Volumetric analysis of osteoclastic bioresorption of calcium phosphate ceramics with different solubilities

Winkler, Thomas; Hoenig, Elisa; Gildenhaar, Renate; Berger, Georg; Fritsch, Daniel S.; Janßen, Rolf; Morlock, Michael M.; Schilling, Arndt F.

doi:10.1016/j.actbio.2010.04.015

Cited by 38 publications

(29 citation statements)

References 27 publications

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“…Despite other reports of osteoclast resorption of CaP and specifically TCP, no characteristic osteoclast resorption pits could be found by SEM in this study (Yamada et al, 1997;Monchau et al, 2002;Wenisch et al, 2003;Xia et al, 2006;Winkler et al, 2010). Osteoclast resorption occurs exclusively in the sealing zone bounded by cytoskeletal actin rings (Teitelbaum, 2011).…”

Section: Nl Davison Et Al Surface Architecture Of Tricalcium Phosphatecontrasting

confidence: 53%

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Davison

Luo²,

Schoenmaker³

et al. 2014

eCM

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A current challenge of synthetic bone graft substitute design is to induce bone formation at a similar rate to its biological resorption, matching bone's intrinsic osteoinductivity and capacity for remodelling. We hypothesise that both osteoinduction and resorption can be achieved by altering surface microstructure of beta-tricalcium phosphate (TCP). To test this, two TCP ceramics are engineered with equivalent chemistry and macrostructure but with either submicron-or micron-scale surface architecture. In vitro, submicron-scale surface architecture differentiates larger, more active osteoclasts -a cell type shown to be important for both TCP resorption and osteogenesis -and enhances their secretion of osteogenic factors to induce osteoblast differentiation of human mesenchymal stem cells. In an intramuscular model, submicrostructured TCP forms 20 % bone in the free space, is resorbed by 24 %, and is densely populated by multinucleated osteoclast-like cells after 12 weeks; however, TCP with micron-scale surface architecture forms no bone, is essentially not resorbed, and contains scarce osteoclast-like cells. Thus, a novel submicron-structured TCP induces substantial bone formation and is resorbed at an equivalent rate, potentially through the control of osteoclast-like cells.

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Section: Nl Davison Et Al Surface Architecture Of Tricalcium Phosphatecontrasting

confidence: 53%

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Davison

Luo²,

Schoenmaker³

et al. 2014

eCM

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show abstract

“…As a consequence, TRAP-staining did not lead to any conclusive results. TRAP-positive staining similarly varies from reddish, purple, yellow and brown color (Hoshino et al, 2010;Jorgensen et al, 2002;Li et al, 2009b;Narducci et al, 2010;Newa et al, 2011;Paloneva et al, 2003;Schilling et al, 2004;Winkler et al, 2010). This low TRAP count was in clear contrast to light microscopy results (many multinucleated cells) and the bone resorption assay that featured distinct lacunar resorption throughout all groups.…”

Section: G Russmueller Et Al Effects Of Particulate Biomaterials On contrasting

confidence: 45%

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

Russmueller

Winkler²,

Lieber³

et al. 2017

eCM

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Much research has been done on bone cells, but only a few studies deal with biomaterial-induced effects on human osteoclasts, which may take on an important role in the successful regeneration of bone. In order to highlight such effects, human peripheral blood mononuclear cells (PBMCs) were extracted from venous blood, differentiated to osteoclasts and then cultured in, the presence of five particulate hydroxyapatite (HA)/β-tricalcium phosphate (TCP) biomaterials, on bovine bone slices and glass cover slips. The biomaterials, AlgOSS 50/50 (50 % HA/50 % TCP), AlgOSS 20/80 (20 % HA/80 % TCP), Algipore (98 % HA), Cerasorb (100 % TCP) and Bio-Oss (100 % HA) were chosen to assess their influence on cell morphology and numbers. Light microscopic evaluation was performed during ongoing cell culture. After 21 d of cultivation, the biomaterial-induced effects on osteoclastic resorption of the bone slices were evaluated by scanning electron microscopy (SEM). Osteoclast-like cells were identified by TRAP staining. All five biomaterials showed larger area fractions of resorbed bone than the control (5.6 ± 6.8 %), as measured on SEM images. The purely hydroxyapatite-based Algipore (9.8 ± 9.7 %) and Bio-Oss (7.9 ± 8.8 %) showed significantly elevated area fraction rates (p ≤ 0.05) of bone resorption. Light Microscope evaluation revealed a significant, but inhibiting effect of Cerasorb (p = 0.05). These data indicated that introducing of small biomaterial hydroxyapatite particles may have improved the performance of bone substitute materials.

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“…Cell-based resorption assay uses a variety of resorbing cells including primary and tumor osteoclasts, differentiated osteoclasts and monocyte/macrophage cell lines [15,16]. A limitation of cell-based assay is that the assay requires a flat surface for evaluating resorption lacuna or pits.…”

Section: Introductionmentioning

confidence: 99%

Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

et al. 2015

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Volumetric analysis of osteoclastic bioresorption of calcium phosphate ceramics with different solubilities

Cited by 38 publications

References 27 publications

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

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