Volume Regulation in Epithelia

Larsen, Erik Hviid; Hoffmann, Else K.

doi:10.1007/978-1-4939-3366-2_4

Cited by 2 publications

(1 citation statement)

References 325 publications

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“…Larger animal models, such as rabbits, pigs, and dogs, have more similar eye size to human eyes, which allows the exploration of surgical techniques to administer DDSs, but anatomical and physiological differences remain remarkable [222,[226][227][228][229][230][231][232][233] and the costs of maintenance and facilities limit the use of large animals [222]. Rabbit have slender corneas, with fewer blinks and fewer tears [223,234,235]. They also lack PGA receptors and therefore the results of PGA-related studies based on rabbit models should be interpreted with caution [9].…”

Section: Challenges In Clinical Translationmentioning

confidence: 99%

Intraocular nano-microscale drug delivery systems for glaucoma treatment: design strategies and recent progress

Shen

Sun

2023

J Nanobiotechnol

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Glaucoma is a leading cause of irreversible visual impairment and blindness, affecting over 76.0 million people worldwide in 2020, with a predicted increase to 111.8 million by 2040. Hypotensive eye drops remain the gold standard for glaucoma treatment, while inadequate patient adherence to medication regimens and poor bioavailability of drugs to target tissues are major obstacles to effective treatment outcomes. Nano/micro-pharmaceuticals, with diverse spectra and abilities, may represent a hope of removing these obstacles. This review describes a set of intraocular nano/micro drug delivery systems involved in glaucoma treatment. Particularly, it investigates the structures, properties, and preclinical evidence supporting the use of these systems in glaucoma, followed by discussing the route of administration, the design of systems, and factors affecting in vivo performance. Finally, it concludes by highlighting the emerging notion as an attractive approach to address the unmet needs for managing glaucoma.

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Section: Challenges In Clinical Translationmentioning

confidence: 99%

Intraocular nano-microscale drug delivery systems for glaucoma treatment: design strategies and recent progress

Shen

Sun

2023

J Nanobiotechnol

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Role of cation-chloride cotransporters, Na/K-pump, and channels in cell water/ionic balance regulation under hyperosmolar conditions: in silico and experimental studies of opposite RVI and AVD responses of U937 cells to hyperosmolar media

Yurinskaya

2021

Preprint

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The work provides a modern mathematical description of animal cell electrochemical system under a balanced state and during the transition caused by an increase in external osmolarity, considering all the main ionic pathways in the cell membrane: the sodium pump, K+, Na+, Cl- electroconductive channels and cotransporters NC, KC, and NKCC. The description is applied to experimental data obtained on U937 cells cultured in suspension, which allows the required assays to be performed, including determination of cell water content using buoyant density, cell ion content using flame photometry, and optical methods using flow cytometry. The study of these cells can serve as a useful model for understanding the general mechanisms of regulation of cellular water and ionic balance, which cannot be properly analyzed in many important practical cases, such as ischemic disturbance of cellular ionic and water balance, when cells cannot be isolated. An essential part of the results is the developed software supplied with an executable file, which allows researchers with no programming experience to calculate unidirectional fluxes of monovalent ions through separate pathways and ion-electrochemical gradients that move ions through them, which is important for studying the functional expression of channels and transporters. It is shown how the developed approach is used to reveal changes in channels and transporters underlying the RVI and AVD responses to the hyperosmolar medium in the studied living U937 cells.

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Volume Regulation in Epithelia

Cited by 2 publications

References 325 publications

Intraocular nano-microscale drug delivery systems for glaucoma treatment: design strategies and recent progress

Intraocular nano-microscale drug delivery systems for glaucoma treatment: design strategies and recent progress

Role of cation-chloride cotransporters, Na/K-pump, and channels in cell water/ionic balance regulation under hyperosmolar conditions: in silico and experimental studies of opposite RVI and AVD responses of U937 cells to hyperosmolar media

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