Vitellogenin content in European eel (Anguilla anguilla) in Flanders, Belgium

Versonnen, Bram; Goemans, Geert; Belpaire, Claude; Janssen, Colin R.

doi:10.1016/j.envpol.2003.09.013

Cited by 39 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The utilisation of the ALP assay was also shown to be insensitive in the present study. Using fish exposed to 17α -ethinylestradiol both Verslycke et al (2002) and Versonnen et al (2004) showed that plasma ALP and Ca assays have the same sensitivity to oestrogen effects. According to many authors this is largely because each Ca ion is bound to the phosphate groups on the VTG molecule (Gosh and Thomas, 1995;Fuentes et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The two plasma biomarkers were utilised as indirect measures of VTG circulating in the blood, based on numerous fish studies indicating a significant correlation between VTG and Ca, phosphate, Mg and Zn, respectively (Verslycke et al, 2002;Versonnen et al, 2004;Lv et al, 2006). In the presence of EDC's, high levels of the female protein VTG can unnaturally accumulate in male and juvenile fish (Tsai and Wang, 2000;Guerreiro et al, 2002;Gillespie and de Peyster, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Although studies have shown the induction of VTG in C. gariepinus in response to EDC's (Braathen et al, 2009), no known studies have utilised ALP, Ca and Mg as indirect measures in this species. Studies using Anguilla anguilla and Misgurnus anguillicaudatus, however, showed that ALP, Ca, Mg and Zn are all present on the VTG molecule in high abundances and can, therefore, be linked to VTG concentrations (Verslycke et al, 2002;Versonnen, et al, 2004;Lv et al, 2006). In order to identify higher levels of effects, the gonads were assessed for negative alterations in the gonad mass and cellular morphology, using gonad-somatic index (GSI), interpreted with analysis of covariance (ANCOVA) manipulated gonad mass and histologically assessed for intersex (female oocytes in male gonads) (Kime, 1998;Jobling and Tyler, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The lack of endocrine disrupting effects in catfish (Clarias gariepinus) from a DDT sprayed area

Brink

Vuren

Bornman

2012

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

The exposure and response of the catfish, Clarias gariepinus, was studied in male specimens collected in the vicinity of a DDT spraying programme to control malaria. Two sites were situated in the DDT sprayed areas and one site upstream from exposed areas, used as a reference site. The collected specimens were analysed for DDT bioaccumulation and the extent of associated effects. The concentration of all DDT metabolites including p,p'-and o,p'-forms of DDT, DDE and DDD, were measured in the adipose tissue, whilst the effects were measured using a range of biomarkers. This included assessing the effectiveness of plasma calcium, magnesium, zinc and alkali-labile phosphates (ALPs) as indirect measures of vitellogenin (VTG). Gonad condition was determined by calculating the gonadosomatic index (GSI) for each individual and comparing it with the gonad mass that were adjusted with Analysis of Covariance (ANCOVA). The presence of intersex in gonads was identified and the overall body condition determined using the condition factor (CF). Overall, none of the biomarkers showed significant change in the presence of high levels of DDT nor lindane, dieldrin and endosulfan II. Subtle responses in the plasma concentrations of calcium, ALP and gonad condition were evident in the catfish where DDT concentrations were highest, whilst no effects related to intersex and body condition were evident. Overall this study highlighted the tolerance of C. gariepinus to DDT contamination, the practical implications of using biomarkers in developing countries, and the need for further research into developing biomarkers for much needed biomonitoring programmes in areas where malarial control programmes continue to use DDT.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The lack of endocrine disrupting effects in catfish (Clarias gariepinus) from a DDT sprayed area

Brink

Vuren

Bornman

2012

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

show abstract

“…No one knows exactly where the European eels (Anguilla anguilla) spawning ground is (Lokman & Young 2000;Ringuet et al 2002). The smallest larvae found in the Sargasso Sea, south of Bermuda, suggest that spawning of the A. anguilla as well as that of the American eel (Anguilla rostrata) occurs nearby (Wirth & Bernatchez 2001Mank & Avise 2003;Versonnen et al 2004;Dannewitz et al 2005;Palstra et al 2006). Meanwhile, the Japanese eels (Anguilla japonica) spawning ground appears to be in the west of the Mariana Islands in the North Pacific (Katoh & Kobayashi 2001;Tsukamoto & Arai 2001;Ringuet et al 2002;Tseng et al 2003;Tsukamoto 2006).…”

Section: Introductionmentioning

confidence: 99%

INVESTIGATION OF GENETIC VARIABILITY IN THE EUROPEAN EEL (ANGUILLA ANGUILLA(L.)) IN LITHUANIA USING MICROSATELLITE DNA MARKERS

Sruoga

Butkauskas

Ragauskas

et al. 2007

Acta Zoologica Lituanica

View full text Add to dashboard Cite

Abstract. To find out if native and introduced European eel (Anguilla anguilla) populations differ from one another genetically, we used five microsatellite markers and compared naturally recruited and introduced European eel populations at four different locations. Samples of naturally recruited eels were taken from the Baltic Sea and the Curonian Lagoon, while introduced eels were sampled from Lakes Dringis and Siesartis. Results of the study do not support the hypothesis on panmixia in the European eel, since low but significant genetic differentiation (F ST = 0.0238, p = 0.0148) was found to exist among introduced eel groups. Significant genetic differentiation (F ST = 0.0228, p = 0.0281) among the Baltic Sea, the Curonian Lagoon, Lake Dringis and Lake Siesartis populations was ascertained too.

show abstract

“…[8] Elevated plasma Vtg was also found in eels below the Paris outfall. [9] Because eels may spend up to 20 years in the river before their spawning, migration-consequent reproductive disruption may account for some of the decrease in the European eel population. [9] The flounder, as a bottom-dwelling estuarine species, also provides a useful biomonitor of effluents in the rivers, which may potentially affect marine fish stocks.…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin assay by enzyme-linked immunosorbant assay as a biomarker of endocrine disruptor chemicals pollution

Ebrahimi

2005

Indian J Occup Environ Med

View full text Add to dashboard Cite

There is increasing evidence that many xenobiotic chemicals (called as endocrine disruptor chemicals, EDCs) through interfering with the endocrine system, have the capability to induce developmental and reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (Vtg) has proved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs, especially the estrogenic compounds. Work is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals, and in the focus of this development are the fish test species common carp (Cyprinus carpio). In this study, we have developed quantitative enzyme-linked immunosorbent assays (ELISA) for VTS in common carp. The working range of the ELISA was 11.25 -2000 ng/ml (75 -25% specific binding/maximum antibody binding [B/B0]) with a 50% B/B0 intra and interassay variation of 3.9% (n = 10) and 12.5% (n = 30), respectively. This ELISA is capable of detecting Vtg as low as 6 ng/ml, and can accurately detect Vtg in even 10 ml of plasma. The ELISA was applied to measurement of Vtg production by male carp (C. carpio, Cyprinidae) fish exposure to ethynylestradiol. The results showed that the amount of Vtg produced in plasma of exposed fish increased in logarithmic order comparing to the control group and the ELISA described here could be used as an indicator of water pollution to estrogenic pollutants.

show abstract

Vitellogenin content in European eel (Anguilla anguilla) in Flanders, Belgium

Cited by 39 publications

References 30 publications

The lack of endocrine disrupting effects in catfish (Clarias gariepinus) from a DDT sprayed area

The lack of endocrine disrupting effects in catfish (Clarias gariepinus) from a DDT sprayed area

INVESTIGATION OF GENETIC VARIABILITY IN THE EUROPEAN EEL (ANGUILLA ANGUILLA(L.)) IN LITHUANIA USING MICROSATELLITE DNA MARKERS

Vitellogenin assay by enzyme-linked immunosorbant assay as a biomarker of endocrine disruptor chemicals pollution

Contact Info

Product

Resources

About