Vocal and physical phenotypes of calsyntenin2 knockout mouse pups model early-life symptoms of the autism spectrum disorder

Klenova, Anna V.; Volodin, Ilya A.; Volodina, Elena V.; Ranneva, S.V.; Amstislavskaya, Tamara G.; Lipina, Tatiana V.

doi:10.1016/j.bbr.2021.113430

Cited by 10 publications

(11 citation statements)

References 101 publications

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“…However, the AFO group consistently showed lower entropy scores than the YFO group across all postnatal stages (fathers’ age, F (1, 390.1) = 60.200, p < 0.001; postnatal day, F (3, 384.0) = 2.748, p = 0.043; body weight, F (1, 372.4) = 12.899, p < 0.001; fathers’ age × postnatal day, F (3, 393.8) = 1.841, p = 0.139, mixed model). For reflecting the complexity of syllable usage from a different angle, we calculated the one-note ratio according to a previous study ( Klenova et al., 2021 ). A higher one-note ratio indicated the less complex syllable usage.…”

Section: Resultsmentioning

confidence: 99%

“…A limitation here is that no standard criteria can be used to determine the typical and atypical individuals in neonatal USVs. A previous study showed that the one-note ratio could reflect the complexity of syllable usage, which may provide a potential to identify the atypical USV pattern ( Klenova et al., 2021 ). As we expected, the atypical pups indeed demonstrated a higher one-note ratio (i.e., less complex syllable usage) ( Figure S7 ).…”

Section: Discussionmentioning

confidence: 99%

“…The lower body weight gain was consistently shown in the atypical pups. In other ASD genetic models such as Clstn2 -KO pups, abnormal physical growth (i.e., lower body weight and the increased head-to-body ratio) was also reported ( Klenova et al., 2021 ). Therefore, our model exhibited a similar physical alteration to other mouse models of advanced paternal age and ASD.…”

Section: Discussionmentioning

confidence: 99%

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Advanced paternal age diversifies individual trajectories of vocalization patterns in neonatal mice

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Advanced paternal age diversifies individual trajectories of vocalization patterns in neonatal mice

et al. 2022

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“…In addition, a change in synapse architectonics was found in Clstn2-KO mice in the medial prefrontal cortex and hippocampus (Ranneva et al, 2020). Moreover, calsintenin-2 knockout (Clstn2-KO) mice demonstrate signs characteristic for ASD, such as hyperactivity, stereotypy, insufficient spatial learning and memory, altered social behavior with impaired ultrasonic vo calization (Lipina et al, 2016;Ranneva et al, 2017;Klenova et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Neuronal density in the brain cortex and hippocampus in Clsnt2-KO mouse strain modeling autistic spectrum disorder

et al. 2022

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Autistic spectrum disorders (ASD) represent conditions starting in childhood, which are characterized by difficulties with social interaction and communication, as well as non-typical and stereotyping models of behavior. The mechanisms and the origin of these disorders are not yet understood and thus far there is a lack of prophylactic measures for these disorders. The current study aims to estimate neuronal density in the prefrontal cortex and four hippocampal subfields, i. e. СA1, СA2, СA3, and DG in Clstn2-KO mice as a genetic model of ASD. In addition, the level of neurogenesis was measured in the DG area of the hippocampus. This mouse strain was obtained by a knockout of the calsinthenin-2 gene (Clsnt2) in C57BL/6J mice; the latter (wild type) was used as controls. To estimate neuronal density, serial sections were prepared on a cryotome for the above-mentioned brain structures with the subsequent immunohistochemical labeling and confocal microscopy; the neuronal marker (anti-NeuN) was used as the primary antibody. In addition, neurogenesis was estimated in the DG region of the hippocampus; for this purpose, a primary antibody against doublecortin (anti-DCX) was used. In all cases Goat anti-rabbit IgG was used as the secondary antibody. The density of neurons in the CA1 region of the hippocampus was lower in Clstn2-KO mice of both sexes as compared with controls. Moreover, in males of both strains, neuronal density in this region was lower as compared to females. Besides, the differences between males and females were revealed in two other hippocampal regions. In the CA2 region, a lower density of neurons was observed in males of both strains, and in the CA3 region, a lower density of neurons was also observed in males as compared to females but only in C57BL/6J mice. No difference between the studied groups was revealed in neurogenesis, nor was it in neuronal density in the prefrontal cortex or DG hippocampal region. Our new findings indicate that calsyntenin-2 regulates neuronal hippocampal density in subfield-specific manner, suggesting that the CA1 neuronal subpopulation may represent a cellular target for earlylife preventive therapy of ASD.

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“…In non-human mammals NLP occur, for example, in the ultrasonic vocalizations of rodents [e.g. 57 – 60 ], but have also been reported in the whistle vocalizations of camels [ 61 ], the bugles of North American wapitis Cervus canadensis [ 62 ], in African wild dogs Lycaon pictus [ 63 ], dog-wolf mixes [ 64 ], domestic dogs Canis lupus forma domestica , piglets Sus scrofa forma domestica , Japanese macaques Macaca fuscata [ 65 ], rhesus macaques Macaca mulatta [ 51 ], common chimpanzees Pan troglodytes [ 66 ] and West Indian manatees Trichechus manatus [ 67 ].…”

Section: Introductionmentioning

confidence: 99%

Acoustic structure and information content of trumpets in female Asian elephants (Elephas maximus)

et al. 2021

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Most studies on elephant vocal communication have focused on the low-frequency rumble, with less effort on other vocalization types such as the most characteristic elephant call, the trumpet. Yet, a better and more complete understanding of the elephant vocal system requires investigating other vocalization types and their functioning in more detail as well. We recorded adult female Asian elephants (Elephas maximus) at a private facility in Nepal and analyzed 206 trumpets from six individuals regarding their frequency, temporal and contour shape, and related acoustic parameters of the fundamental frequency. We also tested for information content regarding individuality and context. Finally, we recorded the occurrence of non-linear phenomena such as bifurcation, biphonation, subharmonics and deterministic chaos. We documented a mean fundamental frequency ± SD of 474 ± 70 Hz and a mean duration ± SD of 1.38 ± 1.46 s (Nindiv. = 6, Ncalls = 206). Our study reveals that the contour of the fundamental frequency of trumpets encodes information about individuality, but we found no evidence for trumpet subtypes in greeting versus disturbance contexts. Non-linear phenomena prevailed and varied in abundance among individuals, suggesting that irregularities in trumpets might enhance the potential for individual recognition. We propose that trumpets in adult female Asian elephants serve to convey an individual’s identity as well as to signal arousal and excitement to conspecifics.

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Vocal and physical phenotypes of calsyntenin2 knockout mouse pups model early-life symptoms of the autism spectrum disorder

Cited by 10 publications

References 101 publications

Advanced paternal age diversifies individual trajectories of vocalization patterns in neonatal mice

Advanced paternal age diversifies individual trajectories of vocalization patterns in neonatal mice

Neuronal density in the brain cortex and hippocampus in Clsnt2-KO mouse strain modeling autistic spectrum disorder

Acoustic structure and information content of trumpets in female Asian elephants (Elephas maximus)

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