Volumetric analysis of the periodontal microstructure under antiresorptive therapy: an experimental study in rabbits

Becker, Kathrin; Kerberger, Robert; Brunello, Giulia; Rockhoff, Joel; Rauch, Nicole; Schwarz, Frank

doi:10.1007/s00784-022-04503-z

Cited by 4 publications

(6 citation statements)

References 40 publications

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“…On the other hand, the ndings of the present and aforementioned analysis contradict the results of a former volumetric study, which assessed the periodontal microstructure of noninfected teeth in rabbits administered with Za 14 . In particular, signi cantly wider PLS were measured in animals administered with Za compared to the control group (i.e., non-Za) 14 . The differences between Za and non-Za groups were more apparent for the PLS measurements in the upper jaw.…”

Section: Discussioncontrasting

confidence: 99%

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

Ramanauskaite,

Mangold,

Padhye

et al. 2023

Preprint

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Objective The objective of this study was to investigate the influence of various antiresorptive and antiangiogenic medications on morphological changes in periodontal and oral tissue structures. Materials and methods Fifty-five Wistar rats randomly received dual application (i.e., at baseline and after 12-weeks) of the following medications: (1) amino-bisphosphonate [zoledronate (Zo)], (2) RANKL inhibitor [denosumab (De)], (3) antiangiogenic [bevacizumab (Be)], (4) Zo + Be, (5) De + Be or (6) no medication [Control (Co)]. Periodontal and oral tissue biopsies were obtained at 17 (n = 21 animals, Phase 1, (De = 3, De + Be = 3, Zo = 5, Be = 3, Zo + Be = 2, Co = 5) and 29 (n = 34 animals, (De = 8, De + Be = 6, Zo = 2, Be = 7, Zo + Be = 4, Co = 7, Phase 2) weeks after the second drug application. The following outcomes were histomorphometrically assessed: periodontal space width in the coronal (PLS-C, mm) and apical sections (PLS- A), number of empty alveolar bone lacunae in the coronal, apical sections and at the apex at respective tooth sites (EL – C, EL- A, EL- Ap), mucosal thickness at edentulous alveolar ridge areas (MT, mm), and, when present, associated areas of inflammatory cell infiltrates (ICI, mm2). Results Comparable mean PLS-C, PLS-A, ET-A, ET-C, ET-Ap, and MT values were observed in all experimental groups after Phases 1 and 2. The presence of ICI was identified in 3 animals in the Co group (Phase 1: 1, Phase 2: 2), and 17 animals in the test groups (Phase 1: 4; Phase 2: 14). The estimated ICI surface area was significantly higher in the Zo + Be group, followed by the Zo and Be groups compared to that measured in the Co group. The time (i.e., Phases 1 and 2) was not found to be a predictor for the extent of the ICI area. In all groups, the EL-C, EL-A, and EL-Ap values were significantly higher after Phase 2 compared to those assessed after Phase 1. The MT values were significantly reduced in all groups after Phase 2 compared to those measured after Phase 1. Conclusions The present analysis did not show any marked morphological effects of various antiresorptive and antiangiogenic medications on periodontal and oral tissue structures. The presence of inflammatory cell infiltrates was more frequently observed in the animals administered with antiresorptive and antiangiogenic medications as well as combinations thereof. Clinical relevance: Administration of antiresorptive and antiangiogenic medications may be capable of inducing inflammatory reactions in periodontal tissues.

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Section: Discussioncontrasting

confidence: 99%

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

Ramanauskaite,

Mangold,

Padhye

et al. 2023

Preprint

Self Cite

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show abstract

“…On the other hand, the findings of the present and aforementioned analysis contradict the results of a former volumetric study, which assessed the periodontal microstructure of noninfected teeth in rabbits administered with Za [ 14 ]. In particular, significantly wider PLS were measured in animals administered with Za compared to the control group (i.e., non-Za) [ 14 ]. The differences between Za and non-Za groups were more apparent for the PLS measurements in the upper jaw.…”

Section: Discussioncontrasting

confidence: 99%

“…In line with the radiographic findings noted in humans, one recent volumetric analysis of the periodontal and bone microstructure at noninfected teeth in rabbits repeatedly administrated with zoledronic acid (test group) also showed a marked increase in PLS, which was associated with a trend toward an increase in the adjacent trabecular bone thickness compared to the negative control group [ 14 ]. Similar outcomes were obtained in previous experimental studies employing rat models depicting an apparent correlation between the application of zoledronic acid and an increase in bone density, trabeculae thickness, and decreased alveolar bone vascularization at healthy teeth sites [ 15 , 16 ].…”

Section: Introductionsupporting

confidence: 54%

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

Ramanauskaite,

Mangold,

Padhye

et al. 2023

Clin Oral Invest

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Objective The objective of this study was to investigate the influence of various antiresorptive and antiangiogenic medications on morphological changes in periodontal and oral tissue structures. Materials and methods Fifty-five Wistar rats randomly received dual application (i.e., at baseline and after 12-weeks) one of the following medications: (1) amino-bisphosphonate [zoledronate (Zo)], (2) RANKL inhibitor [denosumab (De)], (3) antiangiogenic [bevacizumab (Be)], (4) Zo + Be, (5) De + Be or (6) no medication [Control (Co)]. Periodontal and oral tissue biopsies were obtained at 17 (n = 21 animals, Phase 1, (De = 3, De + Be = 3, Zo = 5, Be = 3, Zo + Be = 2, Co = 5) and 29 (n = 34 animals, (De = 8, De + Be = 6, Zo = 2, Be = 7, Zo + Be = 4, Co = 7, Phase 2) weeks after the second drug application. The following outcomes were histomorphometrically assessed: periodontal space width in the coronal (PLS-C, mm) and apical sections (PLS- A), number of empty alveolar bone lacunae in the coronal, apical sections and at the apex at respective tooth sites (EL – C, EL- A, EL- Ap), mucosal thickness at edentulous alveolar ridge areas (MT, mm), and, when present, associated areas of inflammatory cell infiltrates (ICI, mm2). Results Comparable mean PLS-C, PLS-A, ET-A, ET-C, ET-Ap, and MT values were observed in all experimental groups after Phases 1 and 2. The presence of ICI was identified in 3 animals in the Co group (Phase 1: 1, Phase 2: 2), and 17 animals in the test groups (Phase 1: 4; Phase 2: 14). The estimated ICI surface area was significantly higher in the Zo + Be group, followed by the Zo and Be groups compared to that measured in the Co group. The time (i.e., Phases 1 and 2) was not found to be a predictor for the extent of the ICI area. In all groups, the EL-C, EL-A, and EL-Ap values were significantly higher after Phase 2 compared to those assessed after Phase 1. The MT values were significantly reduced in all groups after Phase 2 compared to those measured after Phase 1. Conclusions The present evaluation was not able to find any morphological effects of different antiresorptive and antiangiogenic medications on periodontal and oral tissue structures. The presence of inflammatory cell infiltrates was more frequently observed in the animals administered with antiresorptive and antiangiogenic medications as well as combinations thereof. Clinical relevance Administration of antiresorptive and antiangiogenic medications may be capable of inducing inflammatory reactions in periodontal tissues.

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“…A recent preclinical study has focused on the analyses of periodontal and bone microstructures under Zo therapy [ 36 ]. In particular, at 10 months following a repeated drug administration (50μg/kg) in rabbits, Zo was associated with significantly higher periodontal space thickness (P.Th) values at non-infected maxillary premolars and 2nd molars when compared with the control (no Zo) group.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, at 10 months following a repeated drug administration (50μg/kg) in rabbits, Zo was associated with significantly higher periodontal space thickness (P.Th) values at non-infected maxillary premolars and 2nd molars when compared with the control (no Zo) group. While a trend for increased Tb.Th values in the Zo group was apparent, the analysis however did not reveal any significant between-group differences for mean BV/TV, BMD, Tb.Th, or BS/BV values [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of antiresorptive/antiangiogenic therapy on the extension of experimentally induced peri-implantitis lesions

et al. 2023

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Objectives To investigate the extension of experimentally induced peri-implantitis lesions under various antiresorptive and antiangiogenic medications. Material and methods Fourty-eight albino rats had randomly received the following medications (dual application, n = 8 each): (1) amino-bisphosphonate (zoledronate) (Zo), (2) RANKL inhibitor (denosumab) (De), (3) antiangiogenic (bevacizumab) (Be), (4) Zo+Be, (5) De+Be, or (6) no medication (Co). Ligature- and lipopolysaccharide-induced peri-implantitis lesions were established at 2 maxillary implants over a period of 16 weeks. Histological (e.g., apical extension and surface area of the inflammatory cell infiltrate—aICT, ICT; defect length; defect width; CD68 positive cells) and bone micromorphometric (μCT) outcomes were assessed. The animal was defined as a statistical unit. Results A total of n = 38 animals (Zo = 6, De = 6, Be = 8, Zo + Be = 6, De + Be = 5, Co = 7) were analyzed. ICT’s were commonly marked by a positive CD68 antigen reactivity. Comparable median aICT (lowest—Zo: 0.53 mm; highest—Be: 1.22 mm), ICT (lowest—De + Be: 0.00 mm2; highest—Co: 0.49 mm2), defect length (lowest—Zo: 0.90 mm; highest—Co: 1.93 mm) and defect width (lowest—De+Be: 1.27 mm; highest—Be: 1.80 mm) values were noted in all test and control groups. Within an inner (diameter: 0.8 mm) cylindric volume of interest, the bone microstructure did not significantly differ between groups. Conclusions The present analysis did not reveal any marked effects of various antiresorptive/ antiangiogenic medications on the extension of experimentally induced peri-implantitis lesions. Clinical relevance The extension of peri-implantitis lesions may not be facilitated by the antiresorptive and antiangiogenic medications investigated.

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Volumetric analysis of the periodontal microstructure under antiresorptive therapy: an experimental study in rabbits

Cited by 4 publications

References 40 publications

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

Influence of antiresorptive/antiangiogenic therapy on the extension of experimentally induced peri-implantitis lesions

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