Vollautomatische Herstellung von Knochenschliffen bei exakter Dickeneinstellung

Abstract

A new grinding machine for preparing thin undecalcified bone sections after methylmethacrylate embedding is described. About 20 rather small bone sections can be ground at the same time; bigger specimens, up to 8 cm of length, are allowed. Bone sections are mounted on a cylindrical specimen holder by an adhesive film. Then the final thickness of the sections is exactly adjusted by screwing three rubies out of the holder’s bottom. Now the prepared holder is set in a guide ring on a turntable carrying a rough ended glass plate. The desired thickness of the sections is reached as soon as the three rubies touch the glass surface. The variation in the thickness of the sections is less than ± 3 μm. The machine is simply constructed, easily to handle and rapidly to clean.

Autologous osteoblasts enhance osseointegration of porous titanium implants

“…In order to determine the density, quality, and quantity of the ingrown bone, microradiography of each histological section was performed on a faxitron X-ray system (Hewlett Packard, San Diego, USA) with high-resolution plates (INTAS, Gottingen, Germany) [35]. Plates were analyzed by means of a light microscope and the Quantimet system (Leica DM-RXE).…”

“…The saw blade itself had a thickness of 300 pm. The implant sections were then prepared on slides for further analysis [35].…”

New Uses of Plastination in Bone Histology

“…It is possible to prepare thin sections using a modern microgrinding system with vacuumhold (10). Thus, morphomctric investigations arc also possible.…”