Volume regulation following hypotonic shock in isolated crypts of mouse distal colon

Mignen, Olivier; Gall, Christelle Le; Harvey, Brian; Thomas, Serge

doi:10.1111/j.1469-7793.1999.501ac.x

Cited by 40 publications

(34 citation statements)

References 26 publications

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“…As previously reported in other cell types (3,14,17,24) as well as in Mz-ChA-1 cells from human cholangiocarcinoma cell lines (23), normal mouse cholangiocytes exhibit an intact RVD, dependent on both Cl Ϫ and K ϩ conductances. As shown in Fig.…”

Section: Discussionsupporting

confidence: 52%

“…To assess changes in cell cluster volume during hypotonic challenge, we have used CSA as an indirect measure of volume, as done previously in other cell types by other investigators (17,19). The validity of CSA measurements of BDCCs by quantitative videomicroscopy as indirect measurements of cell cluster volume was confirmed by three independent methods: sequential light microscopy, fluorescence microscopy, and laser-scanning confocal microscopy of BCECFloaded BDCCs, each of which was followed by computer-assisted measurements of the corresponding CSAs and volumes.…”

Section: Discussionmentioning

confidence: 99%

“…As previously used for other cell types (17) and for hepatocytes and cholangiocytes (19), measurements of CSA have been used as indirect indexes of cell volumes in BDCCs. As shown in Fig.…”

Section: Characterization Of Bdccsmentioning

confidence: 99%

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Characterization of regulatory volume decrease in freshly isolated mouse cholangiocytes

Cho

2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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Cell volume regulation plays a vital role in many cell functions. Recent study indicates that both K+ and Cl− channels are important for the regulatory volume decrease (RVD) of cholangiocarcinoma cells, but its physiological significance is unclear due to the tumorous nature of the cells used. This present study reports the RVD of normal mouse cholangiocytes by using freshly isolated bile duct cell clusters (BDCC). A relatively simple and practical method of measuring the cross-sectional area of BDCCs by quantitative videomicroscopy was used to indirectly measure their volumes. Mouse cholangiocytes exhibited RVD, which was inhibited by 5-nitro-2′-(3-phenylpropylamino)-benzoate, DIDS, and glibenclamide, suggesting its dependence on certain chloride channels, such as volume-activated chloride channels. It is also inhibited by barium chloride but not by tetraethylammonium chloride, indicating its dependence on certain potassium channels. However, cAMP agonists had no significant effect on the RVD of BDCCs. This indirect method described can be used to study the RVD of cholangiocytes from normal as well as genetically altered mouse livers.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Characterization of regulatory volume decrease in freshly isolated mouse cholangiocytes

Cho

2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…9F) (1112). Leukotrienes have also been assigned a role in RVD in rat colon (193,646), and in release of inositol in glia cells (956). Finally, 12-LO metabolites were found to be involved in TRPV1 activation (395) and in RVD in human platelets (621).…”

Section: A Arachidonic Acid Metabolitesmentioning

confidence: 99%

Physiology of Cell Volume Regulation in Vertebrates

Hoffmann

Lambert²,

Pedersen³

2009

Physiological Reviews

1,306

1,677

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The ability to control cell volume is pivotal for cell function. Cell volume perturbation elicits a wide array of signaling events, leading to protective (e.g., cytoskeletal rearrangement) and adaptive (e.g., altered expression of osmolyte transporters and heat shock proteins) measures and, in most cases, activation of volume regulatory osmolyte transport. After acute swelling, cell volume is regulated by the process of regulatory volume decrease (RVD), which involves the activation of KCl cotransport and of channels mediating K+, Cl−, and taurine efflux. Conversely, after acute shrinkage, cell volume is regulated by the process of regulatory volume increase (RVI), which is mediated primarily by Na+/H+exchange, Na+-K+-2Cl−cotransport, and Na+channels. Here, we review in detail the current knowledge regarding the molecular identity of these transport pathways and their regulation by, e.g., membrane deformation, ionic strength, Ca2+, protein kinases and phosphatases, cytoskeletal elements, GTP binding proteins, lipid mediators, and reactive oxygen species, upon changes in cell volume. We also discuss the nature of the upstream elements in volume sensing in vertebrate organisms. Importantly, cell volume impacts on a wide array of physiological processes, including transepithelial transport; cell migration, proliferation, and death; and changes in cell volume function as specific signals regulating these processes. A discussion of this issue concludes the review.

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“…To assess cell volume changes with hypotonic challenges, we have used CSA as an indirect measure of cell volume as have other investigators for cholangiocytes (12) or for other cell types (13). In addition, we recently have shown and validated that the CSA measurements of mouse BDCCs obtained by quantitative videomicroscopy were highly correlated (linear correlation coefficient, r 2 Ͼ 0.94) with the cell volume measurements by 3 independent methods: 1) sequential Nomarski light, 2) fluorescence microscopy of BDCCs loaded with BCECF and computer-assisted measurements of the corresponding CSA and volumes, and 3) laser-scanning confocal microscopy of intracellular fluorescent dye, BCECF-loaded BDCCs, and computer-assisted three-dimensional reconstruction and volume calculation (4).…”

Section: Discussionmentioning

confidence: 99%

Impaired Regulatory Volume Decrease in Freshly Isolated Cholangiocytes from Cystic Fibrosis Mice

Cho

Siegrist

Zinzow

2004

Journal of Biological Chemistry

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Various K ؉ and Cl ؊ channels are important in cell volume regulation and biliary secretion, but the specific role of cystic fibrosis transmembrane conductance regulator in cholangiocyte cell volume regulation is not known. The goal of this research was to study regulatory volume decrease (RVD) in bile duct cell clusters (BDCCs) from normal and cystic fibrosis (CF) mouse livers. Mouse BDCCs without an enclosed lumen were prepared as described (Cho, W. K. (2002) Am. J. Physiol. 283, G1320 -G1327). The isotonic solution consisted of HEPES buffer with 40% of the NaCl replaced with isomolar amounts of sucrose, whereas hypotonic solution was the same as isotonic solution without sucrose. The cell volume changes were indirectly assessed by measuring cross-sectional area (CSA) changes of the BDCCs using quantitative videomicroscopy. Exposure to hypotonic solutions increased relative CSAs of normal BDCCs to 1.20 ؎ 0.01 (mean ؎ S.E., n ‫؍‬ 50) in 10 min, followed by RVD to 1.07 ؎ 0.01 by 40 min. Hypotonic challenge in CF mouse BDCCs also increased relative CSA to 1.20 ؎ 0.01 (n ‫؍‬ 53) in 10 min but without significant recovery. Coadministration of the K ؉ -selective ionophore valinomycin restored RVD in CF mouse BDCCs, suggesting that the impaired RVD was likely from a defect in K ؉ conductance. Moreover, this valinomycin-induced RVD in CF mice was inhibited by 5-nitro-2 -(3-phenylpropylamino)-benzoate, indicating that it is not from nonspecific effects. Neither cAMP nor calcium agonists could reverse the impaired RVD seen in CF cholangiocytes. Our conclusion is that CF mouse cholangiocytes have defective RVD from an impaired K ؉ efflux pathway, which could not be reversed by cAMP nor calcium agonists.Under physiological conditions, osmoregulation plays a crucial role in cholangiocytes, which are exposed to various osmotic stresses from the uptake of solutes and electrolytes and bile secretion (1, 2). A recent study on a human cholangiocarcinoma cell line (3), as well as our results in a study on primary bile duct cell clusters (BDCCs) 1 from normal mouse livers (4), indicates that cholangiocytes can regulate their cell volumes back to base line from hypotonicity-induced swelling. As in other cell types, these adaptive mechanisms of regulatory volume decrease (RVD) in cholangiocytes are mediated by certain K ϩ and Cl Ϫ conductances (3, 4).Cystic fibrosis (CF) is the most common inherited multisystem disease in the Caucasian population and is caused by a defect in the cystic fibrosis transmembrane conductance regulator (CFTR). Many CF patients develop a spectrum of hepatobiliary diseases that are thought to be due to secretory dysfunctions from the CFTR Cl Ϫ channel defects. In recent years, CF liver diseases have become the second most common cause of mortality in CF patients as they live longer, but the underlying pathophysiological mechanisms are not well understood. Recent immunocytochemical studies of the liver have shown that CFTR is expressed only on the bile duct epithelium but not on hepatocytes (5). The CFTR is...

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Volume regulation following hypotonic shock in isolated crypts of mouse distal colon

Cited by 40 publications

References 26 publications

Characterization of regulatory volume decrease in freshly isolated mouse cholangiocytes

Characterization of regulatory volume decrease in freshly isolated mouse cholangiocytes

Physiology of Cell Volume Regulation in Vertebrates

Impaired Regulatory Volume Decrease in Freshly Isolated Cholangiocytes from Cystic Fibrosis Mice

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