Voltammetric Behavior of the Alizarin Red S Interaction with DNA and Damage to DNA

Abstract

The electrochemical behavior and the interaction of alizarin red S (ARS) with calf thymus DNA was investigated on a bare glassy carbon electrode (GCE) and DNA modified GCE (DNA/GCE), respectively. ARS showed a pair of redox peaks at À 0.445 V and À 0.414 V on a bare GCE. On addition of DNA into the ARS solution, the peak current of ARS decreased and the peak potential positively shifted, but without new redox peaks appeared. The ARS reduction peak current increased with immersion time on a DNA/GCE. The results showed that ARS could interact with DNA molecules by intercalative binding mode. The equilibrium constant, binding number and the ratio of binding constant for oxidized and reduced ARS forms were obtained. The DNA damage was directly detected by appearance of guanosine and adenosine bases oxidation signal. The influence of experimental conditions on DNA damage extent was discussed in detail.

Association Behavior of 3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic Acid Sodium Salt in Cationic Surfactant Medium Under Different pH Conditions

“…[9] Certain voltammetric studies show that ARS can cause damage to DNA. [10,11] ARS has a tremendous potential for use in metal ions detection due to its high sensitivity for different metals. [4,5,[12][13][14][15][16][17][18][19] At the same time, ARS can form complexes with various metal ions [8,20,21] and can be used for their separation from waste waters.…”

Enhanced Sensing of 4-Nitroso-N,N–Dimethyl Aniline using Multi-Walled Carbon Nano Tube Modified Glassy Carbon Electrode

“…Nowadays, most of the pollutants have been determined in trace levels quantitatively using DPSV. The electro chemical behaviour and the interaction of dye with calf thymus DNA was investigated by Yang et al on a bare GCE and DNA modified GCE respectively [21].…”

A Novel Cysteine-Functionalized MxOy Material as Support for Laccase Immobilization and a Potential Application in Decolorization of Alizarin Red S

“…Many methods are used for the degradation and decolorization of dyes. These methods can be divided into three categories: physical methods (nano-filtration, reverse osmosis, electrodialysis) [35], sorption techniques (photochemical, electrochemical destruction) [36] and biological methods (enzymatic degradation) [37]. Biological methods have low running costs, produce stable and harmless final products, and also require fewer chemicals and less energy than physical and chemical methods.…”