Vitrification of human ovarian tissue: a practical and relevant alternative to slow freezing

Sanfilippo, Sandra; Canis, Michel; Smitz, Johan; Sion, Benoı̂t; Darcha, Claude; Janny, L.; Brugnon, Florence

doi:10.1186/s12958-015-0065-5

Cited by 54 publications

(17 citation statements)

References 30 publications

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“…Morphological comparison was chosen because it has been proven effective for assessing viability in several studies. 25,26 In our study, 46% of the follicles analyzed were present in the control group, which showed a great loss of follicles after thawing. Variations may probably occur because of the size of the fragments, type and duration of exposure to cryoprotectants, and the heterogeneity of ovarian tissues, when compared with the amount of follicles and their stages.…”

Section: Discussionmentioning

confidence: 50%

Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model

Campos

Guedes

Rodrigues³

et al. 2016

Rev Bras Ginecol Obstet

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Objective To assess the viability of bovine ovarian tissue after cryopreservation through either slow freezing or vitrification, and to compare it to that of control tissue by performing morphological analyses. Methods The study included 20 bovine ovarian cortex fragments that were divided into control, vitrification, and slow freezing groups. Each group consisted of four fragments of the same ovary, two fixed without cultivation, and two fixed with cultivation. Tissues were evaluated based on follicular morphology immediately after heating and after 7 days of culture, and compared with the control group. Results A total of 240 fragments were analyzed, generating a sample of 1,344 follicles without cultivation and 552 with cultivation. When the non-cultivated samples were classified as non-atretic follicles, 572 were found in the control group, 289 in the vitrification group, and 373 in the slow freezing group, showing no significant differences. When classified as atretic, 46 follicles were found in the control group, 23 in the vitrification group, and 41 in the slow freezing group, also showing no statistical difference. In the post-culture sample, an evolution of the follicular stages could be observed. This finding was important to support that the follicles considered non-atretic in the non-cultivated group were actually viable in the morphological evaluation. Conclusion With no differences between the protocols, vitrification was shown to be an advanced and alternative method for patients who will undergo treatments that

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Section: Discussionmentioning

confidence: 50%

Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model

Campos

Guedes

Rodrigues³

et al. 2016

Rev Bras Ginecol Obstet

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show abstract

“…Although there is a general agreement on the negative effects of both slow freezing and vitrification on the quality of follicles and SCs in thawed tissues (9,(38)(39)(40)(41), some slow-freezing and vitrification procedures were reported to not damage the follicle quality (9,16). Herein, follicle quality in fresh and vitrified samples was evaluated classifying follicles into three grades on the basis of histologic analysis (12).…”

Section: Discussionmentioning

confidence: 99%

Successful slush nitrogen vitrification of human ovarian tissue

Talevi

Barbato

Fiorentino

et al. 2016

Fertility and Sterility

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“…The first live birth from frozen to thawed ovarian cortex after orthotopic autotransplantation was reported by a Belgian group [18]. To date, slow freezing has resulted in 36 documented live births worldwide after orthotopic autotransplantation [42][43][44].…”

Section: Methods Of Gonadal Tissue Cryopreservation Slow Freezingmentioning

confidence: 99%

Fertility preservation through gonadal cryopreservation

Devi

Goel

2016

Reprod Medicine & Biology

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Fertility preservation is an area of immense interest in today's society. The most effective and established means of fertility preservation is cryopreservation of gametes (sperm and oocytes) and embryos. Gonadal cryopreservation is yet another means for fertility preservation, especially if the gonadal function is threatened by premature menopause, gonadotoxic cancer treatment, surgical castration, or diseases. It can also aid in the preservation of germplasm of animals that die before attaining sexual maturity. This is especially of significance for valuable, rare, and endangered animals whose population is affected by high neonatal/juvenile mortality because of diseases, poor management practices, or inbreeding depression. Establishing genome resource banks to conserve the genetic status of wild animals will provide a critical interface between ex-situ and in-situ conservation strategies. Cryopreservation of gonads effectively lengthens the genetic lifespan of individuals in a breeding program even after their death and contributes towards germplasm conservation of prized animals. Although the studies on domestic animals are quite promising, there are limitations for developing cryopreservation strategies in wild animals. In this review, we discuss different options for gonadal tissue cryopreservation with respect to humans and to laboratory, domestic, and wild animals. This review also covers recent developments in gonadal tissue cryopreservation and transplantation, providing a systematic view and the advances in the field with the possibility for its application in fertility preservation and for the conservation of germplasm in domestic and wild species.

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Vitrification of human ovarian tissue: a practical and relevant alternative to slow freezing

Cited by 54 publications

References 30 publications

Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model

Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model

Successful slush nitrogen vitrification of human ovarian tissue

Fertility preservation through gonadal cryopreservation

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