Voltammetric analysis of disulfiram in pharmaceuticals with a cyclic renewable silver amalgam film electrode

Abstract

Electrochemical properties of disulfiram, representative of highly significant bioactive compounds, were studied with a cyclic renewable silver amalgam film electrode (Hg(Ag)FE) using square wave cathodic stripping voltammetry (SWCSV). The influence of various factors such as pH, buffer concentration, buffer composition, and SWCSV parameters on current response was investigated. The optimum results in terms of signal shape and intensity were recorded in Britton-Robinson buffer (pH 7.5) at -0.5 V versus Ag/AgCl/3 mol L −1 KCl. An elaborated electroanalytical procedure was used to determine disulfiram at the Hg(Ag)FE in the concentration range from 0.05 to 5.00 µ M. Precision, repeatability, and accuracy of the method as well as the influence of possible interferences were ascertained. The detection and quantification limits were 11 nM and 37 nM, respectively. The applicability of the developed method was tested in the determination of disulfiram in the commercial formulation Anticol. Thin-layer chromatography with image processing software was used to validate the accuracy of the method.

Optimization of Square Wave Voltammetry (SWV) Parameters by Design of Experiment (DoE) Methodologies for the Determination of Mifepristone at Glassy Carbon Electrode (GCE)

“…As compared with other electroanalytical techniques, SWV offers advantages of speed, improved analytical sensitivity and often allowing direct detection of toxic metals and organic pollutants down to pico-molar concentration found in the environmental samples [4][5][6][7][8][9][10][11][12][13]. Besides these major (excellent) advantages, the performance of SWV is influenced by several parameters such as pH, frequency, potential, pulse amplitude, and scan increment, among others [3,14,15]. This set of operating parameters are often optimized by a univariate approach called one-at-atime (OAAT) optimization process, where only one parameter is varied at different values while the other parameters are kept at constant level.…”

Lactofen – Electrochemical Sensing and Interaction with dsDNA

“…The evaluated detection and quantitation limits were 0.28 mM and 0.95 mM, respectively. As these result were unsatisfactory (due to high LOD, LOQ values and narrow linear concentration range) and introduction of stripping step did not give any improvement, we decided to check whether better analytical characteristics might be obtained with the silver amalgam film electrode, as many literature positions report excellent usefulness of this electrode in biologically active compounds determination [1,[19][20][21].…”