Volumetric Absorptive Microsampling (VAMS) for assaying immunosuppressants from venous whole blood by LC–MS/MS using a novel atmospheric pressure ionization probe (UniSpray™)

Paniagua-González, Lucía; Díaz‐Louzao, Carla; Lendoiro, Elena; Otero-Antón, Esteban; Cadarso-Suárez, C.; López-Rivadulla, Manuel; Cruz, Angelines; de-Castro-Ríos, Ana

doi:10.1016/j.jpba.2020.113422

Cited by 35 publications

(58 citation statements)

References 26 publications

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“…The disadvantage of TDM based on plasma samples is represented by the discomfort caused to patients to obtain venous samples by using sampling procedures that cannot be applied at all to pediatrics patients and/or newborns, limiting the widespread of TDM in such population. To overcome these issue, new microsampling devices are described in literature [34][35][36]. These systems allow to collect a known volume of blood by means of microfluidic system, nullifying the HCT and drop volume effects on the analytes determination [17,[19][20][21].…”

Section: Discussionmentioning

confidence: 99%

“…These systems allow to collect a known volume of blood by means of microfluidic system, nullifying the HCT and drop volume effects on the analytes determination [ 17 , 19 , 20 , 21 ]. Herein, we describe a new microsampling method for the simultaneous determination of THC and CBD using VAMS which has been demonstrated useful for the quantitative measurement of several drugs from venous and capillary blood including first-generation and third-generation antiseizure drugs [ 23 , 37 ], antibiotics [ 22 ] and immunosuppressants [ 35 , 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

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Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

et al. 2020

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The aim of this work is to evaluate volumetric absorptive microsampling (VAMS) from capillary blood as an alternative strategy for therapeutic drug monitoring (TDM) in patients treated with the newly available GW-purified form of cannabidiol (Epidiolex®). A fast ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) coupled to an online sample preparation system analysis was carried out on a Thermo Scientific Ultimate 3000 LC system coupled to a TSQ Quantiva triple quadrupole for the quantification of cannabidiol (CBD) and, in addition, delta-9-tetrahydrocannabinol (Δ9-THC). After validation using European Medicine Agency (EMA) guidelines the method was applied to samples obtained by finger prick of five pediatric patients treated with Epidiolex® and the results were compared to those obtained from venous blood and plasma. The method is linear in the range of 1–800 µg/L for both CBD and THC with intra- and inter-day precisions ranging from 5% to 14% and accuracies from −13% to +14% starting from 30 µL of sample. Stability in VAMS is ensured for up to 4 weeks at 25 °C thus allowing simple delivery. There was no difference (p = 0.69) between concentrations of CBD measured from VAMS sampled from capillary or venous blood (range: 52.19–330.14 or 72.15–383.45 µg/L) and those obtained from plasma (range: 64.3–374.09 µg/L) The VAMS-LC-MS/MS method represents a valid alternative strategy for therapeutic drug monitoring of patients treated with Epidiolex®.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

et al. 2020

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“…Several developments are being made to overcome the volume‐based bias leading to various novel techniques like Hemaxis, 37 hemaPEN, 38 Capitainer‐B, 39 Volumetric Absorptive Microsampling (VAMS)‐Mitra samplers, 40 Volumetric Absorptive Paper Disc (VAPD) and mini‐disc (VAPDmini), 41 Solid‐Phase Microextraction (SPME), 42 Fluispotter 43 and Tasso‐device 44 . The challenges are being eliminated, and precise analytical results are being obtained with innovative detection techniques 45–48 . Volumetrically derived dried blood microsampling continues to be a choice as it offers additional advantages of simplified logistics, preservation and transportation of samples.…”

Section: Microsamplingmentioning

confidence: 99%

“…44 The challenges are being eliminated, and precise analytical results are being obtained with innovative detection techniques. [45][46][47][48] Volumetrically derived dried blood microsampling continues to be a choice as it offers additional advantages of simplified 54 Another study demonstrated the application of DBS for the measurement of measles antibodies and vitamin A levels. The study was conducted by collecting DBS samples from 3316 individuals of age group between 0 and 49 years were collected for analysis of measles antibody.…”

Section: Microsamplingmentioning

confidence: 99%

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Microsampling: A role to play in Covid‐19 diagnosis, surveillance, treatment and clinical trials

Rajadhyaksha

Londhe

2021

Drug Testing and Analysis

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The outbreak of the new coronavirus disease changed the world upside down. Every day, millions of people were subjected to diagnostic testing for Covid‐19, all over the world. Molecular tests helped in the diagnosis of current infection by detecting the presence of viral genome whereas serological tests helped in detecting the presence of antibody in blood as well as contributed to vaccine development. This testing helped in understanding the immunogenicity, community prevalence, geographical spread and conditions post‐infection. However, with the contagious nature of the virus, biological specimen sampling involved the risk of transmission and spread of infection. Clinic or pathology visit was the most concerning part. Trained personnel and resources was another barrier. In this scenario, microsampling played an important role due to its most important advantage of remote, contactless, small volume and self‐sampling. Minimum requirements for sample storage and ease of shipment added value in this situation. The highly sensitive instruments and validated assay formats assured the accuracy of results and stability of samples. Microsampling techniques are contributing effectively to the Covid‐19 pandemic by reducing the demand for clinical staff in population‐level testing. The validated and established applications supported the use of microsampling in diagnosis, therapeutic drug monitoring, development of treatment or vaccines and clinical trials for Covid‐19.

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Development and validation of a rapid HPLC‐MS/MS method for simultaneous determination of cyclosporine A and tacrolimus in whole blood for routine therapeutic drug monitoring in organ transplantation

Han,

Liu,

et al. 2024

Rapid Comm Mass Spectrometry

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BackgroundTherapeutic drug monitoring is an integral part of organ transplantation. A rapid, simple, economical, and robust high‐performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) method for simultaneously determining the immunosuppressants cyclosporine A and tacrolimus might increase detection efficiency.MethodsIn this study, we developed and validated a rapid HPLC‐MS/MS method. Whole blood samples of 100 μL were prepared by protein precipitation with acetonitrile and 0.5 mol. L−1 ZnSO4. Chromatography was performed on a pre‐column using a gradient elution with 20 mmol. L−1 ammonium formate and 0.1% (v/v) formic acid in water (mobile phase A) and 0.1% (v/v) formic acid in methanol (mobile phase B) at a flow rate of 1.5 mL.min−1. The analysis time was 2.2 min. Electrospray ionization and multiple reaction monitoring were performed. The lower limit of quantification was set at 1 ng. L−1 for tacrolimus and 50 ng. L−1 for cyclosporine A.ResultsThe method showed adequate accuracy and precision with a sufficient linear range. The calibration curve range of tacrolimus and cyclosporine A was 1–30 and 50–1500 ng·mL‐1, respectively. All correlation coefficients were >0.99.ConclusionsThe developed HPLC‐MS/MS is rapid and can be used for simultaneous monitoring of tacrolimus and cyclosporine A.

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Volumetric Absorptive Microsampling (VAMS) for assaying immunosuppressants from venous whole blood by LC–MS/MS using a novel atmospheric pressure ionization probe (UniSpray™)

Cited by 35 publications

References 26 publications

Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

Microsampling: A role to play in Covid‐19 diagnosis, surveillance, treatment and clinical trials

Development and validation of a rapid HPLC‐MS/MS method for simultaneous determination of cyclosporine A and tacrolimus in whole blood for routine therapeutic drug monitoring in organ transplantation

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