Voltammetric Determination of 2′‐Deoxyadenosine and Adenine in Urine of Patients with Hepatocellular Carcinoma Using Fullerene‐C₆₀‐modified Glassy Carbon Electrode

Abstract

A fullerene-C 60 -modified glassy carbon electrode has been examined for the simultaneous determination of 2'-deoxyadenosine (2'-dAdo) and adenine in human blood and urine using Osteryoung square-wave voltammetry (OSWV) at pH 7.2. Compared to bare glassy carbon electrode (GCE), the modified electrode displays a shift of the oxidation potential in the negative direction with significant increase in the peak current for both the analytes. At modified electrode well-defined anodic peaks at potential of 1248 mV and 994 mV are observed for 2'-dAdo and adenine respectively. Linear calibration curves were obtained within the concentration range 10 nM to 100 mM for both the compounds in 0.1 M phosphate buffer solution (PBS) with the limit of detection 0.8 Â 10 À8 M and 0.95 Â 10 À8M for 2'-dAdo and adenine respectively. The analytical utility of the present method is demonstrated by quantitative detection of 2'-dAdo and adenine in human urine of normal subjects as well as in patients with hepatocellular carcinoma. Interfering effect of some coexisting metabolites has also been reported.

Synthesis, crystal structure and electrocatalytic behavior of a copper (II) complex derived from acylpyrazolone double Schiff bases

“…The slope of 15mV/pH also indicated that two protons took part in the rate‐determining step . This result was in agreement with the electrooxidation mechanism reported . The pH values around 5.5 should provide the highest oxidation currents justifying the selection of pH 5.5 for the following experiments using the modified electrode.…”

Copper Oxide Nanoparticle Modified Sol–Gel‐Derived Carbon Ceramic by Microwave Irradiation, and Its Application for Determination of Adenine at Very Low Potential

“…The results show that by increasing adsorption time, the I p is increased, but for saving time, 3 min adsorption time was used for the rest of experiments. In contrast to our previous report [36], in which the adsorbed adenine could be removed easily by applying some CVs in supporting electrolyte, in this study adenine adsorbs so strongly that even by applying 10 CVs on the electrode surface (0 to 0.5 V), adenine peaks still remains. However when these sweeps were applied in an alkaline solution or the potential range of the sweeps in PBS7.3 were wider (À0.7 to 1 V or 0 to 1 V), the peaks disappeared, but a little change in the background current was observed, and such cleaning causes a decrease in the response of adenine.…”

“…As it is known, adenine is an integral part of DNA, RNA and adenosine triphosphate (ATP), which serves as an energy storage molecule and is used to power many chemical reactions within the cell [34][35][36].…”

Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode

“…They are very important in storing genetic information. Measuring the levels of A and G is important in bioscience and clinical diagnosis, because their quantities can act as important indicators for the diagnosis of various illnesses [ 1 , 2 , 3 , 4 , 5 ].…”