Vitellogenesis in both sexes of gonochoristic mud shrimp, Upogebia major (Crustacea): Analyses of vitellogenin gene expression and vitellogenin processing

Kang, Bong Jung; Nanri, Takahiro; Lee, J.M.; Saitô, Hitoshi; Han, Cai; Hatakeyama, Masatsugu; Saigusa, Masahiro

doi:10.1016/j.cbpb.2007.12.003

Cited by 38 publications

(23 citation statements)

References 28 publications

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“…These results are in accord with those described for M. rosenbergii yolk protein processing. In a recent study on the mud shrimp, Upogebia major, belonging to the infraorder Thalassinidea of Decapoda, Kang et al [67] found three polypeptides in the oocytes. These subunits were found to be derived from a single long polypeptide translated from the Vg transcript in the hepatopancreas.…”

Section: Yolk Processingmentioning

confidence: 98%

Mechanisms and control of vitellogenesis in crustaceans

Subramoniam

2010

Fish Sci

165

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Crustaceans produce complex yolk proteins to meet the substrate and energy requirements of embryonic development. Early electron microscopic investigations point to a biphasic yolk synthesis, first within the ovary, followed by heterosynthesis at extra-ovarian sites. Recent advances in molecular techniques have enhanced our understanding of the genetic control of yolk synthesis in crustaceans. Amino acid sequencing of crustacean vitellogenin (Vg) has enabled the elucidation of the cDNA sequence associated with it, the identification of genes, and the examination of their expression patterns in different tissues. Yolk processing in crustaeans involves cleavage of the pro-Vg at consensus sites by subtilisin-like endoproteases within the hepatopancreas, hemolymph and oocytes. The structural elucidation of crustacean yolk proteins, as well as the comparison of amino acid sequences of vitellogenins from various crustacean species, has revealed molecular phylogenetic relationships not only among them but also with other large lipid transfer lipoproteins of disparate function. The combinatorial effects of eyestalk neuropeptides and a variety of trophic hormones achieve the hormonal coordination of molting and reproduction. Biogenic amines secreted by the central nervous system may also play an integrative role by stimulating neuropeptide secretion.

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Section: Yolk Processingmentioning

confidence: 98%

Mechanisms and control of vitellogenesis in crustaceans

Subramoniam

2010

Fish Sci

165

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“…It is generally accepted that the expression of Vg genes is upregulated in the hepatopancreas during the mature stages. The induction of Vg genes in the ovary has also been detected in various species, including P. monodon (Tiu et al, 2006), M. ensis (Tsang et al, 2003), Callinectes sapidus (Zmora et al, 2007), and Upogebia major (Kang et al, 2008). In our study, there was a 1972-fold induction of Pj-Vg2 transcripts in the mature ovary, whereas no significant increase in Pj-Vg1 was seen.…”

Section: Expression Analysismentioning

confidence: 52%

“…Most are from the commercially important penaeid shrimps in the suborder of Dendrobranchiata, including Marsupenaeus japonicus (Tsutsui et al, 2000), Penaeus monodon (Tiu et al, 2006), Litopenaeus vannamei (Raviv et al, 2006), Penaeus semisulcatus (Avarre et al, 2003), Fenneropenaeus chinensis (Xie et al, 2009), Fenneropenaeus merguiensis (Phiriyangkul and Utarabhand, 2006), and Pandalopsis japonica (Jeon et al, 2010). Others are from brachyuran and *Corresponding author: kimhw@pknu.ac.kr astacidean species in the suborder Pleocyemata, including Macrobrachium rosenbergii (Okuno et al, 2002), Pandalus hypsinotus (Tsutsui et al, 2004), Portunus trituberculatus (Yang et al, 2005), Cherax quadricarinatus (Abdu et al, 2002), Homarus americanus (Tiu et al, 2009), Upogebia major (Kang et al, 2008), Charybdis feriatus (Mak et al, 2005), Portunus trituberculatus (Yang et al, 2005), and Callinectes sapidus (Zmora et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Structural Similarity and Expression Differences of Two Pj-Vg Genes from the Pandalus Shrimp Pandalopsis japonica

Jeon¹,

Kim²,

Kim³

et al. 2011

Fisheries and aquatic sciences

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Vitellogenin (Vg) is the precursor of vitellin (Vn), which is the major yolk protein in nearly all oviparous species, including fish, amphibians, reptiles, and most invertebrates. It is one of the most important factors during reproduction, and numerous studies have shown that Vg genes are markers of the reproductive cycle and effecter genes induced by endocrine-disrupting chemicals (EDCs). Previously, we isolated two distinct cDNAs encoding vitellogenin homologs Pj-Vg1 and Pj-Vg2 from Pandalus shrimp Pandalopsis japonica. In this study, full-length genomic sequences of Pj-Vg1 and Pj-Vg2 were determined using a PCR-based genome walking strategy. Isolated Pj-Vg1 and Pj-Vg2 genes were 11,910 and 11,850 bp long, respectively. Both Pj-Vg genes had 15 exons and 14 introns, and the splicing sites were also the same, suggesting that they arose via gene duplication. The similar structural characteristics of decapod Vg genes suggest that they are all orthologs that evolved from the same ancestral gene. Analysis of Pj-Vg1 and Pj-Vg2 expression revealed that the relative copy numbers of Pj-Vg1 and Pj-Vg2 were similar in the hepatopancreas, whereas Pj-Vg2 transcripts were also detected in the ovary. Expression of both Pj-Vg genes was induced in hepatopancreas of mature individuals, whereas only Pj-Vg2 transcripts were upregulated in the ovaries from mature animals, suggesting that both Pj-Vgs are important for oocyte development. A strong positive correlation was found between Pj-Vg1 and Pj-Vg2 transcripts in the same individual, indicating they are under the same control mechanisms. Additionally, a positive correlation was found between ovarian and hepatopancreatic Pj-Vg2 transcripts, suggesting that its dual expression is regulated by similar physiological conditions. Knowledge of the similarities and differences between the two vitellogenin-like genes, Pj-Vg1 and Pj-Vg2, would help us to understand their roles in reproduction and other physiological effects.

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“…In several crustacean species, extraovarian Vtgs are enzymatically digested soon after their synthesis, and then transported via the hemolymph to the ovary where they are processed further into several subunits [6,[11][12][13][14][15]. As in the case of the blue crab, Callinectes sapidus [14], Vtg synthesized in the hepatopancreas is initially cleaved into ;78.5-and 207.3-kDa subunits, both of which are found in the hemolymph.…”

Section: Introductionmentioning

confidence: 98%

Characterization and Processing of Superoxide Dismutase-Fused Vitellogenin in the Diapause Embryo Formation: A Special Developmental Pathway in the Brine Shrimp, Artemia parthenogenetica1

Chen¹,

Chen²,

Yang³

et al. 2011

Biology of Reproduction

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To withstand environmental stress, Artemia release diapause cysts via an oviparous pathway instead of producing swimming nauplius larvae by the ovoviviparous pathway. Encased in such a cyst, the embryos at diapause can survive for many years. Vitellogenin (Vtg), the precursor of vitellins, the main yolk proteins, is crucial for embryonic development. This study compares vitellogenesis between oviparity and ovoviviparity, the two reproductive modes occurring in A. parthenogenetica. A Vtg gene was cloned, based on N-terminal amino acid sequence analysis, PCR amplification, and cDNA library construction and screening, and was found to consist of 6778 bp with a 6657 bp open reading frame encoding 2219 amino acids. From the deduced primary structure, Artemia vitellogenin (ArVtg) was found to possess six copies of the consensus cleavage site, R-X-X-R, and to contain a superoxide dismutase (SOD)-like domain at the N-terminus. This is an unusual finding for crustacean Vtg proteins, having been reported only in one previous crustacean, Daphnia magna. Using Northern blot analysis and in situ hybridization, ArVtg gene expression was observed at early stages of vitellogenesis in the connective tissue located in the cephalothorax, with trace expression in the ovary. Western blot analysis and several N-terminal sequences revealed that ArVtg was cleaved at each consensus cleavage site and that more than 10 subunits were formed during posttranslational processing in ovarian maturation. Of these, only the SOD-containing subunits (∼90 and 60 kDa) showed different profiles between the oviparous and ovoviviparous pathways. This suggests that these high concentration components have an important function for the encysted diapaused embryos during long-term cell-cycle arrest, which has remained unknown up until now.

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Vitellogenesis in both sexes of gonochoristic mud shrimp, Upogebia major (Crustacea): Analyses of vitellogenin gene expression and vitellogenin processing

Cited by 38 publications

References 28 publications

Mechanisms and control of vitellogenesis in crustaceans

Mechanisms and control of vitellogenesis in crustaceans

Structural Similarity and Expression Differences of Two Pj-Vg Genes from the Pandalus Shrimp Pandalopsis japonica

Characterization and Processing of Superoxide Dismutase-Fused Vitellogenin in the Diapause Embryo Formation: A Special Developmental Pathway in the Brine Shrimp, Artemia parthenogenetica1

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