Vitellogenesis and Yolk Proteins in Insects

Tufail, Muhammad; Takeda, Makio

doi:10.1016/b978-0-12-809633-8.20569-4

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…From the RPW fat body transcriptome, nearly 25 annotated genes were predicted to be involved in reproduction. Among these, Vg is one of the major gene which is highly expressed in RPW female fat bodies during reproductive phase and retain a substantial role in in oviparous organism's reproduction [9,29,[45][46][47][48][49][50][51]. The reproductive success of oviparous species depends on Vg production and accumulation in oocytes by membrane-bound receptors (the VgRs) via receptor-mediated endocytosis [8,[52][53][54][55].…”

Section: Plos Onementioning

confidence: 99%

De novo transcriptome analysis and identification of reproduction control genes from the red palm weevil Rhynchophorus ferrugineus

et al. 2021

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Recent attacks by the red palm weevil, Rhynchophorus ferrugineus (Olivier), have become a severe problem for palm species. In present work, fat body transcriptome of adult female red palm weevil was analyzed, focusing on the identification of reproduction control genes. Transcriptome study was completed by means of next-generation sequencing (NGS) using Illumina Hiseq 2000 sequencing system. A total of 105,938,182 raw reads, 102,645,544 clean reads, and 9,238,098,960 clean nucleotides with a guanine–cytosine content of 40.31%, were produced. The processed transcriptome data resulted in 43,789 unique transcripts (with mean lengths of 1,172 bp). It was found that 20% of total unique transcripts shared up to 80%–100% sequence identity with homologous species, mainly the mountain pine beetle Dendroctonus ponderosae (59.9%) and red flour beetle Tribolium castaneum (26.9%). Nearly 25 annotated genes were predicted to be involved in red palm weevil reproduction, including five vitellogenin (Vg) transcripts. Among the five Vg gene transcripts, one was highly expressed compared with the other four (FPKM values of 1.963, 1.471, 1.028, and 1.017, respectively), and the five Vg gene transcripts were designated as RfVg, RfVg-equivalent1, RfVg-equivalent2, RfVg-equivalent3, and RfVg-equivalent4, respectively. The high expression level of RfVg verified by RT-polymerase chain reaction analysis suggested that RfVg is the primary functional Vg gene in red palm weevil. A high similarity of RfVg with other Coleopterans was also reflected in a phylogenetic tree, where RfVg was placed within the clade of the order Coleoptera. Awareness of the major genes that play critical roles in reproduction and proliferation of red palm weevil is valuable to understand their reproduction mechanism at a molecular level. In addition, for future molecular studies, the NGS dataset obtained will be useful and will promote the exploration of biotech-based control strategies against red palm weevil, a primary pest of palm trees.

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Section: Plos Onementioning

confidence: 99%

De novo transcriptome analysis and identification of reproduction control genes from the red palm weevil Rhynchophorus ferrugineus

et al. 2021

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Impact of vitellogenin based dsRNA feeding on reproductive biology of red palm weevil, Rhynchophorus ferrugineus (Olivier), (Coleoptera: Dryophthoridae) under laboratory conditions

Rasool

Husain

Mehmood

et al. 2023

Journal of King Saud University - Science

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Transcriptome analysis of the almond moth, Cadra cautella, female abdominal tissues and identification of reproduction control genes

et al. 2019

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BackgroundThe almond moth, Cadra cautella is a destructive pest of stored food commodities including dates that causes severe economic losses for the farming community worldwide. To date, no genetic information related to the molecular mechanism/strategies of its reproduction is available. Thus, transcriptome analysis of C. cautella female abdominal tissues was performed via next-generation sequencing (NGS) to recognize the genes responsible for reproduction.ResultsThe NGS was performed with an Illumina Hiseq 2000 sequencer (Beijing Genomics Institute: BGI). From the transcriptome data, 9,804,804,120 nucleotides were generated and their assemblage resulted in 62,687 unigenes. The functional annotation analyses done by different databases, annotated, 27,836 unigenes in total. The transcriptome data of C. cautella female abdominal tissue was submitted to the National Center for Biotechnology Information (accession no: PRJNA484692). The transcriptome analysis yielded several genes responsible for C. cautella reproduction including six Vg gene transcripts. Among the six Vg gene transcripts, only one was highly expressed with 3234.95 FPKM value (fragments per kilobase per million mapped reads) that was much higher than that of the other five transcripts. Higher differences in the expression level of the six Vg transcripts were confirmed by running the RT-PCR using gene specific primers, where the expression was observed only in one transcript it was named as the CcVg.ConclusionsThis is the first study to explore C. cautella reproduction control genes and it might be supportive to explore the reproduction mechanism in this pest at the molecular level. The NGS based transcriptome pool is valuable to study the functional genomics and will support to design biotech-based management strategies for C. cautella.

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Vitellogenesis and Yolk Proteins in Insects

Cited by 3 publications

References 12 publications

De novo transcriptome analysis and identification of reproduction control genes from the red palm weevil Rhynchophorus ferrugineus

De novo transcriptome analysis and identification of reproduction control genes from the red palm weevil Rhynchophorus ferrugineus

Impact of vitellogenin based dsRNA feeding on reproductive biology of red palm weevil, Rhynchophorus ferrugineus (Olivier), (Coleoptera: Dryophthoridae) under laboratory conditions

Transcriptome analysis of the almond moth, Cadra cautella, female abdominal tissues and identification of reproduction control genes

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