Voltammetric measurements of aminosalicylate drugs using bismuth film electrode

Nigović, Biljana; Šimunić, Branimir; Hočevar, Samo B.

doi:10.1016/j.electacta.2009.05.006

Cited by 80 publications

(51 citation statements)

References 32 publications

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“…For chemical composition, 50 mg of LDH were dissolved with few drops of concentrated HNO 3 and diluted to 50 mL with deionized water. The amounts of Ni and Al in the obtained solution were then determined by FAAS.…”

Section: Resultsmentioning

confidence: 99%

“…A scanning electron microscope (SEM), model P Scan Vega 2 (Czech Republic), was additionally used to examine the morphological characteristics of the sorbent. Samples for elemental analysis were prepared by dissolving 50 mg of LDH with few drops of concentrated HNO 3 and diluting to 50 mL with deionized water, and Ni and Al analysis were performed by flame atomic absorption spectrometry (FAAS) with a Varian SpectrAA 220 (Mulgrave, Victoria, Australia) instrument. The pH values were measured with a Metrohm pH-meter (model 827, Switzerland), supplied with a glass-combined electrode.…”

Section: Apparatus and Instrumentsmentioning

confidence: 99%

“…All salts and compounds used for the interference study, NaOH, HCl (37%) and LDH precursors [i.e., purified nickel nitrate hexahydrate (Ni(NO 3 ) 2 • 6H 2 O, 99%) and aluminum nitrate nonahydrate (Al(NO 3 ) 3 • 9H 2 O, 99%)] were purchased from Merck (Darmstadt, Germany). The pH adjustments were performed with HNO 3 and NaOH (0.01-2.0 mol L −1 ) solutions. The pipettes and vessels used for the trace analysis were kept in 15% (v/v) nitric acid at least overnight and subsequently washed three times with deionized water prior to use.…”

Section: Standard Solutions and Reagentsmentioning

confidence: 99%

“…The pH of the solution was adjusted using minimum volume of 0.01 mol L -1 HNO 3 and/or NaOH. The total volume added for pH adjustment never exceeded 1% of the total volume.…”

Section: General Proceduresmentioning

confidence: 99%

“…Mesalamine is usually present at trace levels in a complex biological matrix, and the potentially interfering endogenous substances (which are usually present at higher concentrations than the drug) need to be removed before the analysis. Moreover, due to the presence of the primary aromatic amino group (-NH 3 + pKa = 6), carboxylic group (-COOH pKa = 3) and phenolic group (-OH pKa = 13.9) in the molecule, 18 mesalamine exhibits amphoteric properties, which (together with its high polarity) complicate its separation and detection. 10 In order to remove the interfering contribution from the biomatrices and to increase the selectivity and sensitivity of the analytical method, a sample preparation step is necessary prior to the instrumental analysis of mesalamine.…”

Section: Introductionmentioning

confidence: 99%

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Determination of mesalamine by spectrofluorometry in human serum after solid-phase extraction with Ni-Al layered double hydroxide as a nanosorbent

Abdolmohammad‐Zadeh¹,

Kohansal²

2012

J. Braz. Chem. Soc.

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Hidróxido duplo lamelar de níquel-alumínio nanoestruturado foi sintetizado e o potencial do material obtido, foi avaliado como sorvente para extração em fase sólida (SPE), na separação e pré-concentração de quantidades traço de mesalazina (ácido 5-aminossalicílico) usando o método de coluna. O analito retido em Ni-Al LDH foi removido por solução de NaOH e a concentração de 5-ASA eluída foi então determinada espectrometricamente em λ em = 480 nm com excitação em λ ex = 340 nm. Vários parâmetros experimentais afetando a eficiência da extração de mesalazina em Ni-Al (NO 3 -) LDH, tais como pH, quantidade de sorvente, taxa de fluxo da quantidade de amostra, condições de eluição e volume de amostra, foram investigados. Nas condições experimentais ideais, o limite de detecção e o fator de enriquecimento foram 0,04 e 40 µg L −1 , respectivamente. A curva de calibração do sistema de pré-concentração foi linear no intervalo de 0,1-45,0 µg L −1 com um coeficiente de correlação 0,998. O desvio padrão relativo resultante da análise de seis repetições de soluções de 100 mL contendo 1,0 µg L -1 de mesalazina foi 2,05%. O método otimizado foi aplicado com sucesso na determinação de mesalazina em amostras de soro de sangue. Nanostructured nickel-aluminum layered double hydroxide (Ni-Al LDH) was synthesized and the potential of the obtained material, as solid-phase extraction (SPE) sorbent, for separation and pre-concentration of trace amount of mesalamine (5-aminosalicylic acid) was assessed using column method. The retained analyte on Ni-Al LDH was eluted with NaOH solution and the concentration of the elueted 5-ASA was then spectrofluorometrically determined at λ em = 480 nm with excitation at λ ex = 340 nm. Various experimental parameters affecting the extraction efficiency of mesalamine on Ni-Al (NO 3 -) LDH, such as pH, amount of sorbent, sample loading flow rate, elution conditions and sample volume, were investigated. In the optimum experimental conditions, the limit of detection and enrichment factor were 0.04 and 40 µg L −1 , respectively. The calibration graph for the pre-concentration system was linear in the range of 0.1-45.0 µg L −1 with a correlation coefficient of 0.998. The relative standard deviation (RSD) resulting from the analysis of six replicates of 100 mL solutions containing 1.0 µg L -1 mesalamine was 2.05%. The optimized method was successfully applied to the determination of mesalamine in blood serum samples.

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Section: Resultsmentioning

confidence: 99%

Section: Apparatus and Instrumentsmentioning

confidence: 99%

Section: Standard Solutions and Reagentsmentioning

confidence: 99%

“…The pH of the solution was adjusted using minimum volume of 0.01 mol L -1 HNO 3 and/or NaOH. The total volume added for pH adjustment never exceeded 1% of the total volume.…”

Section: General Proceduresmentioning

confidence: 99%