Volatile and molecular analysis of Juniperus brevifolia (Seub.) Antoine, an Azorean endemic species

Lima, A. Sofia; Trindade, Helena; Figueiredo, A. Cristina; Barroso, José G.; Pedro, Luís G.

doi:10.1016/j.bse.2010.07.010

Cited by 8 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Distinct ISSR primer sets applied in different investigations might also be one of the reasons in result bias: all seven primers used for J. brevifolia by Silva et al (2011) were the same as in our examination, while only three primers corresponded to the ones used in J. brevifolia survey (Bettencourt et al, 2015). Both (RAPD and ISSR) dominant markers showed rich allelic diversity in J. brevifolia accessions and a moderate genetic similarity (Lima et al, 2010). According to isoenzyme analysis of 35 populations of J. communis L. from Russia, only 12% interpopulation variability was observed in case of all chosen loci (10), at the same time, for separate two loci, 6-Pgdh-B and Fdh, 23 and 30% of genetic variability were distributed between populations (Hantemirova et al, 2012).…”

Section: Discussionmentioning

confidence: 70%

“…Genetic differences of juniper populations in northern and southern Lithuania might be partially related to different habitat sets in these parts of the country. In other studies, accessions of J. brevifolia (Lima et al, 2010) were also grouped according to their geographical origin. Both geographical and habitat elements might be underpinned in the studies of genetic diversity: edaphic, climatic and some other factors might affect genetic diversity of juniper populations of highland zone in Wales and chalk hills in South England located far away (Van der Merwe et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Caryocedrus, were compared by ISSRs (Adams et al, 2003). A few studies were devoted for evaluation of molecular diversity within juniper species: ISSR markers were applied for J. brevifolia (Lima et al, 2010;Silva et al, 2011;Bettencourt et al, 2015) and J. phoenicea populations (Meloni et al, 2006). Prior to our study, the only available information about J. communis obtained by RAPD markers came from Scotland (Adams, 2011) and Iran (Kasaian et al, 2011), while ISSR data for this species are still missing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity of Lithuanian populations of Juniperus communis L. in relation to abiotic and biotic factors

et al. 2016

View full text Add to dashboard Cite

Juniperus communis L. is increasingly threatened in many parts of Europe, including the Baltic region. Our present study was aimed at evaluation of genetic diversity of J. communis populations of Lithuania. Fourteen selected populations differed in geography and habitats (coastal brown dunes covered with natural Scots pine forests, further referred as B; J. communis shrubs, F; transition mires and quaking bogs, D; subcontinental moss Scots pine forests, G; xero-thermophile fringes, E). Molecular variance was analyzed among populations (140 individuals, in total), employing 14 Inter Simple Sequence Repeat (ISSR) markers. Percentage of polymorphic loci differed between populations belonging to the different habitat type, being the highest for population representing habitat F (68.2%) and the lowest for populations D (42.9%). The data demonstrate the possibility to underestimate or overestimate this parameter if some habitat populations are bypassed. Means per population of Nei's gene diversity and Shannon's information indexes, were 0.158 and 0.239, respectively. Significant correlation between genetic and geographic distance of populations of J. communis was documented by Mantel test. Bayesian analysis of ISSR data has separated populations of Southern Lithuania from Northern part. Juniperus communis populations representing different habitats showed moderate interpopulation variance at ISSR loci. In ISSR-based dendrograms, individuals were correctly allocated to populations, even in case of populations growing in 0.5 km vicinity (habitats D and G). Among all populations the most distinct one was representing habitat of J. communis shrubs (F) protected by EUNIS. Principal coordinate analysis of weighted averages of Ellenberg's indicator values (EIV) for herbaceous species, separated populations according to habitat type, while the same type analysis of ISSR data allowed distinguishing E habitat populations from populations of all other habitat type but G. Our study shows that on the local (Lithuanian) scale, molecular variance between populations of J. communis at ISSR loci might be related to habitat type. For evaluation and preservation of diversity of J. communis, different assessment methods should be included and management strategies should be directed in the way to retain variety of habitats encompassing both widely spread and less common ones.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of Lithuanian populations of Juniperus communis L. in relation to abiotic and biotic factors

et al. 2016

View full text Add to dashboard Cite

show abstract

“…A similar inference was also drawn [24] while analyzing the volatile constituents and molecular markers of Juniperus brevifolia. The DNAbased genetic similarity data do not predict chemical variability among the species of Sida complex, but DNA banding patterns are considered as reliable information toward the authentication and standardization of medicinal plant species [25].…”

Section: Resultsmentioning

confidence: 98%

Genetic and Chemical Diversity of High Mucilaginous Plants of Sida Complex by ISSR Markers and Chemical Fingerprinting

et al. 2011

View full text Add to dashboard Cite

A method was developed based on multiple approaches wherein DNA and chemical analysis was carried out toward differentiation of important species of Sida complex that is being used for commercial preparation. Isolated DNA samples were successfully performed through PCR amplification using ISSR markers and degree of genetic diversity among the different species of Sida is compared with that of chemical diversity. For genetic fingerprint investigation, selected 10 ISSR primers generating reproducible banding patterns were used. Among the total of 63 amplicons, 62 were recorded as polymorphic, genetic similarity index deduced from ISSR profiles ranged from 12 to 51%. Based on similarity index, S. acuta and S. rhombifolia found to be most similar (51%). High number of species-specific bands played pivotal role to delineate species at genetic level. Investigation based on HPTLC fingerprints analysis revealed 23 bands representing to characteristic chemicals and similarity index ranged from 73 to 91%. Prominent distinguishable bands were observed only in S. acuta, while S. cordifolia and S. rhombifolia shared most bands making them difficult to identify on chemical fingerprint basis. This report summarizes the genotypic and chemotypic diversity and the use of profiles for authentication of species of Sida complex.

show abstract

“…Juniperus species are substantial elements of present-day forests (Garcia et al, 2000;Adams, 2011) and some of them are on the border of extinction (Lima et al, 2010;Rumeu et al, 2011;Silva et al, 2011). This historically old genera is important for providing information about evolution of plants in Late Glacial period (Koller et al, 2005;Li et al, 2011, Veski et al, 2012.…”

Section: Introductionmentioning

confidence: 99%

Variety of habitats associated with common juniper (Juniperus communis L.) growing in Lithuania

Vilčinskas¹,

Marozas²,

Jocienė³

et al. 2016

biologija

View full text Add to dashboard Cite

Common juniper (Juniperus communis L.) is an important conifer from the ecological and economical point of view. In some areas of the distribution range, it is a widely studied species. Up till now insufficient attention has been paid to junipers growing in the Baltic countries where it is one of three natural conifers and important forest tree species. Recently, molecular markers-based diversity of Lithuanian populations of J. communis was determined, although related biological and ecological variables remain undisclosed. The present study is aimed at the evaluation of biotic and abiotic environment of J. communis according to plant species composition. Fourteen sites with J. communis were selected in the territory of Lithuania. At each site undergrowth trees and shrubs, herbaceous species, dwarf shrubs, and mosses were recorded and the abundance of each species was evaluated. Abiotic environment characteristics were obtained using Ellenberg's indicator values (EIV) of the plant species neighbouring junipers. In the sites with J. communis, 80 species of herbs and dwarf shrubs belonging to 18 families were registered. The largest variety of herbaceous species and dwarf shrubs (18-22 per site) was registered for sites representing xero-thermophile fringes. Species coverage data recorded in the sites in most cases allowed to group sites according to their habitats. Despite rather small territory of the present survey (latitude (N) 56°07'-54°11' , longitude (E) 21°06'-26°30'), the ranges of indexes for environment factors of juniper sites were rather wide: 6.0-7.3 for light, 3.0-5.8 for temperature, 3.7-5.3 for continentality, 4.2-7.8 for soil moisture, 2.7-7.4 for soil reaction, and 2.7-4.4 for soil nitrogen. Among the sites selected by us, the most contrasting in terms of EIV were locations representing coastal brown dunes covered with natural Scots pine forests and xero-thermophile fringes. Keywords INTRODUCTIONJuniperus species are substantial elements of present-day forests (Garcia et al., 2000;Adams, 2011) and some of them are on the border of extinction (Lima et al., 2010;Rumeu et al., 2011;Silva et al., 2011). This historically old genera is important for providing information about evolution of plants in Late Glacial period (Koller et al., 2005;Li et al., 2011, Veski et al., 2012. Juniperus contains economically valuable species used for medicinal purposes (Muto et al., 2008;Kusari et al., 2009), perfume industry (Lawrence, 1984, flavoring of food and alcoholic beverages (Maarse, 1991;Vichi et al., 2007). These circumstances point out the need of comprehensive knowledge about Juniperus species.Juniperus communis is widely distributed over the Northern hemisphere, growing in Europe and three other continents. Junipers have been examined by molecular and biochemical markers in various regions of their natural occurrence (Canary Islands, Greece, Germany, UK, Russia, Japan, China, Kazakhstan, Nepal, Ethiopia, Guatemala, Cuba, Mexico, Utah, Texas, etc. (Adams et al., 2003;Adams, 2011). Examination of variabl...

show abstract

Volatile and molecular analysis of Juniperus brevifolia (Seub.) Antoine, an Azorean endemic species

Cited by 8 publications

References 18 publications

Genetic diversity of Lithuanian populations of Juniperus communis L. in relation to abiotic and biotic factors

Genetic diversity of Lithuanian populations of Juniperus communis L. in relation to abiotic and biotic factors

Genetic and Chemical Diversity of High Mucilaginous Plants of Sida Complex by ISSR Markers and Chemical Fingerprinting

Variety of habitats associated with common juniper (Juniperus communis L.) growing in Lithuania

Contact Info

Product

Resources

About