Vitrification and levitation of a liquid droplet on liquid nitrogen

Song, Young Seok; Adler, Douglas G.; Xu, Feng; Kayaalp, Emre; Nureddin, Aida; Anchan, Raymond M.; Maas, Richard L.; Demirci, Utkan

doi:10.1073/pnas.0914059107

Cited by 127 publications

(127 citation statements)

References 32 publications

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“…[22][23][24][25][26][27] Quantification of cellular alignment is necessary to validate the effectiveness of these approaches and the engineered microenvironment. Therefore, quantification of cellular alignment and organization with a rapid, accurate, and adaptable method would be significant in assessing the organization of cells in engineered tissues and biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Beyazoglu

Hefner

et al. 2011

Tissue Engineering Part C: Methods

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Cellular alignment plays a critical role in functional, physical, and biological characteristics of many tissue types, such as muscle, tendon, nerve, and cornea. Current efforts toward regeneration of these tissues include replicating the cellular microenvironment by developing biomaterials that facilitate cellular alignment. To assess the functional effectiveness of the engineered microenvironments, one essential criterion is quantification of cellular alignment. Therefore, there is a need for rapid, accurate, and adaptable methodologies to quantify cellular alignment for tissue engineering applications. To address this need, we developed an automated method, binarization-based extraction of alignment score (BEAS), to determine cell orientation distribution in a wide variety of microscopic images. This method combines a sequenced application of median and band-pass filters, locally adaptive thresholding approaches and image processing techniques. Cellular alignment score is obtained by applying a robust scoring algorithm to the orientation distribution. We validated the BEAS method by comparing the results with the existing approaches reported in literature (i.e., manual, radial fast Fourier transform-radial sum, and gradient based approaches). Validation results indicated that the BEAS method resulted in statistically comparable alignment scores with the manual method (coefficient of determination R 2 = 0.92). Therefore, the BEAS method introduced in this study could enable accurate, convenient, and adaptable evaluation of engineered tissue constructs and biomaterials in terms of cellular alignment and organization.

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Section: Introductionmentioning

confidence: 99%

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Beyazoglu

Hefner

et al. 2011

Tissue Engineering Part C: Methods

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“…Vitrification carrier systems generally need manual handling with great manipulation skills, leading to a low throughput process. The approach only fits well with cryopreservation of cells in small volumes such as oocytes 91 but not with MSCs in large volumes. Further, vitrification requires a high concentration of CPAs (6-8 M),…”

Section: Vitrificationmentioning

confidence: 96%

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Yong

Zaman

Xufeng

et al. 2015

Biopreservation and Biobanking

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Mesenchymal stem cells (MSCs) hold many advantages over embryonic stem cells (ESCs) and other somatic cells in clinical applications. MSCs are multipotent cells with strong immunosuppressive properties. They can be harvested from various locations in the human body (e.g., bone marrow and adipose tissues). Cryopreservation represents an efficient method for the preservation and pooling of MSCs, to obtain the cell counts required for clinical applications, such as cell-based therapies and regenerative medicine. Upon cryopreservation, it is important to preserve MSCs functional properties including immunomodulatory properties and multilineage differentiation ability. Further, a biosafety evaluation of cryopreserved MSCs is essential prior to their clinical applications. However, the existing cryopreservation methods for MSCs are associated with notable limitations, leading to a need for new or improved methods to be established for a more efficient application of cryopreserved MSCs in stem cell-based therapies. We review the important parameters for cryopreservation of MSCs and the existing cryopreservation methods for MSCs. Further, we also discuss the challenges to be addressed in order to preserve MSCs effectively for clinical applications.

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“…Some surface chemistry approaches such as Protein G-based strategies are reported to have long-term shelf life exceeding 8 months (42). The shelf life of immobilized antibodies potentially can be prolonged using various biological materials including trehalose, glycerol (43), and silk (44,45) as well as by biopreservation (46) and dry storage approaches (47).…”

Section: Discussionmentioning

confidence: 99%

Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE ² RD) for diagnostics

İnci

Filippini

Baday

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Recent advances in biosensing technologies present great potential for medical diagnostics, thus improving clinical decisions. However, creating a label-free general sensing platform capable of detecting multiple biotargets in various clinical specimens over a wide dynamic range, without lengthy sample-processing steps, remains a considerable challenge. In practice, these barriers prevent broad applications in clinics and at patients' homes. Here, we demonstrate the nanoplasmonic electrical field-enhanced resonating device (NE 2 RD), which addresses all these impediments on a single platform. The NE 2 RD employs an immunodetection assay to capture biotargets, and precisely measures spectral color changes by their wavelength and extinction intensity shifts in nanoparticles without prior sample labeling or preprocessing. We present through multiple examples, a label-free, quantitative, portable, multitarget platform by rapidly detecting various protein biomarkers, drugs, protein allergens, bacteria, eukaryotic cells, and distinct viruses. The linear dynamic range of NE 2 RD is five orders of magnitude broader than ELISA, with a sensitivity down to 400 fg/mL This range and sensitivity are achieved by self-assembling gold nanoparticles to generate hot spots on a 3D-oriented substrate for ultrasensitive measurements. We demonstrate that this precise platform handles multiple clinical samples such as whole blood, serum, and saliva without sample preprocessing under diverse conditions of temperature, pH, and ionic strength. The NE 2 RD's broad dynamic range, detection limit, and portability integrated with a disposable fluidic chip have broad applications, potentially enabling the transition toward precision medicine at the pointof-care or primary care settings and at patients' homes.iosensing platforms have enabled various applications in different fields of clinical medicine such as biomarker/drug discovery and initiation and monitoring of therapy (1-3). However, material cost, accessibility, ease of operation, lack of portability, and complexity in readout remain major challenges for developing robust diagnostic assays (SI Appendix, Table S1). Recent advances in nanotechnology and biosensing have created new avenues to address these issues (4-9). Technically, they have provided integration of high-throughput sampling with readout systems for quantitative detection of disease-specific biotargets. Therefore, they have demonstrated great potential to revolutionize medical diagnostics. However, from a clinical and technological perspective, existing platforms still face several challenges. First, lengthy assay time hinders physicians from making early clinical decisions. Second, examining clinical samples with diverse pH range, ionic content, and ionic strength requires SignificanceBiosensing technologies have significant impact on medical diagnostics but difficulties in the handling of complex biospecimens, portability, and nonlinearity in dynamic detection range present considerable technical bottlenecks in their tran...

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Vitrification and levitation of a liquid droplet on liquid nitrogen

Cited by 127 publications

References 32 publications

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE ² RD) for diagnostics

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Vitrification and levitation of a liquid droplet on liquid nitrogen

Cited by 127 publications

References 32 publications

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE 2 RD) for diagnostics

Contact Info

Product

Resources

About

Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE ² RD) for diagnostics