Vitellogenesis in diapausing and mutant Drosophila melanogaster: further evidence for the relative roles of ecdysteroids and juvenile hormones

Richard, David; Jones, Jennifer M; Barbarito, Michael R; Cerula, Stacy; Detweiler, J.Paul; Fisher, S.; Brannigan, Dana M; Scheswohl, Danielle M

doi:10.1016/s0022-1910(01)00063-4

Cited by 97 publications

(59 citation statements)

References 30 publications

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“…Also, cpo is highly expressed in the ring gland (29), the primary endocrine structure in Drosophila; this is particularly interesting because diapause is under neuroendocrine control. We also identified a series of cpo ecdysone response elements, suggesting that the effects of cpo on diapause in D. melanogaster may be mediated by ecdysteroids (30). Loss-of-function cpo mutations are lethal, and partial loss-offunction mutations generate a variety of behavioral phenotypes (23) and neurological abnormalities (31).…”

Section: Discussionmentioning

confidence: 94%

An amino acid polymorphism in the couch potato gene forms the basis for climatic adaptation in Drosophila melanogaster

Schmidt

Zhu

Das

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

186

219

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Diapause is the classic adaptation to seasonality in arthropods, and its expression can result in extreme lifespan extension as well as enhanced resistance to environmental challenges. Little is known about the underlying evolutionary genetic architecture of diapause in any organism. Drosophila melanogaster exhibits a reproductive diapause that is variable within and among populations; the incidence of diapause increases with more temperate climates and has significant pleiotropic effects on a number of life history traits. Using quantitative trait mapping, we identified the RNAbinding protein encoding gene couch potato (cpo) as a major genetic locus determining diapause phenotype in D. melanogaster and independently confirmed this ability to impact diapause expression through genetic complementation mapping. By sequencing this gene in samples from natural populations we demonstrated through linkage association that variation for the diapause phenotype is caused by a single Lys/Ile substitution in one of the six cpo transcripts. Complementation analyses confirmed that the identified amino acid variants are functionally distinct with respect to diapause expression, and the polymorphism also shows geographic variation that closely mirrors the known latitudinal cline in diapause incidence. Our results suggest that a naturally occurring amino acid polymorphism results in the variable expression of a diapause syndrome that is associated with the seasonal persistence of this model organism in temperate habitats.cline ͉ diapause ͉ life history ͉ mapping ͉ tradeoff N atural populations encounter environmental stresses that diminish individual fitness, and these stresses are variable across space and time. It is predicted that the resulting natural selection often leads to a situation in which no genotype has the highest fitness across all environments, and polymorphism is maintained. This concept is pervasive in arguments about the evolution of life history variation and associated genetic tradeoffs (1). However, the expected molecular polymorphism associated with life history tradeoffs and adaptation remains elusive. Although we would expect these phenomena to be universal, the complexities of genetic dissection of such variation suggest that the best opportunity to identify the genetic basis for life history variation lies in the study of the genetic models in their natural populations (2).Drosophila melanogaster is a human commensal that has spread from areas of Sub-Saharan Africa to Europe and Asia, possibly over the last 5,000 to 16,000 years, and into the Western Hemisphere and Australia in the past several hundred years (3-5). This worldwide expansion from the tropics has required adaptation to the pronounced seasonality present in temperate habitats, and there are many examples of both single-gene polymorphism and quantitative trait variation that show geographic patterns associated with the transition from tropical to temperate climates in this species (6, 7). There also is good evidence that D. melanogaster ov...

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Section: Discussionmentioning

confidence: 94%

An amino acid polymorphism in the couch potato gene forms the basis for climatic adaptation in Drosophila melanogaster

Schmidt

Zhu

Das

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

186

219

View full text Add to dashboard Cite

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“…Exogenous application of juvenile hormone analogs also breaks diapause, and the diapause response is associated with a reduced rate of juvenile hormone synthesis in the corpora allata ). Studies by Richard et al (1998Richard et al ( , 2001) also implicated a role for ecdysteroids in mediating vitello- genesis and the diapause response and further supported that D. melanogaster diapause is under neuroendocrine control. The expression of diapause was shown to vary both among laboratory strains and natural populations (Williams and Sokolowski 1993).…”

Section: Variance For the Diapause Phenotypementioning

confidence: 91%

Genetic Variance for Diapause Expression and Associated Life Histories in Drosophila Melanogaster

2005

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Abstract. The dipteran Drosophila melanogaster can express a form of reproductive quiescence or diapause when exposed to low temperature and shortened photoperiod. Among natural populations in the eastern United States, the frequency of lines that express reproductive diapause in the laboratory varies substantially and predictably with latitudinal origin. The goals of the present study were twofold: (1) to examine the impact of genetic variance for diapause expression on multiple traits associated with organismal fitness; and (2) to evaluate the potential for fitness trade-offs between diapause and nondiapause phenotypes that may result in the observed cline. Even prior to diapause entry or expression, inbred lines that express and do not express reproductive diapause in laboratory assays were constitutively distinct for life span, age-specific mortality rates, fecundity profiles, resistance to cold and starvation stress, lipid content, development time, and egg-to-adult viability. Furthermore, estimates of genetic correlations based on line means revealed significant differentiation for genetic variance/covariance matrices between diapause and nondiapause lines. The data indicate the potential for life-history trade-offs associated with variation for the diapause phenotype. The observed cline in diapause incidence in the eastern United States may be generated by these tradeoffs and the associated spatial and/or temporal variation in relative fitness of these two phenotypes in natural populations.

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“…During the reproductive phase of the fruit-fly, JH controls the early synthesis of yolk proteins (YPs) by the fat bodies and the ovarian follicle cells, and YPs are taken up by the developing egg chambers. JH also induces the ovaries to produce 20-hydroxyecdysone (20-OH Ecd), which sustains late vitellogenesis, by inducing the synthesis and uptake of YPs by the fat bodies [72][73][74][75]. However, an excess of 20-OH Ecd induces apoptosis in nurse cells of stage 9 egg chambers, during early vitellogenesis.…”

Section: Larval and Adult Diapause Are Regulated By Jh And Ecdmentioning

confidence: 99%

The hormonal and circadian basis for insect photoperiodic timing

2011

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Edited by Martha Merrow and Michael BrunnerKeywords: Insects Photoperiodism Diapause Endocrine axis Circadian clock Clock genes a b s t r a c t Daylength perception in temperate zones is a critical feature of insect life histories, and leads to developmental changes for resisting unfavourable seasons. The role of the neuroendocrine axis in the photoperiodic response of insects is discussed in relation to the key organs and molecules that are involved. We also discuss the controversial issue of the possible involvement of the circadian clock in photoperiodicity. Drosophila melanogaster has a shallow photoperiodic response that leads to reproductive arrest in adults, yet the unrivalled molecular genetic toolkit available for this model insect should allow the systematic molecular and neurobiological dissection of this complex phenotype.

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Vitellogenesis in diapausing and mutant Drosophila melanogaster: further evidence for the relative roles of ecdysteroids and juvenile hormones

Cited by 97 publications

References 30 publications

An amino acid polymorphism in the couch potato gene forms the basis for climatic adaptation in Drosophila melanogaster

An amino acid polymorphism in the couch potato gene forms the basis for climatic adaptation in Drosophila melanogaster

Genetic Variance for Diapause Expression and Associated Life Histories in Drosophila Melanogaster

The hormonal and circadian basis for insect photoperiodic timing

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