Voltammetric investigation of complex growth media at a bare glassy carbon electrode: A case study of oxytetracycline

Kruid, Jan; Fogel, Ronen; Limson, Janice

doi:10.1016/j.electacta.2013.08.188

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…Chemometrics are employed to provide real-time analysis of variables that otherwise would require off-line analysis and interpretation using methods such as HPLC or GC/MS [19]. A few examples include in-situ biosensors [20][21][22], optical sensors [23,24], at-line implementations of traditional methods (HPLC, GC/MS) [19,25] and spectroscopic sensors [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42], among others.…”

Section: Towards Real-time Data Collectionmentioning

confidence: 99%

Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes

Gargalo

Udugama

Pontius

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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The biomanufacturing industry has now the opportunity to upgrade its production processes to be in harmony with the latest industrial revolution. Technology creates capabilities that enable smart manufacturing while still complying with unfolding regulations. However, many biomanufacturing companies, especially in the biopharma sector, still have a long way to go to fully benefit from smart manufacturing as they first need to transition their current operations to an information-driven future. One of the most significant obstacles towards the implementation of smart biomanufacturing is the collection of large sets of relevant data. Therefore, in this work, we both summarize the advances that have been made to date with regards to the monitoring and control of bioprocesses, and highlight some of the key technologies that have the potential to contribute to gathering big data. Empowering the current biomanufacturing industry to transition to Industry 4.0 operations allows for improved productivity through information-driven automation, not only by developing infrastructure, but also by introducing more advanced monitoring and control strategies.

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Section: Towards Real-time Data Collectionmentioning

confidence: 99%

Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes

Gargalo

Udugama

Pontius

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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“…9,11 Some of its contents may be electro-active, and other possible interferences can arise when catechol is added to the medium. Before applying the single two-step analytical procedure (as described earlier), an investigation of possible interferences from the cell tissue culture medium was carried out.…”

Section: Detection In Tissue Culture Mediamentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11] Thiols, such as homocysteine (HCy) and glutathione (GSH), are ubiquitous in physiological fluids as they play an important role in maintenance, antioxidation protection and metabolism. 9,[12][13] Levels of these thiols have been reported to be important as changes at the cellular role may have implications to conditions such as arteriosclerosis, 5,12,14 leukaemia, [14][15] cancer, 14,16 Alzheimer's, 5,[17][18][19] and/or Parkinson's disease.…”

Section: Introductionmentioning

confidence: 99%

Selective Thiol Detection in Authentic Biological Samples with the Use of Screen-printed Electrodes

Lee

Compton

2015

ANAL. SCI.

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A selective voltammetric determination of homocysteine and glutathione was applied to cell tissue culture media and human plasma via a single two-step method. The two-step method relies on the 1,4-Michael addition reaction between electro-oxidized catechol and the target thiol. Furthermore, the procedure relies on the differing reaction kinetics of the ortho-quinone with various thiol species giving different responses as a function of the scan rate. At faster scan rates homocysteine is only detected, while at slower scan rates the adduct signal reflects both homocysteine and glutathione. As a result, the quantification of both homocysteine and glutathione can be determined with a combination of both sets of data. The previous proof-of-concept (P. T. Lee, D. Lowinsohn, and R. G. Compton, Sensors, 2014, 14, 10395), is applied to the quantification of thiols in both tissue culture media and human plasma alone. Analytical parameters were determined for both homocysteine and glutathione in the respective media and the linear range. The sensitivities in tissue culture media are ca. 3 nA μM -1 and ca. 1 nA μM -1 and the limits of detections are ca. 2 μM and ca. 1 μM for homocysteine and glutathione, respectively. In human plasma, the sensitivities were determined to be 94 and 39 nA μM -1 , and the limit of detections are ca. 0.8 μM and ca. 0.8 μM for homocysteine and glutathione, respectively.

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“…Culture broth (red curve, formed by glucose 25 % and peptone 1 %) showed an oxidation peak around +0.6 V. The signal obtained was attributable to the peptone. Indeed, it is reported that glucose at this potential and with this kind of electrode is not able to give an electrochemical response [33]. On the other hand, the voltammogram corresponding to the culture medium with an addition of NADH (blue curve) showed the presence of two signals.…”

Section: Verification Of the Conceptmentioning

confidence: 88%

Electrochemical Glucose Quantification as a Strategy for Ethanolic Fermentation Monitoring

et al. 2019

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The possibility of using an electrochemical biosensor, using screen-printed electrodes modified with a carbonaceous material and a commercial kit for the determination of glucose, to monitor an ethanolic fermentation was analyzed. The determination is based on the electrochemical oxidation reaction of NADH that occurs at a potential where the components of the kit do not generate a current signal, even in the presence of the fermentation medium. The electrochemical system was used to analyze the variation of glucose concentration during a laboratory-scale fermentation. The results were contrasted with the variation of standard characterization parameters such as pH, total soluble solids (TSS), the viability of the yeast, and concentration of ethanol produced. Of these values, the total soluble solids should be related to the concentration of glucose obtained by the electrochemical sensor, however, this last measure is more specific for sugar since the TSS refers to all soluble solids. The obtained results allow us to verify the usefulness of the electrochemical method for real-time monitoring of a fermentation.

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Voltammetric investigation of complex growth media at a bare glassy carbon electrode: A case study of oxytetracycline

Cited by 7 publications

References 40 publications

Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes

Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes

Selective Thiol Detection in Authentic Biological Samples with the Use of Screen-printed Electrodes

Electrochemical Glucose Quantification as a Strategy for Ethanolic Fermentation Monitoring

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