Vitrification of Plant Cell Suspensions

Reinhoud, P. J.; Uragami, Atsuko; Sakai, Akira; Iren, F. van

doi:10.1385/0-89603-296-5:113

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…For example, cryopreserved somatic embryos of coriander were first recovered on a Gelrite-solidified medium, but agar was used for embryo maturation and plant development [ 127 ]. Several studies suggested that undifferentiated cell cultures generally yield better results when recovered on agarose [ 131 ]. Van Eck and Keen [ 69 ] compared medium solidified with 0.75% agarose or 0.8% agar for the post-cryogenic recovery of tobacco cell culture and found that cells recovered much faster on agarose-solidified medium and had approximately four times the cell mass compared to agar medium.…”

Section: Strategy 2 Chemical Environment: Modifications Of Recovery M...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

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Cryopreservation is an effective option for the long-term conservation of plant genetic resources, including vegetatively propagated crops and ornamental plants, elite tree genotypes, threatened plant species with non-orthodox seeds or limited seed availability, as well as cell and root cultures useful for biotechnology. With increasing success, an arsenal of cryopreservation methods has been developed and applied to many species and material types. However, severe damage to plant material accumulating during the multi-step cryopreservation procedure often causes reduced survival and low regrowth, even when the optimized protocol is applied. The conditions at the recovery stage play a vital role in supporting material regrowth after cryopreservation and, when optimized, may shift the life-and-death balance toward a positive outcome. In this contribution, we provide an overview of the five main strategies available at the recovery stage to improve post-cryopreservation survival of in vitro plant materials and their further proliferation and development. In particular, we discuss the modification of the recovery medium composition (iron- and ammonium-free), exogenous additives to cope with oxidative stress and absorb toxic chemicals, and the modulation of medium osmotic potential. Special attention is paid to plant growth regulators used at various steps of the recovery process to induce the desired morphological response in cryopreserved tissues. Given studies on electron transport and energy provision in rewarmed materials, we discuss the effects of light-and-dark conditions and light quality. We hope that this summary provides a helpful guideline and a set of references for choosing the recovery conditions for plant species that have not been cryopreserved. We also propose that step-wise recovery may be most effective for materials sensitive to cryopreservation-induced osmotic and chemical stresses.

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Section: Strategy 2 Chemical Environment: Modifications Of Recovery M...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

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“…The vitrification state is achieved by the dehydration of cells, either by the treatment of plant tissue in a mixture of highly concentrated cryoprotective solutions (penetrating and non-penetrating agents) or by physical desiccation and subsequent very rapid cooling by direct immersion in LN. This procedure is simple, easy to apply, and does not need specific apparatus for controlled cooling; it has high reproducibility, and it has been applied successfully to a wide range of species using several explant types [16,18]. Therefore, it is amenable and more widely used compared to slow-controlled systems [14].…”

Section: History Principles and Fundamentals Of Cryopreservationmentioning

confidence: 99%

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Merzougui

Benelli̇

Boullani

et al. 2023

Plants

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Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (−196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.

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“…To reduce the maintenance cost and mitigate the risks of (epi)genetic variations and human errors associated with long-term in vitro maintenance, cryogenic (−196 °C) storage has been successfully applied to some plant in vitro collections of high agricultural and horticultural importance [ 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. Likewise, cold storage (−80–+4 °C) and cryopreservation have been implemented to conserve the diversity of biotechnological materials, including plant cell cultures, adventitious and hairy root cultures, and algae, although large-scale low-temperature conservation is still limited to few examples [ 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…Biology 2023, 12, x FOR PEER REVIEW 3 of 45 plant cell cultures, adventitious and hairy root cultures, and algae, although large-scale low-temperature conservation is still limited to few examples [28][29][30][31]. The K.A.…”

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confidence: 99%

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

Yuorieva,

Sinetova,

Messineva

et al. 2023

Biology

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Ex situ collections of algae, cyanobacteria, and plant materials (cell cultures, hairy and adventitious root cultures, shoots, etc.) maintained in vitro or in liquid nitrogen (−196 °C, LN) are valuable sources of strains with unique ecological and biotechnological traits. Such collections play a vital role in bioresource conservation, science, and industry development but are rarely covered in publications. Here, we provide an overview of five genetic collections maintained at the Institute of Plant Physiology of the Russian Academy of Sciences (IPPRAS) since the 1950–1970s using in vitro and cryopreservation approaches. These collections represent different levels of plant organization, from individual cells (cell culture collection) to organs (hairy and adventitious root cultures, shoot apices) to in vitro plants. The total collection holdings comprise more than 430 strains of algae and cyanobacteria, over 200 potato clones, 117 cell cultures, and 50 strains of hairy and adventitious root cultures of medicinal and model plant species. The IPPRAS plant cryobank preserves in LN over 1000 specimens of in vitro cultures and seeds of wild and cultivated plants belonging to 457 species and 74 families. Several algae and plant cell culture strains have been adapted for cultivation in bioreactors from laboratory (5–20-L) to pilot (75-L) to semi-industrial (150–630-L) scale for the production of biomass with high nutritive or pharmacological value. Some of the strains with proven biological activities are currently used to produce cosmetics and food supplements. Here, we provide an overview of the current collections’ composition and major activities, their use in research, biotechnology, and commercial application. We also highlight the most interesting studies performed with collection strains and discuss strategies for the collections’ future development and exploitation in view of current trends in biotechnology and genetic resources conservation.

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Vitrification of Plant Cell Suspensions

Cited by 9 publications

References 4 publications

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

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