Voltammetric in-situ monitoring of leuco-indigo in indigo-fermenting suspensions

Nakagawa, Kasumi; Takeuchi, Mitsuaki; Kikuchi, Mayu; Tada, Manami; Sakamoto, Takako; Kano, Kenji; Ogawa, Jun; Sakuradani, Eiji

doi:10.1016/j.jbiosc.2021.01.005

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…A fresh indigo-dye fermenting suspension was prepared following the same method employed in our previous studies [27,28]. Twenty grams of Sukumo was mixed with 20 mL of hot water.…”

Section: Preparation Of the Indigo-dye Fermenting Suspensionmentioning

confidence: 99%

“…Aizome (Japanese indigo dyeing) is a unique dyeing method involving microorganisms under anaerobic alkaline conditions [27,28]. The Japanese indigo plant, Polygonum tinctorium or Persicaria tinctoria, accumulates indican (indoxyl-β-D-glucoside), from which indigo is generated during the drying and oxidative fermentation of the leaves of the plant.…”

Section: Introductionmentioning

confidence: 99%

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Indigo-Mediated Semi-Microbial Biofuel Cell Using an Indigo-Dye Fermenting Suspension

et al. 2021

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Aizome (Japanese indigo dyeing) is a unique dyeing method using microbial activity under anaerobic alkaline conditions. In indigo-dye fermenting suspensions; microorganisms reduce indigo into leuco-indigo with acetaldehyde as a reductant. In this study; we constructed a semi-microbial biofuel cell using an indigo-dye fermenting suspension. Carbon fiber and Pt mesh were used as the anode and cathode materials, respectively. The open-circuit voltage (OCV) was 0.6 V, and the maximum output power was 32 µW cm−2 (320 mW m−2). In addition, the continuous stability was evaluated under given conditions starting with the highest power density; the power density rapidly decreased in 0.5 h due to the degradation of the anode. Conversely, at the OCV, the anode potential exhibited high stability for two days. However, the OCV decreased by approximately 80 mV after 2 d compared with the initial value, which was attributed to the performance degradation of the gas-diffusion-cathode system caused by the evaporation of the dispersion solution. This is the first study to construct a semi-microbial biofuel cell using an indigo-dye fermenting suspension.

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“…A fresh indigo-dye fermenting suspension was prepared following the same method employed in our previous studies [27,28]. Twenty grams of Sukumo was mixed with 20 mL of hot water.…”

Section: Preparation Of the Indigo-dye Fermenting Suspensionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Indigo-Mediated Semi-Microbial Biofuel Cell Using an Indigo-Dye Fermenting Suspension

et al. 2021

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Quantification of leuco-indigo in indigo-dye-fermenting suspension by normal pulse voltammetry

Kikuchi

Sowa

Takeuchi

et al. 2022

Journal of Bioscience and Bioengineering

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Isolation and characterization of indigo-reducing bacteria and analysis of microbiota from indigo fermentation suspensions

Nakagawa

Takeuchi

Tada

et al. 2021

Bioscience, Biotechnology, and Biochemistry

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In natural indigo dyeing, the water-insoluble indigo included in the composted indigo leaves called sukumo is converted to water-soluble leuco-indigo through the reduction activities of microorganisms under alkaline conditions. To understand the relationship between indigo reduction and microorganisms in indigo-fermentation suspensions, we isolated and identified the microorganisms that reduce indigo and analyzed the microbiota in indigo-fermentation suspensions. Indigo-reducing microorganisms, which were not isolated by means of a conventional indigo carmine-reduction assay method, were isolated by using indigo as a direct substrate and further identified and characterized. We succeeded in isolating bacteria closely related to Corynebacterium glutamicum, Chryseomicrobium aureum, Enterococcus sp. for the first time. Anthraquinone was found to be an effective mediator that facilitated the indigo-reduction activity of the isolated strains. On analysis of the microbiota in indigo-fermentation suspensions, the ratio of indigo-reducing bacteria and others was found to be important for maintaining the indigo-reduction activity.

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Voltammetric in-situ monitoring of leuco-indigo in indigo-fermenting suspensions

Cited by 4 publications

References 14 publications

Indigo-Mediated Semi-Microbial Biofuel Cell Using an Indigo-Dye Fermenting Suspension

Indigo-Mediated Semi-Microbial Biofuel Cell Using an Indigo-Dye Fermenting Suspension

Quantification of leuco-indigo in indigo-dye-fermenting suspension by normal pulse voltammetry

Isolation and characterization of indigo-reducing bacteria and analysis of microbiota from indigo fermentation suspensions

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