Vitellogenin Transport and Yolk Formation in the Quail Ovary

Ito, Yasushi; Kihara, M; Nakamura, Etsuko; Yonezawa, Satoshi; Yoshizaki, Norio

doi:10.2108/zsj.20.717

Cited by 30 publications

(31 citation statements)

References 19 publications

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“…Then, the egg yolk proteins may undergo digestion by the intestinal enzymes and/or the endogenous yolk proteases. Our suggestion depends on the previous studies in quail which showed the digestion of egg proteins inside the egg yolk by the proteolytic enzymes that are activated at the second half of incubation (Yoshizaki et al 2002(Yoshizaki et al , 2004Ito et al 2003). After their digestion, the yolk proteins are probably taken up via endocytosis by the endodermal cells of the yolk sac.…”

Section: Discussionmentioning

confidence: 82%

“…The two main sources of egg nutrients are the egg white and yolk. How the egg white is ultimately transferred into the yolk, and then taken up by the developing embryo, has been well investigated in the chicken (Saito et al 1965;Saito & Martin 1966;Baintner & Fehér 1974;Sugimoto et al 1984Sugimoto et al , 1989Sugimoto et al , 1999Burley & Vadehra 1989) and quail (Yoshizaki et al 2002(Yoshizaki et al , 2004Ito et al 2003); however, it is largely unexplored in the turkey. In the present study, we took a step toward exploring the route(s) of egg white transfer into the yolk in fertilized turkey eggs.…”

Section: Discussionmentioning

confidence: 99%

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Transfer of egg white proteins with reference to lysozyme during the development ofMeleagris gallopavo(Galliformes: Phasianidae) embryos

Shbailat

Safi

2015

Italian Journal of Zoology

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The egg white and egg yolk are the two main sources of nutrients for the developing avian embryo. The egg white should be transferred into the yolk in order to be consumed by the embryo. How the egg white ultimately reaches the egg yolk is largely unexplored in the turkey Meleagris gallopavo. Here, we explored the routes of egg white transfer in fertilized turkey eggs. Initially, we tested the electrophoretic pattern of the proteins in different egg compartments throughout development. Then, we used lysozyme as a reference protein to follow the egg white transfer, and we measured its activity using Micrococcus lysodeikticus as a substrate. We found that several presumptive egg white protein bands appeared in the different egg compartments. Also, the electrophoretic patterns in the intestinal fluid and thick yolk were marked by the disappearance of large bands and the appearance of small ones at late developmental stages. Moreover, we detected a chronological appearance of lysozyme activity in the different egg compartments. The activity appeared in the extraembryonic and amniotic fluids on day 15, in the intestinal fluid on day 16 and in the thick yolk on day 17, and it increased in general with the progress of development. Our results suggest that the main route of egg white transfer is albumen sac -extraembryonic cavity -amniotic cavity -intestinal lumen -egg yolk. Furthermore, the transferred egg white proteins seem to undergo digestion in the intestinal lumen and egg yolk at late developmental stages.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Transfer of egg white proteins with reference to lysozyme during the development ofMeleagris gallopavo(Galliformes: Phasianidae) embryos

Shbailat

Safi

2015

Italian Journal of Zoology

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“…During this period, the limiting membranes of the yolk spheres were lost (Yoshizaki et al 2004). As a consequence, the yolk granules and the aspartic protease cathepsin D, initially found inside the spheres, became mixed with the surrounding medium, which was high in solute concentration (Ito et al 2003;Yoshizaki et al 2004). Under these conditions, cathepsin D started the digestion of yolk granules at a high rate; however, the rate of decrease in yolk-granule weight became slow after day 12 (Yoshizaki et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…This transfer was followed by a rapid increase in the activity of neutral and serine proteases in the egg yolk (Sugimoto & Yamada 1986). In contrast, in quail, the activation of acidic proteases preceded the major transfer of egg white into the yolk, which occurred after day 12 (Yoshizaki et al 2002(Yoshizaki et al , 2004Ito et al 2003). The activated proteases appeared to digest the egg white and yolk proteins before their transfer into the growing embryo (Sugimoto & Yamada 1986;Yoshizaki et al 2002Yoshizaki et al , 2004.…”

Section: Introductionmentioning

confidence: 99%

“…The egg yolk consists of nearly 50% water, 33% fat, 15% protein, less than 1% carbohydrate, and the rest of various minerals and vitamins (Shenstone 1968;Vieira 2007). Egg white is ultimately transferred into the yolk before being consumed by the developing embryo (Saito et al 1965;Saito & Martin 1966;Baintner & Fehér 1974;Sugimoto et al 1978Sugimoto et al , 1984Sugimoto et al , 1989Sugimoto et al , 1999Burley & Vadehra 1989;Yoshizaki et al 2002Yoshizaki et al , 2004Ito et al 2003;Shbailat & Safi 2015). In chicken, the major transfer of egg white into the yolk through the amniotic sac and intestinal lumen of the embryo occurred after day 12 (McIndoe 1960;Saito et al 1965;Saito & Martin 1966;Carinci & Manzoli-Guidotti 1968;Baintner & Fehér 1974;Burley & Vadehra 1989;Sugimoto et al 1989).…”

Section: Introductionmentioning

confidence: 99%

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Protease activity in the egg yolk during the development of Meleagris gallopavo (Galliformes: Phasianidae) embryos

Shbailat

Qanadilo

Alsoubani

2016

Italian Journal of Zoology

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The egg white and egg yolk are the two main sources of nutrients for the developing avian embryo. Egg white ultimately reaches egg yolk before being consumed by the embryo. Previously, in the turkey Meleagris gallopavo, we showed that the major egg white transfer into the yolk through the amnion and intestinal lumen of the embryo started on day 17 and became obvious on day 19. We also suggested that the transferred egg white and endogenous yolk proteins undergo digestion at late developmental stages. However, the protease activity throughout development and the type of activated proteases are completely unexplored in the yolk of turkey eggs. Here, we measured the general proteolytic activity in the egg yolk during different developmental stages using casein as a substrate. Furthermore, we determined the type of activated proteases by employing different types of protease inhibitors. Protease activity in the egg yolk was basal throughout development until day 19 when activity significantly increased, remaining high thereafter. Moreover, acidic aspartic protease(s) were primarily activated in the yolk. Our results suggest that the transferred egg white and endogenous yolk proteins are mostly degraded by the aspartic proteases that become highly activated late in development.

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Dietary yolk supplements and rate of yolk deposition do not influence sex ratios in Japanese Quail

2023

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Birds appear to have the ability to bias offspring sex ratios in response to environmental and social conditions. The mechanisms responsible remain unknown, however one previous study indicated a link between rates of ovarian follicle growth and the sexes of resulting eggs. This could indicate that either follicles destined to retain male or female follicles grow at different rates, or the rate of ovarian follicle growth determines the sex chromosome that will be retained to influence the sex of the resulting offspring. We tested for evidence of both possibilities by staining yolk rings that are indicative of daily growth. First, we tested for a correlation between the number of yolk rings and the sexes of the resulting germinal discs collected from each egg, and second, we tested whether experimentally decreasing follicle growth rates with a dietary yolk supplement impacts the sexes of the resulting germinal discs. There was no significant correlation between the number of yolk rings and the sexes of the resulting embryos, and decreasing follicle growth rates did not impact sexes of resulting germinal discs. These results indicate that offspring sex is not related to the rate of ovarian follicle growth in quail.

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Vitellogenin Transport and Yolk Formation in the Quail Ovary

Cited by 30 publications

References 19 publications

Transfer of egg white proteins with reference to lysozyme during the development ofMeleagris gallopavo(Galliformes: Phasianidae) embryos

Transfer of egg white proteins with reference to lysozyme during the development ofMeleagris gallopavo(Galliformes: Phasianidae) embryos

Protease activity in the egg yolk during the development of Meleagris gallopavo (Galliformes: Phasianidae) embryos

Dietary yolk supplements and rate of yolk deposition do not influence sex ratios in Japanese Quail

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