Volatile Constituents and Anti-Osteoporotic Activity of the n-Hexane Extract From Homalomena gigantea Rhizome

Abstract

This study analyzed the chemical composition and anti-osteoporosis activity of the n-hexane extract of Homalomena gigantea rhizome. Sixty compounds, representing 92.0% of the extract, were identified by gas chromatography-mass spectrometry. Linalool (15.3%), oplopanone (9.8%), ( Ε)-α-atlantone (5.6%), khusinol acetate (5.4%), bullatantriol (4.3%), and β-sitosterol (3.8%) were the main constituents. The anti-osteoporotic activity of the n-hexane extract was determined by measuring alkaline phosphatase (ALP) activity, collagen content, and the mineralization of MC3T3-E1 cells. At concentrations of 4.0 and 20.0 µg/mL, the n-hexane extract increased ALP activity by 8.2% and 23.7%, and increased collagen secretion by MC3T3-E1 cells by 114.9% and 112.4%, respectively. At 4 µg/mL, the extract significantly promoted the mineralization of MC3T3-E1 cells by as much as 133.2% compared to the negative control. These results suggested that H. gigantea rhizome contains a natural anti-osteoporotic compound.

Essential Oil from Vietnamese Peperomia leptostachya Hook. & Arn. (Piperaceae): Chemical Composition, Antioxidant, Anti-Inflammatory, Cytotoxic Activities, and In Silico Analysis

“…The GC-MS analysis was conducted using a Shimadzu Technologies GCMS-QP2010 Plus chromatograph (Shimadzu, Kyoto, Japan), equipped with a fused silica Equity-5 capillary column (30 m × 0.25 mm, film thickness 0.25 µm, Supelco, Bellefonte, PA, USA) [ 38 , 39 , 40 , 41 ]. The analytical parameters were set as follows: helium was utilized as the carrier gas at a flow rate of 1.2 mL/min, while the injector and interface temperatures were maintained at 280 °C.…”

“…The GC-MS analysis was conducted using a Shimadzu Technologies GCMS-QP2010 Plus chromatograph (Shimadzu, Kyoto, Japan), equipped with a fused silica Equity-5 capillary column (30 m × 0.25 mm, film thickness 0.25 µm, Supelco, Bellefonte, PA, USA) [38][39][40][41]. The analytical parameters were set as follows: helium was utilized as the carrier gas at a flow rate of 1.2 mL/min, while the injector and interface temperatures were maintained at 280 • C. The temperature program entailed a ramp from 60 • C (held for 3 min) to 240 • C (held for 15 min) at a rate of 3 • C/min, followed by a final ramp to 280 • C (held for 35 min) at a rate of 5 • C/min for the column.…”

Volatile Organic Compounds and Antioxidant, Cytotoxic Activities of Extracts from the Leaves of <i>Grewia bulot</i>

“…The compounds were determined by comparing their mass spectra with those in Wiley 7 and NIST 11 libraries from the GC-MS system, as well as relevant literature data. Data quantification of the constituents was determined by the relative peak area [24].…”

GC-MS Characterization, in Vitro Antioxidant and Anti-Inflammatory Activities of Essential oil from the Leaves of Litsea balansae Lecomte

“…The GC-MS analysis was conducted using a Shimadzu Technologies GCMS-QP2010 Plus chromatograph (Shimadzu, Kyoto, Japan) equipped with a fused silica Equity-5 capillary column (30 m × 0.25 mm, film thickness 0.25 µm, Supelco). 26,27,28,29 The analytical parameters were set as follows: Helium was used as the carrier gas at a flow rate of 1.5 mL/min, with injector and interface temperatures set at 280 °C. The temperature program involved a ramp from 60 °C (held for 2 min) to 240 °C (held for 10 min) at a rate of 3 °C/min, followed by a final ramp to 280 °C (held for 40 min) at a rate of 5 °C/min for the column.…”