Volatile compounds from in vitro metabolism of seven Listeria monocytogenes isolates belonging to different clonal complexes

Balsalobre, Esperanza Lepe; Rubio-Sánchez, Ricardo; Úbeda, Cristina; Lepe, José Antonio Suárez

doi:10.1099/jmm.0.001553

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…This effect was observed when studying the sorption of the equilibrium gas phase of highly dilute solutions of volatile substances (less than 0.01% vol.). The compounds selected to determine the detection limits were the most widely known volatile markers associated with the presence of pathogenic microorganisms in milk [ 62 , 63 , 64 , 65 , 66 , 67 ]. In relation to the other volatile compounds, the array of sensors was characterized by a high specific mass sensitivity of microweighing ( Table S1 ); the selected compounds were also present in the gas phase of milk, as in the natural changes associated with the proliferation of microorganisms during storage [ 61 , 62 ] and with the changes associated with the technological process of milk processing [ 68 , 69 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

Possibilities of an Electronic Nose on Piezoelectric Sensors with Polycomposite Coatings to Investigate the Microbiological Indicators of Milk

Shuba,

Umarkhanov,

Bogdanova

et al. 2024

Sensors

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Milk and dairy products are included in the list of the Food Security Doctrine and are of paramount importance in the diet of the human population. At the same time, the presence of many macro- and microcomponents in milk, as available sources of carbon and energy, as well as the high activity of water, cause the rapid development of native and pathogen microorganisms in it. The goal of the work was to assess the possibility of using an array of gas chemical sensors based on piezoquartz microbalances with polycomposite coatings to assess the microbiological indicators of milk quality and to compare the microflora of milk samples. Piezosensors with polycomposite coatings with high sensitivity to volatile compounds were obtained. The gas phase of raw milk was analyzed using the sensors; in parallel, the physicochemical and microbiological parameters were determined for these samples, and species identification of the microorganisms was carried out for the isolated microorganisms in milk. The most informative output data of the sensor array for the assessment of microbiological indicators were established. Regression models were constructed to predict the quantity of microorganisms in milk samples based on the informative sensors’ data with an error of no more than 17%. The limit of determination of QMAFAnM in milk was 243 ± 174 CFU/cm3. Ways to improve the accuracy and specificity of the determination of microorganisms in milk samples were proposed.

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Section: Discussionmentioning

confidence: 99%

Possibilities of an Electronic Nose on Piezoelectric Sensors with Polycomposite Coatings to Investigate the Microbiological Indicators of Milk

Shuba,

Umarkhanov,

Bogdanova

et al. 2024

Sensors

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Feasibility of using volatile urine fingerprints for the differentiation of sexually transmitted infections

Rubio-Sánchez,

Ubeda,

Ríos-Reina

2023

Appl Microbiol Biotechnol

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Sexually transmitted infections (STIs) are a public health problem worldwide, and current diagnostic methods have certain limitations. In recent years, volatile organic compounds (VOCs) have been studied as an alternative diagnostic method. Due to this, this study aimed to detect, in vaginal swabs and urine samples, VOCs emitted by highly prevalent STIs-causing bacteria (Chlamydia trachomatis, Mycoplasma genitalium, and Neisseria gonorrhoeae) to identify potential biomarkers that allow the detection of these STIs. VOCs detected in urine samples showed a better differentiation of patients with STIs due to C. trachomatis from those not infected, with 2,6-dimethyl-4-heptanone as the volatile compound most related to the presence of this bacterium. Among the VOCs most related to M. genitalium in urine, 4-methyltetradecane and 2-methylpentadecane stood out, while 3,4,4-trimethyl-2-cyclohexen-1-one was the VOC most closely related to N. gonorrhoeae infection. Moreover, C12 alcohols were the main VOC family associated with positive samples in all three bacteria, which could indicate the presence of aldehyde reductases in their metabolism. In contrast, alcohols such as 3-methyl-1-heptanol and 1-octanol, as well as dimethyl esters, were more associated with negative samples and may be useful in ruling out an STI caused by one of these three bacteria. In short, the VOCs identified as potential biomarkers in patients with infection by C. trachomatis, M. genitalium, or N. gonorrhoeae could be used in the early diagnosis of these STIs, quickly interrupting the chain of transmission, especially interesting in asymptomatic patients. Key points • Sexually transmitted infections are a serious public health problem worldwide. • The study of VOCs in multiple infections is increasing in recent years. • The identification of volatile biomarkers could allow new diagnostic methods. Graphical abstract

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Volatile biomarkers of Gram-positive bacteria of clinical relevance as a tool for infection diagnosis

Rubio-Sánchez,

Lepe-Balsalobre,

Ubeda

et al. 2024

Int Microbiol

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Aim Volatile organic compounds (VOCs) are being studied as potential biomarkers in many infections. Therefore, this study aimed to analyze the volatile profile of three Gram-positive bacteria of clinical relevance to identify potential volatile biomarkers that allow their differentiation. Methods and results L. monocytogenes, S. aureus, and E. faecalis clinical isolates were inoculated in a thioglycollate medium until grown. Then, VOCs were extracted by solid-phase microextraction, and the data obtained were subjected to multivariate analysis. According to our results, there was a high production of aldehydes in E. faecalis. In the case of alcohols, they only increased in L. monocytogenes, while ketones were produced significantly in all three bacteria, mainly due to acetoin. Acids were produced significantly in E. faecalis and L. monocytogenes. Conclusions Potential biomarkers of L. monocytogenes could be 1-butanol and 2-methylbutanoic acid. In the case of E. faecalis, the VOC most related to its presence was nonanal. Lastly, potential biomarkers of S. aureus could be isoamyl butanoate and methionol, although some pyrazines have also been associated with this bacterium. Significance and impact of the study The identification of potential biomarkers of these clinically relevant bacteria could open the way for the diagnosis of these infections through the analysis of volatile compounds.

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Volatile compounds from in vitro metabolism of seven Listeria monocytogenes isolates belonging to different clonal complexes

Cited by 3 publications

References 28 publications

Possibilities of an Electronic Nose on Piezoelectric Sensors with Polycomposite Coatings to Investigate the Microbiological Indicators of Milk

Possibilities of an Electronic Nose on Piezoelectric Sensors with Polycomposite Coatings to Investigate the Microbiological Indicators of Milk

Feasibility of using volatile urine fingerprints for the differentiation of sexually transmitted infections

Volatile biomarkers of Gram-positive bacteria of clinical relevance as a tool for infection diagnosis

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