Volume visualization for interactive microscopic image analysis

Voort, H. T. M. van der; Messerli, J. M.; Noordmans, Herke Jan; Smeulders, A.W.M.

doi:10.1002/1361-6374(199303)1:1<20::aid-bio5>3.3.co;2-u

Cited by 17 publications

(4 citation statements)

References 5 publications

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“…This results in removing fuzziness contained in the stack. In the present study, the maximum likelihood estimation algorithm was used throughout (24,25). Restored stacks were then further processed with Imarys (BitPlane, Zurich, Switzerland) for visualization and volume rendering.…”

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confidence: 99%

Characterization of TCL, a New GTPase of the Rho Family related to TC10 and Cdc42

Vignal¹,

Toledo²,

Comunale³

et al. 2000

Journal of Biological Chemistry

112

121

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GTPases of the Rho family control a wide variety of cellular processes such as cell morphology, motility, proliferation, differentiation, and apoptosis. We report here the characterization of a new Rho member, which shares 85% and 78% amino acid similarity to TC10 and Cdc42, respectively. This GTPase, termed as TC10-like (TCL) is encoded by an unexpectedly large locus, made of five exons spanning over 85 kilobases on human chromosome 14. TCL mRNA is 2.5 kilobases long and is mainly expressed in heart. In vitro, TCL shows rapid GDP/GTP exchange and displays higher GTP dissociation and hydolysis rates than TC10. Using the yeast twohybrid system and GST pull-down assays, we show that GTP-bound but not GDP-bound TCL protein directly interacts with Cdc42/Rac interacting binding domains, such as those found in PAK and WASP. Despite its overall similarity to TC10 and Cdc42, the constitutively active TCL mutant displays distinct morphogenic activity in REF-52 fibroblasts, producing large and dynamic Factin-rich ruffles on the dorsal cell membrane. Interestingly, TCL morphogenic activity is blocked by dominant negative Rac1 and Cdc42 mutants, suggesting a crosstalk between these three Rho GTPases.

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mentioning

confidence: 99%

Characterization of TCL, a New GTPase of the Rho Family related to TC10 and Cdc42

Vignal¹,

Toledo²,

Comunale³

et al. 2000

Journal of Biological Chemistry

112

121

View full text Add to dashboard Cite

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“…Our investigation aims to demonstrate the 3-D visualisation of the aforementioned helical structures. It has been shown that CLSM, besides providing clear identification of shapes of individual organized structures, allows the assignment of handness in more complex structures utilizing the x-y-z information coming with the optical sectioning techniques (Beltrame et al, 1992;Diaspro et al, 1996b;Messerli and Perrierd, 1995;Van der Voort et al, 1993;Willis et al, 1993) and a sort of stratigrafic technique such as the one utilized in scanning force microscopy by Samori and colleagues (1993).…”

Section: Discussionmentioning

confidence: 99%

Studies on the structure of sperm heads ofEledone cirrhosa by means of CLSM linked to bioimage-oriented devices

Diaspro¹,

Beltrame²,

Fato³

et al. 1997

Microsc. Res. Tech.

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We have used a confocal laser scanning optical microscope imaging device and a bioimage‐oriented workstation equipped for augmented reality to study the helical sperm head of the octopus Eledone cirrhosa. This approach allows us to study different complex organisational motifs due to the spatial arrangement of linear helical structures. We consider this helical specimen an enlarged copy of one of the most important biostructures governing cell functioning such as chromatin‐DNA. Moreover, this very same sample is made of highly compacted chromatin that can be studied at higher resolution, i.e., by means of scanning force microscopy. Fluorescence optical sectioning has been used to enter the spatial organisation. Three‐dimensional images of single, twisted, and folded fibers are shown. Microsc. Res. Tech. 36:159–164, 1997. © 1997 Wiley‐Liss, Inc.

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“…Furthermore, the isosurface was calculated for the volume data set using the same threshold. Calculation of the isosurface using the marching cubes algorithm (Lorensen and Cline 1987;Wilhelms and Van Gelder 1990;Thalmann and Thalmann 1991) results in a geometric object in contrast to commonly used volume rendering methods (Brakenhoff et al 1989; Van der Voort and Brakenhoff 1990;Messerli et al 1993; Van der Voort et al 1993;Lucas et al 1996). The calculation of the isosurface was repeated for the three individual data sets and a specific color was choosen for the three geometries, in particular, blue, red, and white for the cell extension [DiOC 6 (3)], the cathepsin B (Cy-3), and the collagen gel, respectively.…”

Section: Three-dimensional Reconstructionmentioning

confidence: 99%

Tomography of Cells by Confocal Laser Scanning Microscopy and Computer-assisted Three-dimensional Image Reconstruction: Localization of Cathepsin B in Tumor Cells Penetrating Collagen Gels In Vitro

Strohmaier

Porwol

Acker

et al. 1997

J Histochem Cytochem.

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SUMMARYWe used the nondestructive procedures of confocal laser scanning microscopy in combination with computer-assisted methods to visualize tumor cells in the process of penetrating collagen gels. Three independent sets of images were collected. The image information of all data sets was combined into one image, giving a three-dimensional (3D) impression at high light microscopic resolution and sensitivity. We collected information about the extracellular matrix using the reflection mode, the cell surface/morphology by staining with the fluorescent dye DiOC 6 (3), and the distribution of cathepsin B by Cy-3-labeled immunolocalization. The specific aim of our study was visualization of the spatial relationship of cell organelles as far as they contain the enzyme cathepsin B to cell morphology and motility in a 3D model of extracellular matrix. The majority of the enzyme was localized pericellularly, with no visible relationship to the direction of movement. However, substantial amounts also appeared in intramatrix pseudopodia and associated with the extracellular face of the plasma membane, which may be indicative either of secretion and/or epicellular activity. Our approach has general applicability to study of the spatial relationships of Biological structures have a dynamic three-dimensional (3D) organization. However, our understanding of structures and functions and their interrelations in cells is almost exclusively based on two-dimensional images and is therefore incomplete. In medical sciences, 3D imagery of whole organs or large anatomic regions of the body is possible by a number of noninvasive methods. Moreover, at the molecular level, spatial reconstitution of molecules or molecular assemblies is a common, although complicated procedure. At the cellular level, knowledge of spatial extension or interaction of structures at the light and electron microscopic level was obtained by destructive sectioning methods. Confocal laser scanning microscopy is now a well-established method to obtain optical sections of spatially extended biological objects (Brakenhoff et al. 1989;Masters and Farmer 1993;Wright et al. 1993). This precise and nondestructive procedure can render series of a sufficient number of equally thick sections to allow accurate 3D reconstitution of the imaged volume. The invention of appropriate fluorescent dyes and the development of specific antibodies to cell constituents are the last links in a chain leading to realistic 3D images of cells and their constituents.Such a methodology is an ideal tool to study cell locomotion (movement). In situ, this is a 3D process. It involves complex rearrangements of the intracellular compartments, intercellular interactions, and interactions of cells with the extracellular matrix. Movement of cells through natural barriers (invasion) is a basic phenomenon in living organisms. In higher organisms, such invasive processes occur during all stages of deCorrespondence to: Dr. Eberhard Spiess, Biomedizinische Strukturforschung 0195,

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Volume visualization for interactive microscopic image analysis

Cited by 17 publications

References 5 publications

Characterization of TCL, a New GTPase of the Rho Family related to TC10 and Cdc42

Characterization of TCL, a New GTPase of the Rho Family related to TC10 and Cdc42

Studies on the structure of sperm heads ofEledone cirrhosa by means of CLSM linked to bioimage-oriented devices

Tomography of Cells by Confocal Laser Scanning Microscopy and Computer-assisted Three-dimensional Image Reconstruction: Localization of Cathepsin B in Tumor Cells Penetrating Collagen Gels In Vitro

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