Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study

Graupp, Matthias; Kiesler, Karl; Friedrich, Gerhard; Ainödhofer, Herwig; Gruber, Hans-Jürgen; Kieslinger, Petra; Saxena, Amulya K.; Hirano, Shigeru; Gugatschka, Markus

doi:10.1016/j.jvoice.2013.11.005

Cited by 18 publications

(18 citation statements)

References 27 publications

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“…Our present findings imply that HGF is not a mitogen of VFFs. This idea does not contradict the finding of previous works, as none of them directly represented the mitogenic effect of HGF …”

Section: Discussionsupporting

confidence: 59%

“…The VFFs used in the present study, from 19‐month‐old rats, would thus not be considered elderly VFFs according to this standard. However, several reports described physiological differences even between 3‐month‐old and 11‐month‐old rat VFFs, and that finding is meaningful as it indicates that age‐related changes in mammals start even from middle age.…”

Section: Discussionmentioning

confidence: 88%

“…Vocal fold fibroblasts (VFFs) are thought to play major roles in age‐related changes, because extracellular matrix (ECM) is primarily produced by the VFFs. Fibroblasts are widely distributed in many organs, but their characteristics and manner of aging could change depending on their origin or biological conditions . Few studies directly compared aged VFFs with young VFFs in their naïve condition, and only the following characteristics were implied in aged VFFs: impaired proliferative capacity, reduced hyaluronan production, and maintained or reduced collagen production.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of aged rat vocal fold fibroblasts

et al. 2018

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Objectives/Hypothesis To elucidate the aging physiology of the vocal folds, we examined the characters of aged vocal fold fibroblasts (VFFs) in various conditions. Study Design In vitro study. Methods VFFs from young (12‐week‐old) and aged (19‐month‐old) Sprague‐Dawley rats were compared. Proliferative capacity, ratio of myofibroblast to fibroblast, myofibroblast function, and extracellular matrix production were examined in the following conditions: naïve, basic fibroblast growth factor (bFGF) supplemented, and hepatocyte growth factor (HGF) supplemented. Results Aged VFFs demonstrated reduced proliferation by cell counting, though the ratio of Ki‐67–positive cells showed no difference. Aged VFFs exhibited an increased expression of α‐smooth muscle actin (α‐SMA); however, they demonstrated no enhanced contractile ability in a gel contraction assay. Type I collagen protein was increased age dependently, accompanied with decreased Mmp1 and unchanged Col1a1 transcription. Type I collagen protein and α‐SMA represented quite similar reduction patterns to bFGF or HGF administration. Conclusions The following possible characteristics of aged VFFs were implied: long duration of mitosis, increased myofibroblast population size with certain dysfunctions, reduced type I collagen turnover, and correlation between α‐SMA expression and type I collagen metabolism. Further investigations of these features will help to clarify presbyphonia's pathology and establish treatment strategies. Level of Evidence NA Laryngoscope, 129:E94–E101, 2019

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Characterization of aged rat vocal fold fibroblasts

et al. 2018

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“…Previously, bFGF and hepatocyte growth factor (HGF) were shown to increase vocal fold fibroblast hyalurionic acid production and decrease collagen production in vitro and in vivo in animal models. [36][37][38][39][40] Only bFGF has been used in vivo in humans via multiple vocal fold injections or implantation of a gelatin sponge impregnated with bFGF. Patients with vocal fold scar, sulcus, or presbylarynges appeared to benefit from this treatment, though the sample size was small.…”

Section: Discussionmentioning

confidence: 99%

Small Intestinal Submucosa Implantation for the Possible Treatment of Vocal Fold Scar, Sulcus, and Superficial Lamina Propria Atrophy

Pitman

Cabin

Iacob

2015

Ann Otol Rhinol Laryngol

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In the canine model, SIS appears safe for SLP grafting. Inflammation is similar to that of sham surgery. Small intestinal submucosa results in newly generated HA without concomitant fibrosis. Small intestinal submucosa has potential to be used in treatment of disorders with SLP, including vocal fold scar, sulcus, and atrophy. Studies evaluating the effect of SIS implantation on vocal fold function, as well as the ultimate fate of the graft, are required.

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“…The MFe mucosa has a distinctive three‐dimensional (3D) structure consisting of dense masses of macula flava stellate cells (MF‐SCs) and various ECM components including collagen, reticular fiber, elastin, and hyaluronic acid. The VF fibroblasts, located in the LP, maintain VF microenvironments by producing ECM components such as hyaluronic acid . Although the roles of MF‐SCs in the VF mucosa have not been fully described, there are reports of their roles in cell growth, VF development, and ECM metabolic activity, which are significant roles affecting the physiologic characteristics of the LP .…”

Section: Introductionmentioning

confidence: 99%