Voltammetric immunoassay for Mycobacterium tuberculosis secretory protein MPT64 based on a synergistic amplification strategy using rolling circle amplification and a gold electrode modified with graphene oxide, Fe3O4 and Pt nanoparticles

Gou, Dan; Li, Yuxia; Zhang, Xin; Chen, Hui

doi:10.1007/s00604-018-2972-6

Cited by 29 publications

(11 citation statements)

References 30 publications

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“…Several groups have used a combination of antibody-coated MPs to capture analytes followed by addition of an aptamer with a primer extension to allow RCA. 299,318,331 An example of this assay involved using immunomagnetic beads to isolate PDGF from a sample, followed by addition of the anti-PDGF aptamer to form a sandwich complex. A primer extension on the aptamer was then used to initiate exponential dendritic RCA and the resulting RP was then hybridized with GOx-labelled cDNA.…”

Section: Fna-based Assays and Poc Diagnostic Devices Utilizing Rcamentioning

confidence: 99%

Functional nucleic acid biosensors utilizing rolling circle amplification

Bialy

Mainguy

et al. 2022

Chem. Soc. Rev.

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Section: Fna-based Assays and Poc Diagnostic Devices Utilizing Rcamentioning

confidence: 99%

Functional nucleic acid biosensors utilizing rolling circle amplification

Bialy

Mainguy

et al. 2022

Chem. Soc. Rev.

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“…This emphasizes the need for a diagnostic method that detects only live bacilli. Some other new technologies for TB diagnosis have recently emerged, such as surface plasmon resonance spectroscopy [41], immuno-PCR (a combination of ELISA and PCR) [42,43], voltammetric assay [44,45], aptasensor [46,47], immuno-nanosensor [48], and electrochemiluminescence immunoassay [49]. These methods are also applied to secretory proteins (i.e., antigens) as TB markers, but culture, which takes time, is still required to obtain adequate amounts of antigen [50].…”

Section: Discussionmentioning

confidence: 99%

A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity

Wang

Takeuchi²,

Jain

et al. 2020

EBioMedicine

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Background Nucleic acid amplification tests (NAATs) are widely used to diagnose tuberculosis (TB), but cannot discriminate live bacilli from dead bacilli. Live bacilli can be isolated by culture methods, but this is time-consuming. We developed a de novo TB diagnostic method that detects only live bacilli with high sensitivity within hours. Methods A prospective study was performed in Taiwan from 2017 to 2018. Sputum was collected consecutively from 1102 patients with suspected TB infection. The sputum was pretreated and heated at 46°C for 1 h to induce the secretion of MPT64 protein from live Mycobacterium tuberculosis . MPT64 was detected with our ultrasensitive enzyme-linked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide (thio-NAD) cycling. We compared our data with those obtained using a culture test (MGIT), a smear test (Kinyoun staining), and a NAAT (Xpert). Findings The limit of detection for MPT64 in our culture-free ultrasensitive ELISA was 2.0 × 10 −19 moles/assay. When the criterion for a positive response was set as an absorbance value ≥17 mAbs, this value corresponded to ca . 330 CFU/mL in the culture method – almost the same high-detection sensitivity as the culture method. To confirm that MPT64 is secreted from only live bacilli, M. bovis BCG was killed using 8 μg/mL rifampicin and then heated. Following this procedure, our method detected no MPT64. Our rapid ultra-sensitive ELISA-based method required only 5 h to complete. Comparing the results of our method with those of culture tests for 944 specimens revealed a sensitivity of 86.9% (93/107, 95% CI: 79.0–92.7%) and a specificity of 92.0% (770/837, 95% CI: 89.9–93.7%). The performance data were not significantly different (McNemar's test, P = 0.887) from those of the Xpert tests. In addition, at a ≥1+ titer in the smear test, the positive predictive value of our culture-free ultrasensitive ELISA tests was in a good agreement with that of the culture tests. Furthermore, our culture-free ultrasensitive ELISA test had better validity for drug effectiveness examination than Xpert tests because our test detected only live bacilli. Interpretation Our culture-free ultrasensitive ELISA method detects only live TB bacilli with high sensitivity within hours, allowing for rapid diagnosis of TB and monitoring drug efficacy. Funding Matching Planner Program from JST (VP29117939087), the A-STEP Program from JST (AS3015096U), Waseda University grants for Specific Research Projects (2017A-015 and 2019C-123), the Precise Measurement Technology Promotion Foundation to E.I.

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“…For complete of discussion, Table 2 25‐206 summarized all of the discussed articles on the detection of bacterial. It is important to point out that, there is other reports in this research area …”

Section: Biosensing Based On Type Of Bacteriamentioning

confidence: 99%

Recent progress and challenges on the bioassay of pathogenic bacteria

2020

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The present review (containing 242 references) illustrates the importance and application of optical and electrochemical methods as well as their performance improvement using various methods for the detection of pathogenic bacteria. The application of advanced nanomaterials including hyper branched nanopolymers, carbon‐based materials and silver, gold and so on. nanoparticles for biosensing of pathogenic bacteria was also investigated. In addition, a summary of the applications of nanoparticle‐based electrochemical biosensors for the identification of pathogenic bacteria has been provided and their advantages, detriments and future development capabilities was argued. Therefore, the main focus in the present review is to investigate the role of nanomaterials in the development of biosensors for the detection of pathogenic bacteria. In addition, type of nanoparticles, analytes, methods of detection and injection, sensitivity, matrix and method of tagging are also argued in detail. As a result, we have collected electrochemical and optical biosensors designed to detect pathogenic bacteria, and argued outstanding features, research opportunities, potential and prospects for their development, according to recently published research articles.

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Voltammetric immunoassay for Mycobacterium tuberculosis secretory protein MPT64 based on a synergistic amplification strategy using rolling circle amplification and a gold electrode modified with graphene oxide, Fe3O4 and Pt nanoparticles

Cited by 29 publications

References 30 publications

Functional nucleic acid biosensors utilizing rolling circle amplification

Functional nucleic acid biosensors utilizing rolling circle amplification

A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity

Recent progress and challenges on the bioassay of pathogenic bacteria

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