Volatiles mediated an eco‑friendly aphid control strategy of Chrysanthemum genus

Zhong, Jian; Guo, Yanhong; Shi, Huajin; Liang, Yilin; Guo, Ziyu; Li, Dawei; Wang, Chen; Hai, Li; Zhang, Qixiang; Sun, Ming

doi:10.1016/j.indcrop.2022.114734

Cited by 12 publications

(6 citation statements)

References 41 publications

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“…These findings are important for further clarifying the mechanism underlying volatile biosynthesis in daffodils. The MVA and MEP pathways are two different metabolic pathways for terpene biosynthesis ( Nagegowda, 2010 ; Zhong et al., 2022 ). Both pathways are active in O. longilobus leaves, buds, and inflorescences at different developmental stages.…”

Section: Discussionmentioning

confidence: 99%

“…Terpenoid synthetase catalyzes the conversion of the corresponding precursor compounds into various terpenoids. Isoprene, which is the basic component of terpenoids, is an important raw material for the synthesis of rubber, pesticides, medicines, spices, and adhesives ( Zhong et al., 2022 ). In the current study, we analyzed the gene regulatory network of terpenoids in O. longilobus .…”

Section: Discussionmentioning

confidence: 99%

“…They can be divided into semi-terpenes, monoterpenes, sesquiterpenes, diterpenes, triterpenes, tetraterpenes, polyterpenes, and irregular terpenes ( He et al., 2020 ). Terpenes consist of several isoprene (C5) units, and they are synthesized by a complex network regulated by multiple genes and enzymes ( Zhong et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…One is the mevalonate (MVA) pathway in the cytoplasm and mitochondria, wherein it is involved in the biosynthesis of secondary metabolites, including sterols, sesquiterpenes, and triterpenes. Another is the plastid-based 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, which is mainly involved in the biosynthesis of monoterpenes, diterpenes, and carotenoids ( Nagegowda, 2010 ; Zhong et al., 2022 ). There has been considerable progress in the research on the biochemical pathways and molecular mechanisms regulating floral volatiles ( Wong et al., 2017 ; Fan et al., 2018 ; Ramya et al., 2018 ), but because of the lack of relevant data, the metabolism of aromatic substances in O. longilobus leaves and inflorescences remains a mystery.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus

et al. 2022

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Opisthopappus longilobus, which is a unique wild plant resource in China, produces leaves and flowers with distinct aromas. However, there have been relatively few molecular studies on its floral aroma, which has hindered the research on this plant species at the molecular level and the breeding of novel varieties. In this study, transcriptome and metabolome analyses were performed using O. longilobus leaves, buds, and inflorescences at the exposure, initial opening, and blooming stages. Using high-quality reads and assembly software, a total of 45,674 unigenes were annotated according to the Nr, Swiss-Prot, KOG, and KEGG databases. Additionally, a GC-MS system and a self-built database were used to detect 1,371 metabolites in the leaves, buds, and inflorescences. Terpene metabolites were the most common compounds (308 in total). We analyzed the gene network regulating terpenoid accumulation in O. longilobus and identified 56 candidate genes related to terpenoid synthesis. The expression of OlPMK2, OlMVK1, OlTPS1, and OlTPS3 may lead to the accumulation of 11 different terpenoids specifically in the inflorescences at the exposure, initial opening, and blooming stages. The generated data may be useful for future research on O. longilobus genetic resources and the molecular mechanism regulating aroma formation in this plant species. The findings of this study may be used to accelerate the breeding of new O. longilobus varieties with enhanced aromatic traits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus

et al. 2022

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“…However, a limitation of this approach is that the successful combination of all desired traits within a single cultivar relies more on chance than on a deliberate strategy. Most cultivated chrysanthemums are highly susceptible to aphid infestations, resulting in significant economic losses and the excessive use of toxic pesticides as a primary control measure ( Saicharan et al., 2019 ; Zhong et al., 2022 ). Chrysanthemums are chosen as a host plant by aphids due to their distinctive color and the presence of volatile compounds ( Guo et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Aphid alarm pheromone mimicry in transgenic Chrysanthemum morifolium: insights into the potential of (E)-β-farnesene for aphid resistance

Li,

Hu,

Ren

et al. 2024

Front. Plant Sci.

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(E)-β-Farnesene (EBF) serves as the primary component of the alarm pheromone used by most aphid pest species. Pyrethrum (Tanacetum cinerariifolium) exhibits tissue-specific regulation of EBF accumulation and release, effectively mimicking the aphid alarm signal, deterring aphid attacks while attracting aphid predators. However, cultivated chrysanthemum (Chrysanthemum morifolium), a popular and economically significant flower, is highly vulnerable to aphid infestations. In this study, we investigated the high expression of the pyrethrum EBF synthase (TcEbFS) gene promoter in the flower head and stem, particularly in the parenchyma cells. Subsequently, we introduced the TcEbFS gene, under the control of its native promoter, into cultivated chrysanthemum. This genetic modification led to increased EBF accumulation in the flower stem and young flower bud, which are the most susceptible tissues to aphid attacks. Analysis revealed that aphids feeding on transgenic chrysanthemum exhibited prolonged probing times and extended salivation durations during the phloem phase, indicating that EBF in the cortex cells hindered their host-location behavior. Interestingly, the heightened emission of EBF was only observed in transgenic chrysanthemum flowers after mechanical damage. Furthermore, we explored the potential of this transgenic chrysanthemum for aphid resistance by comparing the spatial distribution and storage of terpene volatiles in different organs and tissues of pyrethrum and chrysanthemum. This study provides valuable insights into future trials aiming for a more accurate replication of alarm pheromone release in plants. It highlights the complexities of utilizing EBF for aphid resistance in cultivated chrysanthemum and calls for further investigations to enhance our understanding of this defense mechanism.

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Identification and functional analysis of terpene synthases revealing the secrets of aroma formation in Chrysanthemum aromaticum

Zhong,

Chen,

Shi

et al. 2024

International Journal of Biological Macromolecules

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Volatiles mediated an eco‑friendly aphid control strategy of Chrysanthemum genus

Cited by 12 publications

References 41 publications

Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus

Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus

Aphid alarm pheromone mimicry in transgenic Chrysanthemum morifolium: insights into the potential of (E)-β-farnesene for aphid resistance

Identification and functional analysis of terpene synthases revealing the secrets of aroma formation in Chrysanthemum aromaticum

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