Vitreous levels of vascular endothelial growth factor, stromal cell–derived factor-1α, and angiopoietin-like protein 2 in patients with active proliferative diabetic retinopathy

Keles, Ali; Sönmez, Kenan; Erol, Yasemin Özdamar; Ayyıldız, Sema Nur; Öğüş, Ersin

doi:10.1007/s00417-020-04889-0

Cited by 9 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even Chen et al ( 30 ) found a significant positive correlation between SDF-1 and VEGF in the vitreous of proliferative diabetic retinopathy patients. However, Keles et al ( 31 ) observed no relationship between SDF-1 and VEGF in the vitreous of active proliferative diabetic retinopathy patients. Further, the contribution of SDF-1 has been shown in the pathogenesis of retinopathy of prematurity characterized by angiogenesis ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Aqueous humor levels of vascular endothelial growth factor and stromal cell-derived factor-1α in age-related macular degeneration

Keles¹

2021

Beyoglu Eye J

Self Cite

View full text Add to dashboard Cite

Objectives: This study investigated the contribution of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) angiogenic mediators in eyes with age-related macular degeneration (AMD). Methods: Aqueous humor specimens taken during cataract surgery in 7 cases of intermediate stage (nonexudative) AMD and 7 cases of late stage (exudative) AMD were evaluated using chemiluminescent immunoassay testing in this prospective case-control study. Mediator levels were compared with the normal reference values of 7 patients without any disease other than cataract. Results: The groups were similar in terms of age and gender (p>0.05). The aqueous humor levels of VEGF in both the intermediate AMD (median: 224.3 pg/mL, range: 44.8–380.4 pg/mL) and late-stage AMD (median: 108.7 pg/mL, range: 61.9-223.5 pg/mL) patients were similar to those of the control group (median: 121.1 pg/mL, range: 24.9–156.6 pg/mL) (p=0.256). Moreover, there was no significant difference in the SDF-1α concentrations between the intermediate AMD (median: 160.9 pg/mL, range 130–166.3 pg/mL), late AMD (median: 161 pg/mL, range: 154.1.9–171.6 pg/mL), and control group values (median: 161 pg/mL, range: 155.2–219 pg/mL) (p=0.763). Conclusion: The aqueous humor levels of VEGF and SDF-1α were within the normal range in patients with intermediate and late-stage AMD.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Studies on diabetic retinopathy characterized by neovascularization due to retinal ischemia have shown that SDF-1 plays an important role ( 29 - 31 ). Even Chen et al ( 30 ) found a significant positive correlation between SDF-1 and VEGF in the vitreous of proliferative diabetic retinopathy patients.…”

Section: Discussionmentioning

confidence: 99%

Aqueous humor levels of vascular endothelial growth factor and stromal cell-derived factor-1α in age-related macular degeneration

Keles¹

2021

Beyoglu Eye J

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, in FMV tissues, local cells were mainly hyalocytes, myofibroblasts, fibroblasts, and an abundance of glial cells migrated from the posterior vitreous cortex or the superficial retina, rather than leaking from the capillaries or micro vessels in retina or FVMs [4,24] . VEGF concentration in vitreous fluid is a classic marker for evaluating angiogenesis activity in PDR [25] . MVD, a quantitative index of neovascularization used to evaluate angiogenesis activity and tumor intravasation [26] , has also been applied to assess FVM activity in PDR [27] .…”

Section: Discussionmentioning

confidence: 99%

Effect of intravitreal ranibizumab on fibrovascular membranes in patients with proliferative diabetic retinopathy

Liang¹,

Li²,

Yang³

et al. 2022

Int J Ophthalmol

View full text Add to dashboard Cite

AIM: To assess the effects of intravitreal ranibizumab (IVR) on angiogenesis and glial activity of the fibrovascular membrane (FVM) in patients with proliferative diabetic retinopathy (PDR). METHODS: Forty-two eyes from 42 patients with PDR requiring vitrectomy were included and divided into two groups: control group (n=16) did not receive IVR, while IVR group (n=26) underwent IVR 5d before vitrectomy. FVM specimens were collected by the same surgeon during the interventions. Histopathological morphology was examined by hematoxylin-eosin (H-E) staining and cell densities in the FVM was assessed. Microvessels were outlined by immunohistochemical staining of CD31 and microvessel density (MVD) assessed as an index of FVM angiogenesis. Dual-color immunofluorescence staining, and confocal microscopy was used to detect co-localization and relative expression levels of glial fibrillary acidic protein (GFAP) and α-smooth muscle actin (α-SMA) as markers of glial-mesenchymal transition (GMT). The GMT index (GI; ratio of relative GFAP/α-SMA expression) was used to semi-quantify the degree of GMT or glial activity of FVMs. RESULTS: H-E staining showed similar vascularization in both groups, with microvessels and scattered stromal cells in the matrix. Infiltrated cell densities did not differ significantly between the two groups (P>0.05). The MVD of the IVR group (130.62±15.46/mm2) was significantly lower than that of the controls (142.25±19.16/mm2, P<0.05). In both groups, all sections were strongly immunostained for GFAP and α-SMA. The Pearson's correlation coefficients (PCC) of intensity of automated pixel count of two markers indicated GFAP and α-SMA co-stained well and GMT participated in the remolding of FVMs in PDR. The mean relative GFAP expression in the IVR group was significantly lower, whereas that of α-SMA was significantly higher than in controls (P<0.05). GI in the IVR group (1.10±0.10) was significantly lower than in the controls (1.21±0.12, P<0.05). CONCLUSION: IVR can reduce angiogenesis, glial activity of FVM and promote glial-fibrotic transformation by reducing MVD and promoting GMT but does not decrease the cell density in patients with PDR.

show abstract

“…Inhibition of SDF-1 in mice with diabetic nephritis reduces the severity of glomerular sclerosis and prevents proteinuria ( 91 ). Higher levels of SDF-1 have also been found in the vitreous of patients with DR, suggesting its pathogenic effect on eye lesions ( 92 ). SDF-1 promotes a firm adhesion of endothelial cells to the endothelium of the vasculature by increasing the expression of VCAM on endothelial cells; it also promotes the migration and homing of endothelial progenitor cells ( 93 ).…”

Section: Inflammation In Diabetes and Diabetic Retinopathymentioning

confidence: 99%

The role of inflammation in immune system of diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

et al. 2022

View full text Add to dashboard Cite

Diabetic retinopathy is one of the most common complications of diabetes mellitus and the leading cause of low vision and blindness worldwide. Mounting evidence demonstrates that inflammation is a key mechanism driving diabetes-associated retinal disturbance, yet the pathophysiological process and molecular mechanisms of inflammation underlying diabetic retinopathy are not fully understood. Cytokines, chemokines, and adhesion molecules interact with each other to form a complex molecular network that propagates the inflammatory and pathological cascade of diabetic retinopathy. Therefore, it is important to understand and elucidate inflammation-related mechanisms behind diabetic retinopathy progression. Here, we review the current understanding of the pathology and pathogenesis of inflammation in diabetic retinopathy. In addition, we also summarize the relevant clinical trials to further suggest inflammation-targeted therapeutics for prevention and management of diabetic retinopathy.

show abstract

Vitreous levels of vascular endothelial growth factor, stromal cell–derived factor-1α, and angiopoietin-like protein 2 in patients with active proliferative diabetic retinopathy

Cited by 9 publications

References 42 publications

Aqueous humor levels of vascular endothelial growth factor and stromal cell-derived factor-1α in age-related macular degeneration

Aqueous humor levels of vascular endothelial growth factor and stromal cell-derived factor-1α in age-related macular degeneration

Effect of intravitreal ranibizumab on fibrovascular membranes in patients with proliferative diabetic retinopathy

The role of inflammation in immune system of diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

Contact Info

Product

Resources

About