Voltammetric determination of glutathione in haemolysed erythrocyte and tablet samples using modified‐multiwall carbon nanotubes paste electrode

Abstract

A chemically modified electrode was prepared by incorporating p-aminophenol into multiwall carbon nanotubes paste matrix. Cyclic voltammetry, square wave voltammetry, double potential step chronoamperometry, and electrochemical impedance spectroscopy were used to investigate the electrochemical behaviour of glutathione at the chemically modified electrode prepared. According to the results, p-aminophenol multiwall carbon nanotubes paste electrode (p-APMWCNTPE) showed high electrocatalytic activity for glutathione oxidation, producing a sharp oxidation peak current at about +0.285 vs Ag/AgCl reference electrode at pH 5.0. Chronoamperometry was also used to determine glutathione's catalytic rate constant and diffusion coefficient at p-APMWCNTPE. The square wave voltammetric peak current of glutathione increased linearly with glutathione concentration in the range of 2.0 × 10(-7) - 1.0 × 10(-4) mol L(-1) with a detection limit of 9.0 × 10(-8) mol L(-1). The method was also successfully employed as a selective, simple, and precise method for the determination of glutathione in haemolysed erythrocyte, tablet, and urine samples.

Optimization of determination of reduced and oxidized glutathione in rat striatum by HPLC method with fluorescence detection and pre-column derivatization

“…There are many analytical methods (i.e., colorimetric, spectrofluorometric, and chromatographic) used to determine GSH and GSSG in various biological samples [10][11][12][13].…”

A Differential Pulse Voltammetric Sensor for Determination of Glutathione in Real Samples Using a Trichloro(terpyridine)ruthenium(III)/Multiwall Carbon Nanotubes Modified Paste Electrode

“…However, most of these methods suffer from difficulties of sample preparation, the necessity for derivatization, or lack of sufficient sensitivity, all of which limit their utility. Compared with the other techniques, electrochemical methods [15][16][17][18] have shown remarkable advantages in the analysis of different compounds in real samples. These advantages are mainly due to the simplicity, low cost, and relatively short analysis times of these compounds as compared to the chromatography methods [19][20][21][22][23][24][25][26].…”

A novel biosensor for liquid phase determination of glutathione and amoxicillin in biological and pharmaceutical samples using a ZnO/CNTs nanocomposite/catechol derivative modified electrode

“…Thus, the development of a simple, precise and accurate procedure for the determination of this drug in pharmaceutical products is very useful. Many methods have been so far reported for its determination that include titrimetry [3], spectrophotometry [4], high performance liquid chromatography [5], capillary electrophoresis [6], fluorimetry [7], and electrochemical [8][9][10][11][12][13][14][15][16] methods.…”