Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis

Arshad, Usman; Sagheer, Masroor; González-Silvestry, Fátima B.; Hassan, Mubbashar; Sosa, Froylan

doi:10.1016/j.cryobiol.2021.06.007

Cited by 20 publications

(10 citation statements)

References 67 publications

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“…HSPs play an essential role in cell protection and heat tolerance during heat stress, providing protein folding and refolding, promoting protein aggregation, and overcoming potentially harmful interactions [ 58 ]. A study conducted by [ 59 ], evaluating the vitrification method for in vitro and in vivo survival of bos taurus embryos, demonstrated that this technique can promote the transcription of the HSPA5 gene in response to cellular stress. Although the focus of our work is not the same, we also found the HSPA5 gene, demonstrating that, in some way, the stress response can affect the metabolism of enriched pathways associated with this gene in our study.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle

Reis,

Carvalho,

Espigolan

et al. 2023

Metabolites

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The meat market has enormous importance for the world economy, and the quality of the product offered to the consumer is fundamental for the success of the sector. In this study, we analyzed a database which contained information on 2470 animals from a commercial farm in the state of São Paulo, Brazil. Of this total, 2181 animals were genotyped, using 777,962 single-nucleotide polymorphisms (SNPs). After quality control analysis, 468,321 SNPs provided information on the number of genotyped animals. Genome-wide association analyses (GWAS) were performed for the characteristics of the rib eye area (REA), subcutaneous fat thickness (SFT), shear force at 7 days’ ageing (SF7), and intramuscular fat (IMF), with the aid of the single-step genomic best linear unbiased prediction (ssGBLUP) method, with the purpose of identifying possible genomic windows (~1 Mb) responsible for explaining at least 0.5% of the genetic variance of the traits under analysis (≥0.5%). These genomic regions were used in a gene search and enrichment analyses using MeSH terms. The distributed heritability coefficients were 0.14, 0.20, 0.18, and 0.21 for REA, SFT, SF7, and IMF, respectively. The GWAS results indicated significant genomic windows for the traits of interest in a total of 17 chromosomes. Enrichment analyses showed the following significant terms (FDR ≤ 0.05) associated with the characteristics under study: for the REA, heat stress disorders and life cycle stages; for SFT, insulin and nonesterified fatty acids; for SF7, apoptosis and heat shock proteins (HSP27); and for IMF, metalloproteinase 2. In addition, KEGG (Kyoto encyclopedia of genes and genomes) enrichment analysis allowed us to highlight important metabolic pathways related to the studied phenotypes, such as the growth hormone synthesis, insulin-signaling, fatty acid metabolism, and ABC transporter pathways. The results obtained provide a better understanding of the molecular processes involved in the expression of the studied characteristics and may contribute to the design of selection strategies and future studies aimed at improving the productivity of Nellore cattle.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle

Reis,

Carvalho,

Espigolan

et al. 2023

Metabolites

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“…Vitrification of ovarian tissue is becoming an alternative to slow freezing [6,7]. Vitrification can achieve a high success rate [8]; enable the establishment and development of an oocyte bank [9,10]; and provide opportunities for infertile patients, young female cancer patients and other people who want to retain fertility [11]. However, the vitrification process exerts strong physical stress, with severe osmotic pressure and temperature changes.…”

Section: Introductionmentioning

confidence: 99%

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Chen

Zhang

et al. 2022

Clin Epigenet

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Cryopreservation has important application in assisted reproductive technology (ART). The vitrification technique has been widely used in the cryopreservation of oocytes and embryos, as a large number of clinical results and experimental studies have shown that vitrification can achieve a higher cell survival rate and preimplantation development rate and better pregnancy outcomes. Ovarian tissue vitrification is an alternative method to slow freezing that causes comparatively less damage to the original follicular DNA. At present, sperm preservation mainly adopts slow freezing or rapid freezing (LN2 vapor method), although the vitrification method can achieve higher sperm motility after warming. However, due to the use of high-concentration cryoprotectants and ultra-rapid cooling, vitrification may cause strong stress to gametes, embryos and tissue cells, resulting in potentially adverse effects. Imprinted genes are regulated by epigenetic modifications, including DNA methylation, and show single allele expression. Their accurate regulation and correct expression are very important for the placenta, fetal development and offspring health. Considering that genome imprinting is very sensitive to changes in the external environment, we comprehensively summarized the effect of cryopreservation—especially the vitrification method in ART—on imprinted genes. Animal studies have found that the vitrification of oocytes and embryos can have a significant impact on some imprinted genes and DNA methylation, but the few studies in humans have reported almost no influence, which need to be further explored. This review provides useful information for the safety assessment and further optimization of the current cryopreservation techniques in ART.

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“…However, in vitro experiments often do not accurately represent embryonic survival in vivo , and, while in vitro quality parameters are sometimes superior in vitrified/warmed (V/W) over frozen/thawed (F/T) embryos, such differences are not observed in pregnancy rates ( 4 , 26 ). Indeed, ET reports with large numbers of V/W and F/T IVP embryos indicate that both cryopreservation systems perform equally in pregnancies reaching term, sometimes with rates close to fresh IVP embryos ( 4 , 13 , 19 , 27 – 30 ).…”

Section: Introductionmentioning

confidence: 99%

Fitness of calves born from in vitro-produced fresh and cryopreserved embryos

et al. 2022

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In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T), in vitro-produced (IVP) embryos, differ in their physiology and survival from fresh embryos. In this study, we analyzed the effects of embryo cryopreservation techniques on the offspring. IVP embryos cultured with albumin and with or without 0.1% serum until Day 6, and thereafter in single culture without protein, were transferred to recipients on Day 7 as F/T, V/W, or fresh, resulting in N = 24, 14, and 13 calves, respectively. Calves were clinically examined at birth, and blood was analyzed before and after colostrum intake (Day 0), and subsequently on Day 15 and Day 30. On Day 0, calves from V/W and F/T embryos showed increased creatinine and capillary refill time (CRT) and reduced heartbeats. Calves from F/T embryos showed lower PCO2, hemoglobin, and packed cell volume than calves from V/W embryos while V/W embryos led to calves with increased Na+ levels. Colostrum effects did not differ between calves from fresh and cryopreserved embryos, indicating similar adaptive ability among calves. However, PCO2 did not decrease in calves from V/W embryos after colostrum intake. Serum in culture led to calves with affected (P < 0.05) temperature, CRT, HCO3-, base excess (BE), TCO2, creatinine, urea, and anion gap. On Day 15, the effects of embryo cryopreservation disappeared among calves. In contrast, Day 30 values were influenced by diarrhea appearance, mainly in calves from V/W embryos (i.e., lower values of TCO2, HCO3-, and BE; and increased glucose, anion gap, and lactate), although with no more clinical compromise than calves from fresh and F/T embryos. Diarrhea affected PCO2 and Na+ in all groups. Embryo cryopreservation, and/or culture, yield metabolically different calves, including effects on protein and acid–base metabolism.

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Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis

Cited by 20 publications

References 67 publications

Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle

Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Fitness of calves born from in vitro-produced fresh and cryopreserved embryos

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