Background: Theater actors are a subgroup of professional voice users who may present their performances at a high-pressure vocal demand, like loud talking, shouting, or even stage singing. Disturbances in auditory-perceptual or acoustic parameters of voice are commonly experienced by theater actors and are known as a frequent occupational hazard for them. Objectives: This study aimed to examine the relationship between auditory-perceptual and acoustic parameters of the voice in Iranian theater actors. Methods: This descriptive-analytical study was conducted on 45 Iranian theater actors, including 22 females and 23 males, with a mean age of 30.76 ± 7 years. The auditory-perceptual evaluation was performed using the Persian version of the Consensus Auditory-perceptual Evaluation of Voice (CAPE-V). Acoustic characteristics, including mean fundamental frequency (F0), local shimmer, local jitter, and harmonic-to-noise ratio (HNR), were extracted from the subjects’ “speech sample.” Correlation analyses were performed to examine the relationship between the measures of auditory-perceptual and acoustic voice characteristics. Results: The overall auditory-perceptual severity was negatively correlated with mean F0 (r = -0.592, P < 0.05) and HNR (r = - 0.324, P < 0.05). Roughness was also negatively correlated with voice mean F0 (r = -0.629, P < 0.05) and HNR (r = -0.322, P < 0.05) and positively correlated with voice local jitter (r = 0.310, P < 0.05). We also found that pitch was negatively correlated with voice mean F0 (r = -0.309, P < 0.05) and positively correlated with voice local jitter (r = 0.292, P = 0.050). Conclusions: Acoustic measures, including F0, HNR, and jitter, correlate with auditory perceptual parameters of the voice quality in Iranian theater actors. These findings highlight the significance of acoustic parameters in assessing voice quality in professional voice users such as theater actors.