Vitex Rotundifolia Fractions Induced Apoptosis in Human Breast Cancer T-47D Cell Line via Activation of Extrinsic and Intrinsic Pathway

Abstract

Breast cancer is the most frequently diagnosed cancer and is common cause of death in women both in developed and developing countries. Different therapies are used to block breast cancer progression, however in developing countries, survival rates for breast cancer are still poor and it accounts for 22.9% of all cancers in women (Guerrero et al., 2017; Mohankumar et al., 2013). Current therapies for cancer such as chemotherapy, radiotherapy or immunotherapy primarily act by causing cell death in cancer cells. Therefore, inability to exert

Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA

“…Cells were culture in 5% CO 2 at 37°C. [ 11 12 13 14 ] Cell lines were grown in 25 cm 3 and 75 cm 3 tissue culture flasks in a humidified atmosphere, containing 5% CO 2 and 95% air, at 37°C. The cell culture procedure and guideline followed by Ethical Committee of the Faculty of Medicine and Health Sciences, UPM.…”

Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway

“…The assay was performed according to a previously described method. [ 20 23 ] The breast cancer cell line was treated with active partition extracts of B. gymnorrhiza at a concentration of IC 50 at 72 h and incubated for several time points (0 h–36 h) at 37°C. The reading of each sample was measured using a luminometer at Optical density (OD) of 490 nm.…”

The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

“…20 Meanwhile, caspase-3 is the part of protease enzyme family which contributes essentially and fundamentally in the apoptosis and caspase 8 is the initiator to cleave Atg3 during receptor-mediated cell death and induces apoptosis. 19 As the cell targets, osteosarcoma stem cells (OS-SCs) can be generated from the patient's osteosarcoma tissues. Besides that, not only that osteosarcoma tissues are the resource of cancer stem but these tissues can also produce the extracted osteosarcoma associated stromal cells which are non-tumoral mesenchymal stem cell-like cells.…”

“…The biomarker which can be used to detect the apoptosis via extrinsic pathway along with annexin V, caspase-3 and caspase-8. 19 Known as one of cellular proteins within the annexin group, annexin V is often employed to identified an apoptosis by tying up phosphatidylserine outside the plasma membrane. Annexin V which binds the uppermost layer of the cellular membrane’s phosphatidylserine could detect the process of cell damage or apoptosis.…”

“… 20 Meanwhile, caspase-3 is the part of protease enzyme family which contributes essentially and fundamentally in the apoptosis and caspase 8 is the initiator to cleave Atg3 during receptor-mediated cell death and induces apoptosis. 19 …”