Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter

Drobek, Christoph; Meyer, Juliane; Mau, R. Vinh; Wolff, Anne; Peters, Kirsten; Seitz, Hermann

doi:10.1016/j.isci.2022.105796

Cited by 3 publications

References 34 publications

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Modeling and simulation of dielectrophoretic sorting of tenogenically differentiating mesenchymal stem cells for high throughput

Oladokun,

Srivastava,

Schiele

et al. 2024

Physics of Fluids

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Mesenchymal stem cell (MSC)-based regenerative therapies are promising for healing tendon injuries and tears, due to their potential to differentiate into tenogenic cells. However, generating homogeneous populations of tenogenically differentiated stem cells remains a big challenge, as non-differentiated cells can lead to post-transplantation complications. Therefore, a homogenous sample of tenogenically differentiated MSCs is critical for advancing tendon therapies and avoiding uncontrolled cell growth or non-tendon tissue formation (e.g., ectopic bone). This work is focused on designing and simulating a dielectrophoretic (DEP)-based label-free, microfluidic platform to selectively sort and enrich tenogenically differentiated MSCs (tMSCs) from undifferentiated MSCs. Using particle tracing, creeping flow (transport of diluted species model), and electric current physics modules in the COMSOL Multiphysics simulation software package, the sorting was simulated within a two-stage microfluidic device operating at a sinusoidal frequency of 160 kHz. The optimal separation efficiency and purity are achieved at an inlet velocity of 400–1000 μm/s, with specific voltage configurations, enabling recovery of one million tMSCs in ∼3 h. Results demonstrate a near-linear relation between recovery time and particle count at the outlet boundaries and selected surfaces, indicating consistent throughput across varying conditions. This study demonstrates that DEP can offer a scalable, efficient, and label-free method for enriching tMSC populations with high selectivity, enhancing more prospects for MSC-based tendon therapies and advancing the development of microfluidic sorting devices for regenerative medicine applications.

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Modeling and simulation of dielectrophoretic sorting of tenogenically differentiating mesenchymal stem cells for high throughput

Oladokun,

Srivastava,

Schiele

et al. 2024

Physics of Fluids

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A unique spontaneously immortalised cell line from pig with enhanced adipogenic capacity - a foundational tool for cellular agriculture

Thrower,

Riley,

Lee

et al. 2024

Preprint

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Cultivated meat promises to address some of the pressing challenges associated with large-scale production of animals for food. An important limitation to realising such promise is the lack of readily available cell lines that can be expanded robustly for scale-up culture while maintaining the capacity to differentiate into tissues of interest, namely fat and muscle. Here, we report a porcine mesenchymal stem cell line (FaTTy) which, uniquely, upon spontaneously immortalisation acquired enhanced adipogenic efficiency, close to 100%, that has now been maintained for over 200 population doublings. FaTTy is able to differentiate with high efficiency in both 2D and 3D contexts as well as in the absence of serum, and produces mature adipocytes upon prolonged differentiation. Moreover, FaTTy adipocytes display fatty acid profiles largely similar to native pig fat but with higher monounsaturated-to-saturated ratios. FaTTy displays minor aneuploidy, characterised by lack of Y chromosome, and lacks typical genetic or functional properties of tumorigenic cells. These highly distinctive characteristics, together with its non-genetically modified nature, make FaTTy an extremely attractive, potentially game-changing resource for food manufacturing, and particularly cultivated meat.

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Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration

Mand,

Hahn,

Meyer

et al. 2024

Bioengineering

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Within the healthy human body, cells reside within the physiological environment of a tissue compound. Here, they are subject to constant low levels of mechanical stress that can influence the growth and differentiation of the cells. The liposuction of adipose tissue and the subsequent isolation of mesenchymal stem/stromal cells (MSCs), for example, are procedures that induce a high level of mechanical shear stress. As MSCs play a central role in tissue regeneration by migrating into regenerating areas and driving regeneration through proliferation and tissue-specific differentiation, they are increasingly used in therapeutic applications. Consequently, there is a strong interest in investigating the effects of shear stress on MSCs. In this study, we present a set-up for applying high shear rates to cells based on a rotational rheometer with a small-angle cone–plate configuration. This set-up was used to investigate the effect of various shear stresses on human adipose-derived MSCs in suspension. The results of the study show that the viability of the cells remained unaffected up to 18.38 Pa for an exposure time of 5 min. However, it was observed that intense shear stress damaged the cells, with longer treatment durations increasing the percentage of cell debris.

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Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter

Cited by 3 publications

References 34 publications

Modeling and simulation of dielectrophoretic sorting of tenogenically differentiating mesenchymal stem cells for high throughput

Modeling and simulation of dielectrophoretic sorting of tenogenically differentiating mesenchymal stem cells for high throughput

A unique spontaneously immortalised cell line from pig with enhanced adipogenic capacity - a foundational tool for cellular agriculture

Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration

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