Voltammetric Studies of 3‐Nitro‐tyrosine Electro‐oxidation at Solid Electrodes and its Interaction with DNA

Abstract

The electrochemical oxidation of 3‐nitro‐tyrosine (3‐NO2‐Tyr) was studied in aqueous media at metallic electrodes (platinum and gold), using voltammetric techniques. The interaction between 3‐NO2‐Tyr and double helix DNA (dsDNA) in a physiological medium was also investigated. Electro‐oxidation of 3‐NO2‐Tyr occurs in one single irreversible pH‐dependent step with the transfer of one electron and one proton from the phenolic group to the formation of radicals, which preferably dimerize, fouling the electrode surfaces. The differential pulse voltammetry and gel electrophoresis results clearly demonstrated a strong interaction of 3‐NO2‐Tyr with the dsDNA for the formation of a stable 3‐NO2‐Tyr‐dsDNA complex.

Modification of Zinc Tungstate with Functionalized Carbon Nanofibers for Electrochemical Detection of 3-Nitro-L-Tyrosine in Tap Water and Bovine Serum Albumin

“…4,5 3-Nitro-L-tyrosine (3-NLT) biomarker, a tyrosine derivative, is a chemical compound secreted by cancer tumorigenic cells. 6 3-NLT, an oxidative stress biomarker is produced due to the formation of a nitro group (-NO 2 ) at the third position of the tyrosine molecule and it is crucial to understand the levels of 3-NLT in biological systems also to develop the quantitative methods. 7,8 The early identification of this process may aid in treating major disorders that might become potentially dangerous in the future.…”