables fluorescence imaging of mitotic events at very high temporal resolution without obvious photodamage. Thus, this study provides a simple and practical method to effectively reduce the phototoxic effects on mitotic processes in fluorescence live cell imaging.

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Vitamins E and C prevent DNA double-strand breaks in peripheral lymphocytes exposed to radiations from iodine-131

Cited by 8 publications

References 23 publications

The interplay between vitamin C and thyroid

The interplay between vitamin C and thyroid

Radiation-Induced Intestinal Injury: Molecular Mechanisms and Therapeutic Status

An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging

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