Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured and fertilized in vitro

Kuwayama, M.; Hamano, Seizo; Nagai, T.

doi:10.1530/jrf.0.0960187

Cited by 82 publications

(30 citation statements)

References 10 publications

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“…Fresh-vitro embryos used in the present study were obtained from a commercial supplier and their production details are described elsewhere [11]. Ovaries were obtained from a local abattoir and transported to the laboratory.…”

Section: Methodsmentioning

confidence: 99%

Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos.

Kojima¹,

Akagi²,

Zeniya³

et al. 1996

Journal of Reproduction and Development

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Abstract. The aims of the present study were twofold. The first aim was to examine whether or not early pregnancy-associated thrombocytopenia (EPAT) occurred in cows following nonsurgical embryo transfer by observing the daily patterns of the concentration of peripheral platelets (PLT) during the preimplantation period of pregnancy. The other goal was to assess the use of PLT count as a diagnosis of early pregnancy in cows. In Experiment 1, out of 50 Holstein parous cows, 40 had one embryo nonsurgically transferred to each uterine horn on Day 6 or 7 (Day 0=onset of standing estrus). The remaining 10 cows served as controls having no embryos transferred. Blood was taken daily at the same time, from Day 6 or 7 following estrus (the day when the embryos were transferred in the non-controls) to the day of return to estrus for control cows and non-pregnant cows, or to Day 36 for pregnant cows. Bilateral embryo transfers resulted in 9 twin, 14 single, and 17 nonpregnancies. Some recipient cows showed an increase in PLT following embryo transfer, while others displayed a decrease in PLT. If significant PLT change was characterized either by a decrease or an increase of >20% compared to the value of pre-transfer, the proportions of cows indicating significant change were 78% (7/9) in twin, 64% (9/14) in single, 76% (13/17) in nonpregnancies and 30% (3/10) in controls. The proportions in twin (P<0.05), single (P<0.05) and nonpregnancies (P<0.01) were higher than that in controls. The effect of the particular group on PLT was significant (P<0.0025). In Experiment 2, each of several in vitro-produced blastocysts were nosurgically transferred to 9 Japanese Black cows on Day 7 or 8 following onset of estrus. Seven days after embryo transfer, an attempt to recover the previously transferred embryos by nonsurgical flushing was performed. Embryos could be recovered on Day 14 or 15 from 5 animals, whose blood was taken from the day of embryo transfer to the next day of embryo recovery and during the corresponding days of the following estrous cycle. For PLT, differences were detected between the both phases of 3 out of the 5 cows. The individual PLT variation in response means that EPAT does not necessarily occur in cows following embryo transfer, and that the use of PLT counts is unlikely to be clinically useful in assessing early pregnancy in cows. These results may suggest, however, that maternal platelet activation would occur during early pregnancy in cattle.

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Section: Methodsmentioning

confidence: 99%

Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos.

Kojima¹,

Akagi²,

Zeniya³

et al. 1996

Journal of Reproduction and Development

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“…In attempts to avoid the critical minimum cell volume, common methods for oocyte and zygote vitrification have incorporated at least three-step equilibration processes whereby cells are manually pipetted into subsequently higher levels of permeating CPA concentrations (Kuwayama et al, 2005a(Kuwayama et al, , 2005bPegg, 2010;Smith et al, 2010) allowing the cell to shrink in a specific interval. It has been experimentally demonstrated that the use of a higher number of 'steps' leads to enhanced cryosurvival and developmental outcomes (Kuwayama et al, 1992;Otoi et al, 1998); however, such protocols have not been adopted due to impracticality. The use of an automated, continuously and gradually changing CPA concentration exposure is a logical extension of this previous work, as a continuously gradual change in CPA concentration is fundamentally an 'unlimited amount of steps'.…”

Section: Cryopreservation With Microfluidicsmentioning

confidence: 99%

Application of microfluidic technologies to human assisted reproduction

Smith

Takayama

2017

Mol. Hum. Reprod.

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Microfluidics can be considered both a science and a technology. It is defined as the study of fluid behavior at a sub-microliter level and the investigation into its application to cell biology, chemistry, genetics, molecular biology and medicine. There are at least two characteristics of microfluidics, mechanical and biochemical, which can be influential in the field of mammalian gamete and preimplantation embryo biology. These microfluidic characteristics can assist in basic biological studies on sperm, oocyte and preimplantation embryo structure, function and environment. The mechanical and biochemical characteristics of microfluidics may also have practical and/or technical application(s) to assisted reproductive technologies (ART) in rodents, domestic species, endangered species and humans. This review will consider data in mammals, and when available humans, addressing the potential application(s) of microfluidics to assisted reproduction. There are numerous sequential steps in the clinical assisted reproductive laboratory process that work, yet could be improved. Cause and effect relations of procedural inefficiencies can be difficult to identify and/or remedy. Data will be presented that consider microfluidic applications to sperm isolation, oocyte cumulus complex isolation, oocyte denuding, oocyte mechanical manipulation, conventional insemination, intracytoplasmic sperm injection, embryo culture, embryo analysis and oocyte and embryo cryopreservation. While these studies have progressed in animal models, data with human gametes and embryos are significantly lacking. These data from clinical trials are requisite for making future evidence-based decisions regarding the application of microfluidics in human ART.

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“…The procedures for in vitro maturation, fertilization and culture of transferred embryos have been described in detail elsewhere 12 . Pregnancy and fetal number were diagnosed around days 30, 45, 60 and 90 by transrectal ultrasonography and confirmed at parturition.…”

Section: Animalsmentioning

confidence: 99%

Changes in Plasma Estrogen Concentrations during the First Trimester of Gestation in Dairy Cows: Comparison with the Origin of Embryos and Fetal Number

Hirako

Takahashi

et al. 2003

JARQ

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The objectives of this study were to determine the profiles of plasma estrogen concentrations during the first trimester of gestation and to correlate them with the origin of the embryos and fetal number. Pregnancies were induced either by artificial insemination (AI) on the day of estrus (day 0) or by transfer of 1 or 2 blastocysts produced in vitro (IVF-ET) on day 7. Five singleton-bearing cows impregnated by AI, and 6 singleton and 6 twin pregnancies induced by IVF-ET were used in the experiment. Blood was collected by jugular venipuncture at 2 to 5-day intervals until day 100. The concentrations of plasma estrone, estradiol-17β and estrone sulfate were measured by using a radioimmunoassay. Concentrations of all the estrogens fluctuated at low levels until day 50 in all the groups. Thereafter, the concentration of estrone sulfate gradually increased, whereas those of estrone and estradiol-17β remained at basal levels until day 80. The concentration of estrone sulfate after day 50 was affected by the day of gestation and the number of fetuses. After day 80, the concentration of estrone sulfate increased drastically, coinciding with the increases in the estrone and estradiol-17β concentrations. The rate of increase in the concentration of estrone sulfate during days 80 to 100 was the highest among all the estrogens. In the singleton pregnancies, the concentrations of estrone and estrone sulfate between days 80 and 100 were higher in the artificially inseminated cows than in the IVF-ET cows. In the pregnancies induced by IVF-ET, estrone sulfate levels were higher in the twin-bearing cows than in the singleton-bearing cows during days 50 to 80 and 80 to 100. These results suggest that the concentrations of estrogens in bovine peripheral blood plasma could be applied for monitoring fetoplacental development in the first trimester of gestation.Discipline: Animal industry Additional key words: estradiol-17β, estrone, estrone sulfate, in vitro fertilization and multiple pregnancies JARQ 37 (3), 195 -200 (2003) http://www.jircas.affrc.go.jp Present address:

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Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured and fertilized in vitro

Cited by 82 publications

References 10 publications

Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos.

Evidence of Platelet Activation Associated with Establishment of Pregnancy in Cows with Transferred Embryos.

Application of microfluidic technologies to human assisted reproduction

Changes in Plasma Estrogen Concentrations during the First Trimester of Gestation in Dairy Cows: Comparison with the Origin of Embryos and Fetal Number

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