Volumetric imaging of optically cleared and fluorescently labeled animal tissue (VIOLA) for quantifying the 3D biodistribution of nanoparticles at cellular resolution in tumor tissue

Rakhilin, Nikolai; Yang, Bing; Spilker, Mary E.; Manzuk, Lisa K.; Montgomery, Mary K.; Shin, Eyoung; Prashad, Nadira; Hwang, Jenn Jiang; Song, Young-Ho; Loganzo, Frank; Giddabasappa, Anand; Ram, Sripad

doi:10.1016/j.jconrel.2022.12.060

Journal of Controlled Release

2023

DOI: 10.1016/j.jconrel.2022.12.060

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Volumetric imaging of optically cleared and fluorescently labeled animal tissue (VIOLA) for quantifying the 3D biodistribution of nanoparticles at cellular resolution in tumor tissue

Nikolai Rakhilin

Bing Yang

Mary E. Spilker

et al.

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Cited by 2 publications

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“…O ptical fluorescence imaging is frequently applied to noninvasively track the biodistribution of fluorophore-labeled drugs, nanoparticles, and antibodies. [1][2][3][4][5] Commonly used fluorophores are near-infrared dyes such as heptamethine cyanine dyes. Planar fluorescence imaging methods are the most established techniques, but they do not consider absorption and scattering of organs and tissues and lack geometric information, making quantitative analysis difficult and prone to error.…”

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confidence: 99%

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CT- and MRI-Aided Fluorescence Tomography Reconstructions for Biodistribution Analysis

Schraven,

Brück,

Rosenhain

et al. 2023

Invest Radiol

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Objectives Optical fluorescence imaging can track the biodistribution of fluorophore-labeled drugs, nanoparticles, and antibodies longitudinally. In hybrid computed tomography–fluorescence tomography (CT-FLT), CT provides the anatomical information to generate scattering and absorption maps supporting a 3-dimensional reconstruction from the raw optical data. However, given the CT's limited soft tissue contrast, fluorescence reconstruction and quantification can be inaccurate and not sufficiently detailed. Magnetic resonance imaging (MRI) can overcome these limitations and extend the options for tissue characterization. Thus, we aimed to establish a hybrid CT-MRI-FLT approach for whole-body imaging and compared it with CT-FLT. Materials and Methods The MRI-based hybrid imaging approaches were established first by scanning a water and coconut oil–filled phantom, second by quantifying Cy7 concentrations of inserts in dead mice, and finally by analyzing the biodistribution of AF750-labeled immunoglobulins (IgG, IgA) in living SKH1 mice. Magnetic resonance imaging, acquired with a fat-water–separated mDixon sequence, CT, and FLT were co-registered using markers in the mouse holder frame filled with white petrolatum, which was solid, stable, and visible in both modalities. Results Computed tomography–MRI fusion was confirmed by comparing the segmentation agreement using Dice scores. Phantom segmentations showed good agreement, after correction for gradient linearity distortion and chemical shift. Organ segmentations in dead and living mice revealed adequate agreement for fusion. Marking the mouse holder frame and the successful CT-MRI fusion enabled MRI-FLT as well as CT-MRI-FLT reconstructions. Fluorescence tomography reconstructions supported by CT, MRI, or CT-MRI were comparable in dead mice with 60 pmol fluorescence inserts at different locations. Although standard CT-FLT reconstruction only considered general values for soft tissue, skin, lung, fat, and bone scattering, MRI's more versatile soft tissue contrast enabled the additional consideration of liver, kidneys, and brain. However, this did not change FLT reconstructions and quantifications significantly, whereas for extending scattering maps, it was important to accurately segment the organs and the entire mouse body. The various FLT reconstructions also provided comparable results for the in vivo biodistribution analyses with fluorescent immunoglobulins. However, MRI additionally enabled the visualization of gallbladder, thyroid, and brain. Furthermore, segmentations of liver, spleen, and kidney were more reliable due to better-defined contours than in CT. Therefore, the improved segmentations enabled better assignment of fluorescence signals and more differentiated conclusions with MRI-FLT. Conclusions Whole-body CT-MRI-FLT was implemented as a novel trimodal imaging approach, which allowed to more accurately assign fluorescence signals, thereby significantly improving pharmacokinetic analyses.

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confidence: 99%

“…Optical fluorescence imaging is frequently applied to noninvasively track the biodistribution of fluorophore-labeled drugs, nanoparticles, and antibodies 1–5 . Commonly used fluorophores are near-infrared dyes such as heptamethine cyanine dyes.…”

mentioning

confidence: 99%

CT- and MRI-Aided Fluorescence Tomography Reconstructions for Biodistribution Analysis

Schraven,

Brück,

Rosenhain

et al. 2023

Invest Radiol

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Strategies and methods of nanocrystal technology for targeting drug delivery

Xue,

Ding,

Liu

et al. 2024

J Nanopart Res

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Volumetric imaging of optically cleared and fluorescently labeled animal tissue (VIOLA) for quantifying the 3D biodistribution of nanoparticles at cellular resolution in tumor tissue

Cited by 2 publications

References 60 publications

CT- and MRI-Aided Fluorescence Tomography Reconstructions for Biodistribution Analysis

CT- and MRI-Aided Fluorescence Tomography Reconstructions for Biodistribution Analysis

Strategies and methods of nanocrystal technology for targeting drug delivery

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