Voltammetric determination of lactate dehydrogenase using a carbon paste electrode

Tărmure, C; Săndulescu, Robert; Ionescu, Clara M.

doi:10.1016/s0731-7085(99)00278-2

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…The peak current of NADH is linearly related to the LDH concentration and hence can be determined voltammetrically. [140] Anti-LDH antibody immobilized GCE modified with MWCNT and gold nanoparticle was reported to be efficient sensor for determining LDH in the range 0.001 μg/mL to 0.5 μg/mL. DPV technique was used for analyzing the variation in NADH current and quantification of LDH.…”

Section: Cardiac Biomarker 3651 Cardiac Troponin (Ctn)mentioning

confidence: 99%

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Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

2023

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The recent events of outbreaks of corona virus caused by the Severe Acute Respiratory Syndrome Coronavirus −2(SARS‐CoV‐2) raised a concern and increased urge for quick and rapid detection techniques of disease causing pathogens. The detection of the virus with high sensitivity and accuracy holds great importance. Nowadays, the most commonly employed technique is the Quantitative Reverse Transcription Polymerase Chain Reaction test (qRT‐PCR) and antigen test. While RT‐PCR technique is time consuming, expensive and labour intensive, antigen tests though simple, can often give false positive and false negatives. In this context, electrochemical biosensors developed in recent times have been identified as a potential strategy to overcome the limitations of these common techniques. This review article summarizes the current advancements in the field of voltammetric sensors for the detection of COVID‐19 virus and various biomarkers associated with it.

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Section: Cardiac Biomarker 3651 Cardiac Troponin (Ctn)mentioning

confidence: 99%

“…The basic principle behind voltammetric determination of LDH is that it can catalyse lactate oxidation by NAD + resulting in NADH. The peak current of NADH is linearly related to the LDH concentration and hence can be determined voltammetrically [140] …”

Section: Voltammetric Sensors For Diagnosis Of Sars‐cov‐2 and Its Bio...mentioning

confidence: 99%

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

2023

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Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase Activity Indirectly

Zhang

Wang

Chang

et al. 2012

Methods in Molecular Biology

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Nanotoxicity assessment methods for nanoparticles (NPs) such as carbon nanotubes (CNTs), nano-Al(2)O(3), and tridecameric aluminum polycation or nanopolynuclear (nano-Al(13)), particularly lactate dehydrogenase (LDH) assays are reviewed. Our researches on electrochemically monitoring the variations of LDH activity indirectly in the presence of multiwalled carbon nanotubes (MWCNTs), nano-Al(13), and nano-Al(2)O(3) separately to derive toxic concentrations of NPs altering LDH activity by 50% (TC(50)) values are discussed. TC(50) values indicated that the toxicity order was Al (III)> MWCNTs > nano-Al(13) > nano-Al(2)O(3). Zeta potentials (ζ) data of these NPs in the literature proved that the surfaces of these NPs are charged negatively. Negatively charged surfaces might be a main cause in the reduction of LDH activity. Therefore, the classic LDH assays are doubtful to underestimate the nanotoxicities when they are applied to those NPs with negatively charged surfaces. These observations highlight and reconcile some contradictory results at present such as medium-dependent toxicity of NPs among the literature and develop novel analytical methods for evaluation of toxicities of NPs.

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2012

Analytical Chemistry

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Voltammetric determination of lactate dehydrogenase using a carbon paste electrode

Cited by 3 publications

References 12 publications

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase Activity Indirectly

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