Volatile Constituents of Murraya koenigii Fresh Leaves Using Headspace Solid Phase Microextraction – Gas Chromatography – Mass Spectrometry

Abstract

The volatile components of Murraya koenigii fresh leaves, collected from Surat Thani province, Thailand were studied by using headspace (HS) solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The four fibers employed to extract the volatiles were polydimethylsiloxane (PDMS), polydimethylsiloxane-divinylbenzene (PDMS-DVB), carboxane-polydimethylsiloxane (CAR-PDMS) and polydimethylsiloxane-divinylbenzene-carboxane (PDMS-DVB-CAR). The volatile constituents of M. koenigii fresh leaves were also extracted by hydrodistillation and analyzed by GC-MS. Fifty-one compounds were identified by these fibers. Five major compounds, -terpinene, -caryophyllene, -phellandrene, -selinene and -pinene, were detected in all fibers. The PDMS-DVB-CAR fiber was considered as the best for trapping key volatiles of M. koenigii fresh leaves.

Phytochemical Analysis and Evaluation of Antioxidant and Biological Activities of Extracts from Three Clauseneae Plants in Northern Thailand

“…According to the results of the assessment of the essential oil of M. koenigii , it was found to possess Anethole (26.02%), Caryophyllene (21.15%) and α- Pinene (12.23%) as the major components. These results are in accordance with those presented in the published report of Sukkaew et al [ 27 ], wherein the essential oil obtained from the fresh leaves of M. koenigii collected from Surat Thani Province, Thailand, was found to contain β- Caryophyllene (21.4%), α- Selinene (10.2%) and α- Humulene (7.1%) and α- Pinene (4.4%) as its major components. However, in this study, the chemical composition of the essential oil of M. koenigii was distinctly different from that of previous studies.…”

Comparison of Volatile Organic Compounds of Sideritis romana L. and Sideritis montana L. from Croatia

“…In this study, DVB/CAR/PDMS and PDMS/DVB fiber, which were previously shown to be most suitable for untargeted HS-SPME analysis of volatiles [ 35 ], were used for the analysis of VOCs from aerial parts of S. romana and S. montana from Croatia. In our study, the number of extracted VOCs was higher using DVB/CAR/PDMS fiber (69 compounds) than it was by using PDMS/DVB fiber (56 compounds), which is in accordance with the work of Wang et al [ 37 ], in which DVB/CAR/PDMS fiber showed better efficiency to extract volatile compounds (50 compounds) from the samples of Aquilegia japonica Nakai and H.Hara, compared to CAR/PDMS (47 compounds) and PDMS/DVB fibers (45 compounds), as well as with the work of Sukkaew et al [ 38 ], where more volatile components were extracted using DVB/CAR/PDMS fiber (51 compounds) than with PDMS/DVB (38 compounds), PDMS (38 compounds) and CAR/PDMS (37 compounds) fibers in Murraya koenigii (L.) Sprengel fresh leaves. In our study, the dominant chemical classes of volatile compounds in all samples of the two investigated Sideritis species extracted using DVB/CAR/PDMS and PDMS/DVB fibers were sesquiterpene hydrocarbons followed by monoterpene hydrocarbons, except for S. romana M in whose extract monoterpene hydrocarbons were more abundantly present.…”

Characterization of the Major Odor-Active Compounds in the Leaves of the Curry Tree Bergera koenigii L. by Aroma Extract Dilution Analysis

“…12,14,16 A very recent study is so far the only one that aimed at identifying the volatiles not only in the hydrodistilled essential oil but also in the fresh leaves. 18 Using solid phase microextraction, 51 compounds, almost exclusively (sesqui)terpenes and (sesqui)terpenoids, were detected in the headspace above freshly picked leaf material.…”