Vitellogenin2: spermatozoon specificity and immunoprotection in mud crabs

Yang, Yanan; Zheng, Biqi; Bao, Chenchang; Huang, Huiyang; Ye, Haihui

doi:10.1530/rep-16-0188

Cited by 18 publications

(10 citation statements)

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“…The mud crab, S. paramamosain, is an annual animal and reproduces once in its lifetime. The ovarian development goes through three main stages: pre-vitellogenic stage, earlyvitellogenic stage, and late-vitellogenic stage (30,31). In this study, Sp-CFSH expression was also found to be different in females during ovarian development.…”

Section: Discussionmentioning

confidence: 50%

“…In addition, the eyestalk ganglion of female crabs (n = 8) were collected to analyze the Sp-CFSH expression. Ovarian development was distinguished according to the morphological characteristics and confirmed by histological observation (30,31). All the animals used in this study have been approved by the Animal Ethics Committee of Xiamen University.…”

Section: Animal Sourcesmentioning

confidence: 99%

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DNA Methylation Inhibits the Expression of CFSH in Mud Crab

et al. 2020

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Crustacean female sex hormone (CFSH) is a key regulator of crustacean sex differentiation. The expression of Sp-CFSH in the mud crab Scylla paramamosain showed a tissue-specific and gender-variant pattern. To explore the role of DNA methylation in Sp-CFSH expression, the 5 ′-flanking region of Sp-CFSH was cloned, and one CpG island containing 12 CpG sites was found. Results of sodium bisulfite sequencing and methylated DNA immunoprecipitation showed that CpG island methylation was stable in the eyestalk ganglion during ovarian development of the females, which was significantly lower than that in the muscle of the females and in the eyestalk ganglion of the males. Such results suggested that the involvement of DNA methylation in regulating Sp-CFSH expression followed an eyestalk ganglion-specific and gender-variant pattern. The analysis of CpG dinucleotide site methylation and activity of the site-directed mutation (SDM) reporter vector further demonstrated that methylation inhibited Sp-CFSH expression by blocking the binding of transcription factor Sp1. The finding suggested for the first time the involvement of CpG methylation in the regulation of Sp-CFSH expression.

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Section: Discussionmentioning

confidence: 50%

Section: Animal Sourcesmentioning

confidence: 99%

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

et al. 2020

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“…may have an antimicrobial function or immune-related functions (18)(19)(20)(21). For example, in the crab, a Vg2 cDNA was detected in the hemocyte and the function of the Vg2 appeared to be immunity-related (17,18).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Gene Sequence Divergence, Expression Dynamics, and Endocrine Regulation of the Vitellogenin Gene Family in the Whiteleg Shrimp Litopenaeus vannamei

Wang

Zhou

et al. 2020

Front. Endocrinol.

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In this report, we studied the vitellogenin gene family in the whiteleg shrimp Litopenaeus vannamei by transcriptomics, bioinformatics, and molecular biology methods. At least three moderately homologous vitellogenin (Vg) genes (i.e. LvVg1, LvVg2, and LvVg3) were identified in the genome. The deduced LvVg proteins consisted of a vitellogenin_N domain, a DUF1943 domain, and a VWD domain typical of most vitellogenins from oviparous animals. LvVg1 was the most abundant Vg expressed in the hepatopancreas and ovary of maturing females. Furthermore, multiple isoforms of LvVg1 were evolved presumably due to the need for rapid Vg production during the rapid phase of vitellogenesis. LvVg transcripts were detected in different larval stages, juveniles, and subadults. During the non-reproductive cycle, LvVg expression in the hepatopancreas peaked at the intermolt stages. During the female vitellogenesis cycle, a two-phase expression pattern of LvVg1 gene was observed in the hepatopancreas and ovary. Moreover, the eyestalk optic nerve, brain, and thoracic ganglion consisted of factors that differentially regulated the expression of the three Vg genes. In addition to their reproduction-related roles, Vg may also be involved in growth and molt-related processes. Phylogenetic analysis revealed the early expansion and separation of these Vg genes, and it is most likely correlated with the expansion of Vg’s function. In conclusion, the evolution of multiple LvVg1 isoforms and the acquisition of different Vg genes (i.e. LvVg2 and LvVg3) may occur universally in most decapods. Full information on the total number of Vg genes and precise knowledge on the expression pattern and endocrine regulation of each Vg during all life cycle stages are crucial for us to understand the roles of this emerging gene family in the control of shrimp reproduction and other non-reproductive processes.

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“…Moreover, Vg can protect some species from oxidative stress, such as the honey bee, nematodes ( Caenorhabditis elegans ), and fish ( Anguilla japonica ) (Ando and Yanagida, 1999; Nakamura et al, 1999; Seehuus et al, 2006). In addition, Vg plays important roles during immunity in amphioxus (Zhang et al, 2005), fish (Li et al, 2008, 2009; Liu et al, 2009; Garcia et al, 2010), bay scallop (Wu et al, 2015), mud crab (Yang et al, 2016), and noble scallop (Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin and Vitellogenin-Like Genes in the Brown Planthopper

et al. 2019

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Vitellogenin (Vg) is precursor of vitellin. Here, we identified a Vg (NlVg) and two Vg-likes (NlVg-like1 and NlVg-like2) in the brown planthopper, Nilaparvata lugens. Phylogenetic analyses showed that NlVg-like1 and NlVg-like2 are not clustered with the conventional insect Vgs associated with vitellogenesis. Temporo-spatial expression analyses showed that the NlVg and NlVg-like2 transcript levels increased significantly 24 h after emergence and were primarily expressed in female adults. However, NlVg-like1 was expressed during all stages, and in both genders. Tissue-specific analyses showed that all three genes were most highly expressed in the fat body. The injection of double-stranded RNA targeting NlVg showed that NlVg is essential not only for oocyte development but also for nymph development. The knockdown of NlVg-like1 in female adults resulted in failure to hatch or death before eggshell emergence in 18% of offspring embryos, suggesting that NlVg-like1 plays an important role during late embryogenesis. Approximately 65% of eggs laid by females that were treated with double-stranded RNA targeting NlVg-like2 failed to hatch, indicating that NlVg-like2 plays a role in nutrition absorption during oocyte, or embryonic development. Our results illustrate the structural and functional differences among the Vg and Vg-like genes and provide potential targets for RNA-interference-based insect pest management strategies.

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Vitellogenin2: spermatozoon specificity and immunoprotection in mud crabs

Cited by 18 publications

References 50 publications

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

Investigation of Gene Sequence Divergence, Expression Dynamics, and Endocrine Regulation of the Vitellogenin Gene Family in the Whiteleg Shrimp Litopenaeus vannamei

Vitellogenin and Vitellogenin-Like Genes in the Brown Planthopper

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