Vitreous preservation of articular cartilage from cryoinjury in rabbits

Abstract

【Abstract】Frozen osteoarticular grafts treated with liquid nitrogen are utilized for joint reconstruction after tumor resection, but the joints may subsequently develop osteoarthritic changes. To preserve articular cartilage from cryoinjury, we modified a vitrification method utilized for embryo cryopreservation and demonstrated in vitro that our vitrification protocol was effective for protecting cartilage from cryoinjury. In this study, we investigated in vivo whether this vitrification method could protect against

Clinical course of grafted cartilage in osteoarticular frozen autografts for reconstruction after resection of malignant bone and soft-tissue tumor involving an epiphysis

“…Preventing cryoinjury is a field of intensive research, especially for cryopreservation of organs. Vitrification using ethylene glycol and sucrose can be used in the freezing procedure to protect cartilage and this has been confirmed in vitro and in vivo [14] , [15] . However, protecting cartilage means protecting tumor cells in the epiphysis and it cannot be denied that vitrification increases the local recurrence rate.…”

Clinical outcomes of frozen autograft reconstruction for the treatment of primary bone sarcoma in adolescents and young adults

“…The advantage of osteoarticular reconstruction is that it has the potential to preserve the joint, especially in young patients; however, high rates of joint degeneration were reported, and these patients eventually require prosthetic joint replacement at skeletal maturity 15 , 31 , 47 . The cartilage is usually thick in children and no degenerative change was observed in skeletally mature AYAs unless the cartilage is affected by a bone tumor; however, the cartilage was reported to be degenerated by freezing methods in previous studies 48 . Our data suggested that the frozen osteoarticular bone in pediatric and AYA patients, which is composed of large amount of chondrocytes, might be fragile especially with regard to articular cartilage of the distal femur or proximal tibia.…”

Vitrification of Intact Porcine Femoral Condyle Allografts Using an Optimized Approach

“… 14 CPA permeation is the essential step to avoid ice formation in the articular cartilage matrix during the cooling/warming processes of vitrification. 12 , 14 , 16 , 17 The development of CPA permeation protocols can be optimized using mathematical modelling. Our group developed an optimized 7-hour stepwise CPA permeation protocol for articular cartilage with 2-mm thickness, 18 and the experimental results confirmed the efficiency of this protocol for 10-mm diameter porcine osteochondral dowels with a promising chondrocyte survival of approximately 75%.…”